PRODRUGS OF DIPHENYL OX- INDOL- 2 -ONE COMPOUNDS FOR THE TREATMENT OF CANCERS

FIELD OF THE INVENTION

The present invention relates to novel prodrugs of substituted 3,3-diphenyl-l,3- dihydro-indol-2-one compounds.

BACKGROUND OF THE INVENTION

US 1,624,675 describes O-0-diacyl derivatives of diphenolisatine and that these compounds possess laxative properties.

US 2004/0242563 Al discloses substituted diphenyl indanone, indane and indole compounds and analogues thereof useful for the treatment or prevention of diseases characterized by abnormal cell proliferation.

WO 05/07107 Al discloses diphenyl ox-indol-2-one compounds and their use in the treatment of cancer. It is generally suggested that the compounds may be present as prodrugs.

However, there is still a need for improved prodrugs of diphenyl ox-indol-2-one- type compounds.

BRIEF DESCRIPTION OF THE INVENTION

Following further developments within the field of diphenyl ox-indol-2-one compounds, the present inventors have now found that the 4-position of the phenyl moieties and the N-position of the indole represent very useful handles for the introduction of prodrug groups and that particular prodrug groups, in particular those comprising an amino acid moiety, are particularly promising.

Hence, the present invention provides compounds of the general formulae (I) and (Ia) and (Ib), cf. claims 1, 25, 28 and 29.

The present invention further provides a pharmaceutical composition, cf. claim 35, the utilization of compounds of the general formulae (I) and (Ia) and (Ib) in medicine, cf. claims 37, 38 and 40.

DETAILED DESCRIPTION OF THE INVENTION

The Compounds of the general formula (I)

The present invention La. relates to particular prodrug compounds which are useful for the treatment of cancer in a mammal.

The useful prodrug compounds have the general formula (I), namely

wherein

each of X ¹ and X ² independently

represents a prodrug group of any of the types (i)-(vi)

(i) -O-C(=O)-Z, wherein Z is selected from substituted Ci _-6 -alkyl and - CH(R ⁶ )N(R ⁷ )R ⁸ ;

(ii) -O-C(=O)-O-Y, wherein Y is selected from optionally substituted C _1-6 -alkyl, or -O-Y

R ⁷

I _R 8 represents °τ \

I

R ⁶

I I ! I

(iv) O

/

wherein A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene,

B is selected from a single bond, -O- and -S-,

R ⁵ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 - alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 -alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted

with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-β-alkylcarbonylamino, Ci _-6 -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or -C(=O)-B-R ⁵ in prodrug groups (iii) and (vii) may represent an optionally N-substituted amino acid;

R ⁶ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted C _2-6 -alkenyl, aryl, heterocyclyl, and heteroaryl, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ⁷ and R ⁸ are independently selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 - alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci-6-alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, C _1-6 - alkylsulphonyl, Ci- ₆ -alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylamino- carbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

or R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form a heterocyclic ring; and

R ⁹ is selected from hydrogen, hydroxy, optionally substituted Ci _-6 - alkyl, optionally substituted Ci _-6 -alkoxy, and optionally substituted C _2- 6-alkenyloxy;

R ¹⁰ is selected from hydroxy, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 -

alkenyloxy; aryloxy, heterocyclyloxy, and heteroaryloxy, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

provided that R ⁹ and R ¹⁰ are not both selected from hydroxy and Ci _-6 - alkoxy;

or is selected from hydrogen, hydroxy, optionally substituted Ci _-6 alkoxy, optionally substituted Ci _-6 alkyl, optionally substituted C _2-6 alkenyl, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, Ci _-6 -alkylcarbonyloxy, optionally substituted Ci _-6 alkylcarbonyl, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, mono- and di(Ci _-6 -alkyl)- aminocarbonylamino, carbamoyl, mono-and di (Ci _-6 -alkyl)aminocarbonyl, mercapto, optionally substituted Ci _-6 -alkylthio, Ci _-6 -alkylsulfonyl, mono- and di(Ci _-6 -alkyl)aminosulfonyl, cyano, halogen, aryl, aryloxy, arylamino, arylcarbonyl, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocycylcarbonyl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, where any C _1-6 -alkyl as an amino substituent is optionally substituted with hydroxyl, Ci _-6 -alkoxy, amino, mono and di (Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonyl- amino, Ci _-6 alkylaminocarbonyl or halogen(s) and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ^N

represents a prodrug group of any of the types (vii)-(viii)

wherein A, B and R ⁵ are as defined above for prodrug group (iii);

or is selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)amino- carbonyl, amino, Ci _-6 -alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 - alkylsulphonyl, and C _1-6 -alkylsulphinyl; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci- ₆ -alkylcarbonylamino, Ci _-6 -alkylamino- carbonyl, or halogen(s);

with the proviso that the compound comprises at least one of the prodrug groups (i)-(viii);

