PROCESS FOR PRODUCING L-METHIONINE FROM O-PHOSPHO-L-HOMOSERINE AND METHANETHIOL EMPLOYING A MUTATED CYSTATHIONINE GAMMA-SYNTHASE

Title:

PROCESS FOR PRODUCING L-METHIONINE FROM O-PHOSPHO-L-HOMOSERINE AND METHANETHIOL EMPLOYING A MUTATED CYSTATHIONINE GAMMA-SYNTHASE

Document Type and Number:

WIPO Patent Application WO/2014/064244

Kind Code:

A3

Abstract:

Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3P04. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyse the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4.

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Inventors:

MERKAMM MURIEL (FR)
LOUIS DOMINIQUE (FR)
JAILLARDON KARINE (FR)
THOMAS DOMINIQUE (FR)
MARLIERE PHILIPPE (BE)

Application Number:

PCT/EP2013/072380

Publication Date:

August 14, 2014

Filing Date:

October 25, 2013

Export Citation:

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Assignee:

ADISSEO FRANCE SAS (FR)

International Classes:

C12P13/12; C12N9/10; C12N15/10; C12P11/00; C12P19/40; C12P21/02

Domestic Patent References:

WO1993017112A1	1993-09-02
WO2011031285A1	2011-03-17

Other References:

KREFT, B.D. ET AL.: "Purification and Properties of Cystathionine gamma-Synthase from Wheat (Triticum aestivum L.)", PLANT PHYSIOLOGY, vol. 104, no. 4, 1994, pages 1215 - 1220, XP002173650
VARTANIAN, J.-P. ET AL.: "Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions", NUCLEIC ACIDS RESEARCH, vol. 24, no. 14, 1996, pages 2627 - 2631, XP002713199

Attorney, Agent or Firm:

VOSSIUS & PARTNER (München, DE)

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