Title:
PROCESS FOR PRODUCTION OF INDUCED PLURIPOTENT STEM CELL
Document Type and Number:
WIPO Patent Application WO/2011/158852
Kind Code:
A1
Abstract:
Disclosed is a method for producing an induced pluripotent stem cell in a simple manner and with high efficiency without the need of inserting a cell reprogramming factor into the genome of the induced pluripotent stem cell. It is found that an induced pluripotent stem cell can be produced in a safe and simple manner and with high efficiency by preparing a cell having, introduced therein, DNA encoding a fusion protein comprising an extracellular secretion signal, a protease-recognizing sequence, a protein introduction domain and a cell reprogramming factor and introducing the fusion protein secreted from the prepared cell into a somatic cell.
Inventors:
NAKAUCHI HIROMITSU (JP)
OTSU MAKOTO (JP)
TAKAYAMA NAOYA (JP)
AHN DONG-HYUCK (JP)
OTSU MAKOTO (JP)
TAKAYAMA NAOYA (JP)
AHN DONG-HYUCK (JP)
Application Number:
PCT/JP2011/063650
Publication Date:
December 22, 2011
Filing Date:
June 15, 2011
Export Citation:
Assignee:
UNIV TOKYO (JP)
NAKAUCHI HIROMITSU (JP)
OTSU MAKOTO (JP)
TAKAYAMA NAOYA (JP)
AHN DONG-HYUCK (JP)
NAKAUCHI HIROMITSU (JP)
OTSU MAKOTO (JP)
TAKAYAMA NAOYA (JP)
AHN DONG-HYUCK (JP)
International Classes:
C12N5/071; C12N5/10; C12N15/09
Foreign References:
| JP2010110289A | 2010-05-20 |
Other References:
KIM, D. ET AL.: "Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.", CELL STEM CELL, vol. 4, no. 6, 5 June 2009 (2009-06-05), pages 472 - 476
ZHOU, H. ET AL.: "Generation of induced pluripotent stem cells using recombinant proteins.", CELL STEM CELL, vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 384
PAN, C. ET AL.: "Reprogramming human fibroblasts using HIV-1 TAT recombinant proteins OCT4, SOX2, KLF4 and c-MYC.", MOL. BIOL. REP., vol. 37, no. 4, April 2010 (2010-04-01), pages 2117 - 2124
MCMAHON, A.P. ET AL.: "The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain.", CELL, vol. 62, no. 6, 21 September 1990 (1990-09-21), pages 1073 - 1085
ZHOU, H. ET AL.: "Generation of induced pluripotent stem cells using recombinant proteins.", CELL STEM CELL, vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 384
PAN, C. ET AL.: "Reprogramming human fibroblasts using HIV-1 TAT recombinant proteins OCT4, SOX2, KLF4 and c-MYC.", MOL. BIOL. REP., vol. 37, no. 4, April 2010 (2010-04-01), pages 2117 - 2124
MCMAHON, A.P. ET AL.: "The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain.", CELL, vol. 62, no. 6, 21 September 1990 (1990-09-21), pages 1073 - 1085
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
Patent business corporation cent Crest international patent firm (JP)
Patent business corporation cent Crest international patent firm (JP)
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