Title:
PROCESS FOR PRODUCING VITAMIN B¿12? FROM HYDROGEN-METABOLIZING METHANE BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2001/057231
Kind Code:
A1
Abstract:
Vitamin B¿12? is efficiently produced at a large content and a high yield by acclimatizing a mesophilic methane bacterium obtained from digested sludge in an H¿2?/CO¿2? medium; growing the thus acclimatized methane bacterium on a support containing inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor; allowing the bacterium to metabolize a mixed gas, which is prepared by adding hydrogen to at least one of coal gas and biogas, into methane; and, at the same time, recovering cobalamin contained in the fermented liquor as an extracellular product in the form of cyanocobalamin by using potassium cyanide.
Inventors:
MAEKAWA TAKAAKI (JP)
ZHANG ZHEN YA (JP)
ZHANG ZHEN YA (JP)
Application Number:
PCT/JP2001/000720
Publication Date:
August 09, 2001
Filing Date:
February 01, 2001
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
MAEKAWA TAKAAKI (JP)
ZHANG ZHEN YA (JP)
MAEKAWA TAKAAKI (JP)
ZHANG ZHEN YA (JP)
International Classes:
C12P3/00; C12P5/02; C12P19/42; C12M1/40; (IPC1-7): C12P19/42; C12P3/00; C12P5/02; C12M1/40
Foreign References:
JPH06169783A | 1994-06-21 | |||
JPH044892A | 1992-01-09 | |||
EP0881287A2 | 1998-12-02 | |||
JPH02286093A | 1990-11-26 | |||
JPH0423979A | 1992-01-28 | |||
JPH09140181A | 1997-05-27 | |||
JPH10327850A | 1998-12-15 |
Other References:
SHIRO NAGAHISA ET AL., SEIBUTSU- KOGAKU KAISHI, vol. 76, no. 6, 1998, pages 447 - 455
See also references of EP 1264895A4
See also references of EP 1264895A4
Attorney, Agent or Firm:
Nishizawa, Toshio (37-10 Udagawa-ch, Shibuya-cho Tokyo, JP)
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