Title:
PROCESS FOR PRODUCING MODIFIED PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2003/084983
Kind Code:
A1
Abstract:
A process for producing a peptide or a protein containing an amino acid residue having a modified side chain characterized by comprising chemically producing a peptide fragment containing an amino acid residue having a modified side chain with the use of a weakly acidic eliminating resin, producing by a genetic modification method and/or an enzymatic method another peptide fragment containing no amino acid residue having a modified side chain, and then fusing the obtained two peptides fragments with each other. According to this process, a peptide or a protein having a modification such as acylation, glycosylation or phosphorylation and being excellent in qualities can be efficiently obtained.
Inventors:
MINAMITAKE YOSHIHARU (JP)
MATSUMOTO MASARU (JP)
MAKINO TOMOHIRO (JP)
MATSUMOTO MASARU (JP)
MAKINO TOMOHIRO (JP)
Application Number:
PCT/JP2003/004590
Publication Date:
October 16, 2003
Filing Date:
April 10, 2003
Export Citation:
Assignee:
DAIICHI SUNTORY PHARMA CO LTD (JP)
KANGAWA KENJI (JP)
MINAMITAKE YOSHIHARU (JP)
MATSUMOTO MASARU (JP)
MAKINO TOMOHIRO (JP)
KANGAWA KENJI (JP)
MINAMITAKE YOSHIHARU (JP)
MATSUMOTO MASARU (JP)
MAKINO TOMOHIRO (JP)
International Classes:
C07K1/04; C07K1/107; C07K9/00; C07K14/46; C07K14/465; C07K14/60; (IPC1-7): C07K1/04; C07K1/06; C07K14/46; C07K14/465; C07K9/00
Domestic Patent References:
| WO2000071570A1 | 2000-11-30 | |||
| WO2001007475A1 | 2001-02-01 |
Other References:
KITAGAWA K. ET AL.: "Facile solid-phase synthesis of sulfated tyrosine-containing peptides: Part II. Total synthesis of human big gastrin-II and its C-terminal glycine-extended peptide(G34-Gly sulfate) by the solid-phase segment condensation approach", CHEM. PHARM. BULL., vol. 49, no. 8, 2001, pages 958 - 963, XP002971319
BARLOS K. ET AL.: "Application of 2-chlorotrityl resin in solid phase synthesis of Leu15)-gastrin I and unsulfated cholecystokinin octapeptide. Selective O-deprotection of tyrosine", INT. J. PEPT. PROTEIN RES., vol. 38, no. 6, 1991, pages 555 - 561, XP000244135
BREDNAREK M.A. ET AL.: "Structure-function studies on the new growth hormone-releasing peptide, ghrelin: minimal sequence of ghrelin necessary for activation of growth hormone secretagogue receptor 1a", J. MED. CHEM., vol. 43, no. 23, 2000, pages 4370 - 4376, XP002166937
KOJIMA M. ET AL.: "Ghrelin is a growth-hormone-releasing acylated peptide from stomach", NATURE, vol. 402, no. 6762, 1999, pages 656 - 660, XP002196445
ISHIMARU M. ET AL.: "Stability of the octanoyl group essential for expressing the biological activities of rat ghrelin", PEPTIDE SCIENCE, vol. 2002, no. 39, March 2003 (2003-03-01), pages 157 - 160, XP002971320
See also references of EP 1493747A4
BARLOS K. ET AL.: "Application of 2-chlorotrityl resin in solid phase synthesis of Leu15)-gastrin I and unsulfated cholecystokinin octapeptide. Selective O-deprotection of tyrosine", INT. J. PEPT. PROTEIN RES., vol. 38, no. 6, 1991, pages 555 - 561, XP000244135
BREDNAREK M.A. ET AL.: "Structure-function studies on the new growth hormone-releasing peptide, ghrelin: minimal sequence of ghrelin necessary for activation of growth hormone secretagogue receptor 1a", J. MED. CHEM., vol. 43, no. 23, 2000, pages 4370 - 4376, XP002166937
KOJIMA M. ET AL.: "Ghrelin is a growth-hormone-releasing acylated peptide from stomach", NATURE, vol. 402, no. 6762, 1999, pages 656 - 660, XP002196445
ISHIMARU M. ET AL.: "Stability of the octanoyl group essential for expressing the biological activities of rat ghrelin", PEPTIDE SCIENCE, vol. 2002, no. 39, March 2003 (2003-03-01), pages 157 - 160, XP002971320
See also references of EP 1493747A4
Attorney, Agent or Firm:
Iwatani, Ryo (1-27 Dojima 2-chome, Kita-k, Osaka-shi Osaka, JP)
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