Title:
PROCESS FOR PRODUCTION OF POLYPEPTIDE BY REGULATION OF ASSEMBLY
Document Type and Number:
WIPO Patent Application WO/2006/106905
Kind Code:
A1
Abstract:
It is found that the assembly of polypeptides can be inhibited by altering an amino acid residue that forms the interface between the polypeptides in the assembly into another amino acid residue having the same charge as that of the amino acid residue. Thus, it becomes possible to form a hetero molecule with good efficiency. The present process can be suitably used, for example, in the production of a bispecific antibody.
Inventors:
IGAWA TOMOYUKI (JP)
TSUNODA HIROYUKI (JP)
TSUNODA HIROYUKI (JP)
Application Number:
PCT/JP2006/306803
Publication Date:
October 12, 2006
Filing Date:
March 31, 2006
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
IGAWA TOMOYUKI (JP)
TSUNODA HIROYUKI (JP)
IGAWA TOMOYUKI (JP)
TSUNODA HIROYUKI (JP)
International Classes:
C12N15/09; A61K39/395; C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Domestic Patent References:
| WO1997010354A1 | 1997-03-20 |
Foreign References:
| JP2004086862A | 2004-03-18 | |||
| JPH11500916A | 1999-01-26 | |||
| JPH08500979A | 1996-02-06 |
Other References:
SAL-MAN N. ET AL.: "Arginine mutations within a transmembrane domain of Tar, an Escherichia coli aspartate receptor, can drive homodimer dissociation and heterodimer association in vivo", BIOCHEM. J., vol. 385, no. PART 1, January 2005 (2005-01-01), pages 29 - 36, XP003005868
KUMAR R. ET AL.: "The second PDZ domain of INAD is a type I domain involved in binding to eye protein kinase C. Mutational analysis and naturally occurring variants", J. BIOL. CHEM., vol. 276, no. 27, 2001, pages 24971 - 24977, XP003005869
MAITY H. ET AL.: "Equilibrium unfolding of dimeric and engineered monomeric forms of lambda Cro (F58W) repressor and the effect of added salts: evidence for the formation of folded monomer induced by sodium perchlorate", ARCH. BIOCHEM. BIOPHYS., vol. 434, no. 1, 1 February 2005 (2005-02-01), pages 93 - 107, XP004699355
LIU X.Y. ET AL.: "Functional interactions between arginine-133 and aspartate-88 in the human reduced folate carrier: evidence for a charge-pair association", BIOCHEM. J., vol. 358, no. PART, 2001, pages 511 - 516, XP003005870
KABAT EA ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NIH
ALT M ET AL., FEBS LETTERS, vol. 454, 1999, pages 90 - 94
BLOOD, vol. 105, 2005, pages 562 - 566
See also references of EP 1870459A4
KUMAR R. ET AL.: "The second PDZ domain of INAD is a type I domain involved in binding to eye protein kinase C. Mutational analysis and naturally occurring variants", J. BIOL. CHEM., vol. 276, no. 27, 2001, pages 24971 - 24977, XP003005869
MAITY H. ET AL.: "Equilibrium unfolding of dimeric and engineered monomeric forms of lambda Cro (F58W) repressor and the effect of added salts: evidence for the formation of folded monomer induced by sodium perchlorate", ARCH. BIOCHEM. BIOPHYS., vol. 434, no. 1, 1 February 2005 (2005-02-01), pages 93 - 107, XP004699355
LIU X.Y. ET AL.: "Functional interactions between arginine-133 and aspartate-88 in the human reduced folate carrier: evidence for a charge-pair association", BIOCHEM. J., vol. 358, no. PART, 2001, pages 511 - 516, XP003005870
KABAT EA ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NIH
ALT M ET AL., FEBS LETTERS, vol. 454, 1999, pages 90 - 94
BLOOD, vol. 105, 2005, pages 562 - 566
See also references of EP 1870459A4
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-machi Tsuchiura-sh, Ibaraki 47, JP)
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