V ¹ , V ² , V ³ , and V ⁴ independently are selected from a carbon atom, a non- quaternary nitrogen atom, an oxygen atom, and a sulfur atom, and where V ⁴ further may be selected from a bond, so that -V ¹ -V ² -V ³ -V ⁴ - together with the atoms to which V ¹ and V ⁴ are attached form an aromatic or heteroaromatic ring;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a carbon atom, independently are selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted C _2-6 - alkenyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 - alkenyloxy, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, optionally substituted Ci _-6 -alkylcarbonyloxy, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, carbamoyl, mono- and di(Ci _-6 - alkyl)aminocarbonyl, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, cyano, carbamido, mono- and di(Ci _-6 -alkyl)aminocarbonylamino, Ci _-6 -alkanoyloxy, Ci _-6 - alkylsulphonyl, Ci _-6 -alkylsulphinyl, aminosulfonyl, mono- and di(Ci _-6 - alkyl)aminosulfonyl, nitro, optionally substituted Ci _-6 -alkylthio, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylcarbonyl, heteroaryl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, and halogen, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 - alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a nitrogen atom, independently are selected from hydrogen, optionally substituted d _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci-6-alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 - alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylsulphonyl, Ci _-6 - alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci- ₆ -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, C _1-6 - alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

or R ¹ and R ² together with the carbon atoms to which they are attached form a ring, e.g. an aromatic ring, a carbocyclic ring, a heterocyclic ring or a heteroaromatic ring, in particular an aromatic ring, a heterocyclic ring or a heteroaromatic ring;

with the proviso that when each of V ¹ , V ² , V ³ and V ⁴ represents a carbon atom, then R ^N , R ¹ , R ² , R ³ , and R ⁴ are not all hydrogen; and

pharmaceutically acceptable salts thereof.

Definitions

In the present context, the term "Ci _-6 -alkyl" is intended to mean a linear, cyclic or branched hydrocarbon group having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, /so-propyl, pentyl, cyclopentyl, hexyl, cyclohexyl, and the term "Ci-4-alkyl" is intended to cover linear, cyclic or branched hydrocarbon groups having 1 to 4 carbon atoms, e.g. methyl, ethyl, propyl, /so-propyl, cyclopropyl, butyl, /so-butyl, te/t-butyl, cyclobutyl.

Similarly, the term "C ₂ - ₆ -alkenyl" is intended to cover linear, cyclic or branched hydrocarbon groups having 2 to 6 carbon atoms and comprising one unsaturated

bond. Examples of alkenyl groups are vinyl, allyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, heptadecaenyl. Preferred examples of alkenyl are vinyl, allyl, butenyl, especially allyl.

