PROCESS FOR SEPARATING OR EXTRACTING NUCLEIC ACID FROM SPECIMEN CONTAINING THE SAME

Title:

PROCESS FOR SEPARATING OR EXTRACTING NUCLEIC ACID FROM SPECIMEN CONTAINING THE SAME

Document Type and Number:

WIPO Patent Application WO/1992/018647

Kind Code:

A1

Abstract:

A process for separating or extructing a specified nucleic acid from the solution of a specimen containing nucleic acids which comprises the steps of: (1) immobilizing a single-stranded nucleic acid on an insoluble carrier at its terminal, (2) contacting the immobilized nucleic acid with the specimen containing nucleic acids in solution under the nucleic acid hybridization condition, and (3) isolating the nucleic acid which hybridizes with the immobilized nucleic acid from the specimen. This process can realize simultaneous separation of two or more single-stranded nucleic acids which are different from one another in the base sequence and highly accurate nucleic acid subtraction.

Inventors:

KISHIMOTO TOSHIHIKO (JP)
NIWA SHIN-ICHIRO (JP)
TSURUI HIROMICHI (JP)

Application Number:

PCT/JP1992/000473

Publication Date:

October 29, 1992

Filing Date:

April 15, 1992

Export Citation:

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Assignee:

SUMITOMO ELECTRIC INDUSTRIES (JP)

International Classes:

C12N15/10; C12Q1/68; (IPC1-7): C12N15/10; C12Q1/68

Foreign References:

JPS6131100A	1986-02-13
JPS62265999A	1987-11-18
JPS61227785A	1986-10-09

Other References: