Title:
PROCESS FOR PRODUCING HEAVY ATOM DERIVATIVE USING VAPORIZED IODINE, METHOD FOR COLLECTING PHASE INFORMATION, AND METHOD FOR DETERMINING THREE-DIMENSIONAL STRUCTURE OF PROTEIN CRYSTAL
Document Type and Number:
WIPO Patent Application WO/2007/023811
Kind Code:
A1
Abstract:
[PROBLEMS] To provide a method for preparing a heavy atom derivative of a protein crystal in a simple manner with high probability, a method for collecting phase information, and a method for determining a three-dimensional structure of a protein crystal. [MEANS FOR SOLVING PROBLEMS] The method for preparing a heavy atom derivative of a protein crystal comprises allowing iodine to act on a protein crystal. Vaporized iodine and hydrogen peroxide are allowed to act on the protein crystal to prepare a heavy atom derivative. The method for collecting phase information comprises subjecting the heavy atom derivative of the protein crystal prepared using iodine by the above method to a X-ray diffraction test to collect phase information of diffraction X-ray from the heavy atom derivative. The method for determining a three-dimensional structure of a protein crystal comprises determining the three-dimensional structure of a protein crystal based on the phase information of diffraction X-ray from the heavy atom derivative collected by this method.
Inventors:
MIYATAKE HIDEYUKI (JP)
Application Number:
PCT/JP2006/316414
Publication Date:
March 01, 2007
Filing Date:
August 22, 2006
Export Citation:
Assignee:
RIKEN (JP)
MIYATAKE HIDEYUKI (JP)
MIYATAKE HIDEYUKI (JP)
International Classes:
C07K1/13; C07K14/43; G01N23/20; C07B59/00
Foreign References:
| DE2227439A1 | 1974-01-03 | |||
| JPS6048937A | 1985-03-16 | |||
| JP2005298426A | 2005-10-27 |
Other References:
EVANS G. ET AL: "Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment", ACTA CRYSTALLOGRAPHICA, SECTION D: BIOLOGICAL CRYSTALLOGRAPHY, vol. D58, no. 6,2, 2002, pages 976 - 991, XP008080690
Attorney, Agent or Firm:
SIKs & Co. (Kyobashi-Nisshoku Bldg., 8-7, Kyobashi 1-chome, Chuo-k, Tokyo 31, JP)
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