In the present context, i.e. in connection with the terms "alkyl", "alkylidene", "alkoxy", "alkenyl" and the like, the term "optionally substituted" is intended to mean that the group in question may be substituted one or several times, preferably 1-3 times, with group(s) selected from hydroxy (which when bound to an unsaturated carbon atom may be present in the tautomeric keto form), Ci _-6 - alkoxy {i.e. Ci _-6 -alkyl-oxy), C _2-6 -alkenyloxy, carboxy, oxo (forming a keto or aldehyde functionality), Ci _-6 -alkoxycarbonyl, Ci _-6 -alkylcarbonyl, formyl, aryl, aryloxy, arylamino, arylcarbonyl, aryloxycarbonyl, arylcarbonyloxy, arylaminocarbonyl, arylcarbonylamino, heteroaryl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, heteroaryloxycarbonyl, heteroarylcarbonyloxy, heteroarylaminocarbonyl, heteroarylcarbonylamino, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylcarbonyl, heterocyclyloxycarbonyl, heterocyclylcarbonyloxy, heterocyclylaminocarbonyl, heterocyclylcarbonylamino, amino, mono- and di(Ci _-6 -alkyl)amino, -N(Ci _-4 - alkyl) ₃ ⁺ , carbamoyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, Ci _-6 -alkylcarbony- lamino, cyano, guanidino, carbamido, Ci _-6 -alkyl-sulphonyl-amino, aryl- sulphonyl-amino, heteroaryl-sulphonyl-amino, C _1-6 -alkanoyloxy, Ci- ₆ -alkyl- sulphonyl, d _-6 -alkyl-sulphinyl, Ci _-6 -alkylsulphonyloxy, nitro, Ci _-6 -alkylthio, and halogen, where any aryl, heteroaryl and heterocyclyl may be substituted as specifically described below for aryl, heteroaryl and heterocyclyl, and any alkyl, alkoxy, and the like, representing substituents may be substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbony- lamino, C _1-6 -alkylaminocarbonyl, or halogen(s).

Typically, the substituents are selected from hydroxy (which when bound to an unsaturated carbon atom may be present in the tautomeric keto form), Ci _-6 - alkoxy (i.e. Ci _-6 -alkyl-oxy), C _2- 6-alkenyloxy, carboxy, oxo (forming a keto or aldehyde functionality), Ci _-6 -alkylcarbonyl, formyl, aryl, aryloxy, arylamino, arylcarbonyl, heteroaryl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylcarbonyl, amino,

mono- and di(Ci _-6 -alkyl)amino; carbamoyl, mono- and di(Ci _-6 -alkyl)amino- carbonyl, amino-Ci-β-alkyl-aminocarbonyl, mono- and di(Ci-6-alkyl)amino-Ci _-6 - alkyl-aminocarbonyl, Ci-β-alkylcarbonylamino, guanidino, carbamido, Ci-6-alkyl- sulphonyl-amino, Ci _-6 -alkyl-sulphonyl, Ci _-6 -alkyl-sulphinyl, Ci _-6 -alkylthio, halogen, where any aryl, heteroaryl and heterocyclyl may be substituted as specifically described below for aryl, heteroaryl and heterocyclyl.

In some embodiments, substituents are selected from hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci- ₆ -alkylcarbonylamino, Ci _-6 - alkylaminocarbonyl, or halogen.

The term "halogen" includes fluoro, chloro, bromo, and iodo.

In the present context, the term "aryl" is intended to mean a fully or partially aromatic carbocyclic ring or ring system, such as phenyl, naphthyl, 1,2,3,4- tetrahydronaphthyl, anthracyl, phenanthracyl, pyrenyl, benzopyrenyl, fluorenyl and xanthenyl, among which phenyl is a preferred example.

The term "heteroaryl" is intended to mean a fully or partially aromatic carbocyclic ring or ring system where one or more of the carbon atoms have been replaced with heteroatoms, e.g. nitrogen ( = N- or -NH-), sulphur, and/or oxygen atoms. Examples of such heteroaryl groups are oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrrolyl, imidazolyl, pyrazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, coumaryl, furanyl, thienyl, quinolyl, benzo- thiazolyl, benzotriazolyl, benzodiazolyl, benzooxozolyl, phthalazinyl, phthalanyl, triazolyl, tetrazolyl, isoquinolyl, acridinyl, carbazolyl, dibenzazepinyl, indolyl, benzopyrazolyl, phenoxazonyl. Particularly interesting heteroaryl groups are benzimidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrrolyl, imidazolyl, pyrazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, furyl, thienyl, quinolyl, triazolyl, tetrazolyl, isoquinolyl, indolyl in particular benzimidazolyl, pyrrolyl, imidazolyl, pyridinyl, pyrimidinyl, furyl, thienyl, quinolyl, tetrazolyl, and isoquinolyl.

The term "heterocyclyl" is intended to mean a non-aromatic carbocyclic ring or ring system where one or more of the carbon atoms have been replaced with heteroatoms, e.g. nitrogen (=N- or -NH-), sulphur, and/or oxygen atoms. Examples of such heterocyclyl groups (named according to the rings) are imidazolidine, piperazine, hexahydropyridazine, hexahydropyrimidine, diazepane, diazocane, pyrrolidine, piperidine, azepane, azocane, aziridine, azirine, azetidine, pyroline, tropane, oxazinane (morpholine), azepine, dihydroazepine, tetrahydroazepine, and hexahydroazepine, oxazolane, oxazepane, oxazocane, thiazolane, thiazinane, thiazepane, thiazocane, oxazetane, diazetane, thiazetane, tetrahydrofuran, tetrahydropyran, oxepane, tetrahydrothiophene, tetrahydrothiopyrane, thiepane, dithiane, dithiepane, dioxane, dioxepane, oxathiane, oxathiepane. The most interesting examples are tetrahydrofuran, imidazolidine, piperazine, hexahydropyridazine, hexahydropyrimidine, diazepane, diazocane, pyrrolidine, piperidine, azepane, azocane, azetidine, tropane, oxazinane (morpholine), oxazolane, oxazepane, thiazolane, thiazinane, and thiazepane, in particular tetrahydrofuran, imidazolidine, piperazine, hexahydropyridazine, hexahydropyrimidine, diazepane, pyrrolidine, piperidine, azepane, oxazinane (morpholine), and thiazinane.

In the present context, i.e. in connection with the terms "aryl", "benzylidene", "heteroaryl", "heterocyclyl" and the like (e.g. "aryloxy", "heterarylcarbonyl", etc.), the term "optionally substituted" is intended to mean that the group in question may be substituted one or several times, preferably 1-5 times, in particular 1-3 times, with group(s) selected from hydroxy (which when present in an enol system may be represented in the tautomeric keto form), Ci _-6 -alkyl, Ci-6-alkoxy, C _2- 6-alkenyloxy, oxo (which may be represented in the tautomeric enol form), carboxy, Ci _-6 -alkoxycarbonyl, Ci _-6 -alkylcarbonyl, formyl, aryl, aryloxy, arylamino, aryloxycarbonyl, arylcarbonyl, heteroaryl, heteroarylamino, amino, mono- and di(Ci _-6 -alkyl)amino; carbamoyl, mono- and di(Ci _-6 -alkyl)- aminocarbonyl, amino-Ci-e-alkyl-aminocarbonyl, mono- and di(Ci _-6 -alkyl)amino- Ci-6-alkyl-aminocarbonyl, Ci-β-alkylcarbonylamino, cyano, guanidino, carbamido, Ci-6-alkanoyloxy, C _1-6 -alkyl-sulphonyl-amino, aryl-sulphonyl-amino, heteroaryl- sulphonyl-amino, C _1-6 -alkyl-suphonyl, Ci _-6 -alkyl-sulphinyl, Ci.β-alkylsulphonyl-

oxy, nitro, sulphanyl, amino, amino-sulfonyl, mono- and di(Ci _-6 -alkyl)amino- sulfonyl, dihalogen-Ci _-4 -alkyl, trihalogen-Ci _-4 -alkyl, halogen, where aryl and heteroaryl representing substituents may be substituted 1-3 times with Ci _-4 - alkyl, Ci _-4 -alkoxy, nitro, cyano, amino or halogen, and any alkyl, alkoxy, and the like, representing substituents may be substituted with hydroxy, Ci _-6 -alkoxy, C ₂ - ₆ -alkenyloxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbony- lamino, halogen, Ci _-6 -alkylthio, Ci _-6 -alkyl-sulphonyl-amino, or guanidino.

Typically, the substituents are selected from hydroxy, C _1-6 -alkyl, Ci- ₆ -alkoxy, oxo (which may be represented in the tautomeric enol form), carboxy, Ci _-6 - alkylcarbonyl, formyl, amino, mono- and di(d _-6 -alkyl)amino; carbamoyl, mono- and diCCi-e-alkylJaminocarbonyl, amino-Ci- ₆ -alkyl-aminocarbonyl, Ci _-6 - alkylcarbonylamino, guanidino, carbamido, Ci _-6 -alkyl-sulphonyl-amino, aryl- sulphonyl-amino, heteroaryl-sulphonyl-amino, C _1-6 -alkyl-suphonyl, Ci _-6 -alkyl- sulphinyl, Ci _-6 -alkylsulphonyloxy, sulphanyl, amino, amino-sulfonyl, mono- and di(Ci _-6 -alkyl)amino-sulfonyl or halogen, where any alkyl, alkoxy and the like, representing substituents may be substituted with hydroxy, Ci _-6 -alkoxy, C _2-6 - alkenyloxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbony- lamino, halogen, Ci- ₆ -alkylthio, Ci _-6 -alkyl-sulphonyl-amino, or guanidino. In some embodiments, the substituents are selected from Ci _-6 -alkyl, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, sulphanyl, carboxy or halogen, where any alkyl, alkoxy and the like, representing substituents may be substituted with hydroxy, Ci- ₆ -alkoxy, C _2-6 -alkenyloxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, halogen, Ci _-6 -alkylthio, Ci _-6 -alkyl-sulphonyl- amino, or guanidino.

The expression "optionally N-substituted amino acid" refers to an amino acid moiety wherein the α-nitrogen is represented by -N(R ⁷ )R ⁸ , wherein R ⁷ and R ⁸ are as defined herein. A non-substituted variant is the one where R ⁷ and R ⁸ are both hydrogen.

The term "prodrug" used herein is intended to mean a compound which - upon exposure to physiological conditions - will liberate a derivative said compound which then will be able to exhibit the desired biological action.

The term "pharmaceutically acceptable salts" is intended to include acid addition salts and basic salts. Illustrative examples of acid addition salts are pharmaceutically acceptable salts formed with non-toxic acids. Exemplary of such organic salts are those with maleic, fumaric, benzoic, ascorbic, succinic, oxalic, bis-methylenesalicylic, methanesulfonic, ethanedisulfonic, acetic, propionic, tartaric, salicylic, citric, gluconic, lactic, malic, mandelic, cinnamic, citraconic, aspartic, stearic, palmitic, itaconic, glycolic, p-aminobenzoic, glutamic, benzenesulfonic, and theophylline acetic acids, as well as the 8-halotheophyllines, for example 8-bromotheophylline. Exemplary of such inorganic salts are those with hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, and nitric acids. Examples of basic salts are salts where the (remaining) counter ion is selected from alkali metals, such as sodium and potassium, alkaline earth metals, such as calcium, and ammonium ions C ⁺ N(R) ₃ R ¹ , where R and R ¹ independently designates optionally substituted C _x-6 - alkyl, optionally substituted C _2-6 -alkenyl, optionally substituted aryl, or optionally substituted heteroaryl). Pharmaceutically acceptable salts are, e.g., those described in Remington's Pharmaceutical Sciences, 17. Ed. Alfonso R. Gennaro (Ed.), Mack Publishing Company, Easton, PA, U.S.A., 1985 and more recent editions and in Encyclopedia of Pharmaceutical Technology. Thus, the term "an acid addition salt or a basic salt thereof used herein is intended to comprise such salts. Furthermore, the compounds as well as any intermediates or starting materials may also be present in hydrate form.

Moreover, it should be understood that the compounds may be present as enantiomers or diastereomers, e.g. when X ¹ and X ² are different. The present invention encompasses each and every of such possible enantiomers and diastereomers as well as racemates and mixtures enriched with respect to one or the possible enantiomers or diastereomers.

Embodiments

It should be understood that the compound of the general formula (I) must include at least one prodrug group of any of the types (i), (ii), (iii), (iv), (v), (vi),

(vii) and (viii). The compound may comprise only one prodrug group, i.e. one of X ¹ and X ² is a prodrug group of any of the types (i)-(vi), or R ^N is a prodrug group of any of the types (vii)-(viii). Alternatively, the compound may comprise more than one prodrug group, e.g. both of X ¹ and X ² are prodrug group of any of the types (i)-(vi), or R ^N is a prodrug group of any of the types (vii)-(viii) while one of X ¹ and X ² is a prodrug group of any of the types (i)-(vi), or both of X ¹ and X ² are prodrug group of any of the types (i)-(vi) and R ^N is a prodrug group of any of the types (vii)-(viii).

In the currently most interesting embodiment, at least one of X ¹ and X ² represents a prodrug group (i) -O-C(=O)-Z, wherein Z is selected from substituted Ci _-6 -alkyl and -CH(R ⁶ )N(R ⁷ )R ⁸ .

In one variant hereof, Z represents a substituted Ci _-6 -alkyl.

In another currently more preferred variant, Z represents -CH(R ⁶ )-N(R ⁷ )R ⁸ .

Typically, R ⁶ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted C _2-6 -alkenyl, aryl, heterocyclyl, and heteroaryl, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted, or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a heterocyclic ring.

Also typically, R ⁷ and R ⁸ are independently selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 -alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylsulphonyl, Ci _-6 -alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci-6-alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci-6-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or

R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form a heterocyclic ring.

Within this variant, however, R ⁶ is preferably selected from side chains of essential amino acids, or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a heterocyclic ring. In such instances, R ⁶ is preferably selected from hydrogen (representing glycine), methyl (alanine), 2-propyl (valine), 2-methyl-l-propyl (leucine), 2-butyl (isoleucine), methylthioethyl (methionine), benzyl (phenylalanine), 3- indolylmethyl (tryptophan), hydroxymethyl (serine), 1-hydroxyethyl (threonine), mercaptomethyl (cysteine), 4-hydroxybenzyl (tyrosine), aminocarbonylmethyl (asparagine), 2-aminocarbonylethyl (glutamine), carboxymethyl (aspartic acid), 2-carboxyethyl (glutamic acid), 4-amino-l-butyl (lysine), 3-guanidino-l-propyl (arginine), and 4-imidazolylmethyl (histidine), or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a pyrrolidine ring (proline).

In another preferred embodiment, at least one of X ¹ and X ² represents a prodrug group (ii) -O-C(=O)-O-Y, wherein Y is selected from optionally substituted Ci-6- alkyl, or -O-Y

Also preferred are the variants of the above-mentioned embodiment (prodrug groups (i) and (ii)) where R ⁷ is hydrogen and R ⁸ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -all<oxycarbonyl, optionally substituted Ci-6-alkylcarbonyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, Ci-e-alkylsulphonyl, C _1-6 -alkylsulphinyl, aryl, arylcarbonyl, heterocyclyl, heterocyclyloxy, heteroaryl, and heteroaryloxy; where any d _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-e-alkylcarbonylamino, C _1-6 -

alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form a heterocyclic ring.

In another preferred embodiment, at least one of X ¹ and X ² represents a prodrug group (iii)

Typically, A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene; B is selected from a single bond, -O- and -S-; and R ⁵ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted Ci- ₆ -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci- ₆ -alkylcarbonyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 - alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci-β-alkoxy, amino, mono- and di(Ci-6-alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or -C(=O)-B-R ⁵ in prodrug group (iii) may represent an optionally N-substituted amino acid.

In one variant, R ⁵ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, mono- and di(d _-6 -alkyl)aminocarbonyl, amino, Ci-β-alkylcarbonylamino, mono- and di(d _-6 -alkyl)amino, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, d _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino,

carboxy, Ci-β-alkylcarbonylamino, Ci-β-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted.

In another currently preferred variant, -C(=O)-B-R ⁵ in prodrug group (iii) may represent an optionally N-substituted amino acid, i.e. B is a single bond and R ⁵ represents -CH(R ⁶ )-N(R ⁷ )R ⁸ , wherein R ⁶ , R ⁷ and R ⁸ are as defined above for prodrug groups (i) and (ii). In particular, R ⁶ is preferably selected from side chains of essential amino acids, or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a pyrrolidine ring (see the examples above for prodrug groups (i) and (ii)).

In still another embodiment, at least one of X ¹ and X ² represents a prodrug group of any of the types (iv)-(vi)

Typically, A is selected from optionally substituted d _-6 -alkylidene and optionally substituted benzylidene; R ⁹ is selected from hydrogen, hydroxy, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, and optionally substituted C _2- 6-alkenyloxy; and R ¹⁰ is selected from hydroxy, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 -alkenyloxy; aryloxy, heterocyclyloxy, and heteroaryloxy, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; provided that R ⁹ and R ¹⁰ are not both selected from hydroxy and Ci _-6 -alkoxy.

Within this embodiment, R ⁹ is preferably selected from hydrogen and hydroxy, and R ¹⁰ is preferably selected from optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 -alkenyloxy; aryloxy, heterocyclyloxy, and heteroaryloxy, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted. More particular, R ⁹ is hydroxy, and R ¹⁰ is selected from optionally substituted Ci- ₆ -alkoxy, aryloxy, heterocyclyloxy, and heteroaryloxy, wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted.

In yet another embodiment, R ^N represents a prodrug group or any of the types (vii)-(viii)

Typically, A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene; B is selected from a single bond, -O-, and -S-; and R ⁵ is selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted d _-6 -alkoxycarbonyl, optionally substituted Ci-β-alkylcarbonyl, mono- and dKCi-β-alkyOaminocarbonyl, amino, Ci-6-alkylcarbonylamino, mono- and di(d _-6 -alkyl)amino, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, C^e-alkylcarbonylamino, Cj.-6-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or -C(=O)-B-R ⁵ in prodrug group (vii) may represent an optionally N-substituted amino acid.

In a currently preferred variant, -C(=O)-B-R ⁵ in prodrug group (vii) represent an optionally N-substituted amino acid, i.e. B is a single bond and R ⁵ represents -CH(R ⁶ )-N(R ⁷ )R ⁸ , wherein R ⁶ , R ⁷ and R ⁸ are as defined above for prodrug groups (i) and (ii). In particular, R ⁶ is preferably selected from side chains of essential amino acids, or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a pyrrolidine ring (see the examples above for prodrug groups (i) and (ii)).

The function of V ¹ , V ² , V ³ , and V ⁴ is mainly believed to be of sterical character, i.e. determinative for the orientation of the groups R ^x -R ⁴ . It is, however, also believed that the selection of a heteroatom as one or more of V ¹ , V ² , V ³ , and V ⁴ may create dipole interactions with other entities and thereby have influence on, e.g., the solubility of the compounds of the general formula (I).

V ¹ , V ² , V ³ , and V ⁴ are independently selected from a carbon atom, a non- quaternary nitrogen atom, an oxygen atom, and a sulfur atom, and where V ⁴ further may be selected from a bond, so that -V ¹ -V ² -V ³ -V ⁴ - together with the atoms to which V ¹ and V ⁴ are attached form an aromatic or heteroaromatic ring. Particularly useful examples of such aromatic rings and heteroaromatic rings are those selected from a benzene ring, a thiophene ring (N^=S, V ² =V ³ =C(-) and V ⁴ =bond; V ² =S, V ¹ =V ³ =C(-) and V ⁴ =bond; or V ³ =S, V ¹ =V ² =C(-) and V ⁴ =bond), a furan ring (V^O, V ² =V ³ =C(-) and V ⁴ =bond; V ² =O, V ¹ =V ³ =C(-) and V ⁴ =bond; or V ³ =O, V ¹ =V ² =C(-) and V ⁴ =bond), a pyrazole ring (V ¹ =!^-), V ² =N, V ³ =C(-) and V ⁴ =bond; V*=N, V ² =N(-), V ³ =C(-) and V ⁴ =bond), an imidazole ring (V ^X =N(- ), V ² =C(-), V ³ =N and V ⁴ =bond; V ^X =N, V ² =C(-), V ³ =N(-) and V ⁴ =bond), a pyridine ring (V^N, V ² =V ³ =V ⁴ =C(-); V ² =N, V ¹ =V ³ =V ⁴ =C(-); V ³ =N, V ¹ =V ² =V ⁴ =C(-) and V ⁴ =N, V ¹ =V ² =V ³ =C(-)), a pyrimidine ring (V ^X =V ³ =N, V ² =V ⁴ =C(-); V ² =V ⁴ =N, V ¹ =V ³ =C(-)), pyrazines (V ¹ =V ⁴ =N, V ² =V ³ =C(-)), a pyridazine ring (V ^: =V ² =N, V ³ =V ⁴ =C(-); V ² =V ³ =N, V ¹ =V ⁴ =C(-); V ³ =V ⁴ =N, V ¹ =V ² =C(-)), a thiazole ring (V^N, V ² =C(-), V ³ =S, V ⁴ =bond; V^S, V ² =C(-), V ³ =N, V ⁴ =bond), and an isothiazole ring (V ^X =N, V ² =S, V ³ =C(-), V ⁴ =bond; V ^X =S, V ² =N, V ³ =C(-), V ⁴ =bond; V ¹ =^-), V ² =S, V ³ =N, V ⁴ =bond; V ¹ =C(-), V ² =N, V ³ =S, V ⁴ =bond).

The meaning of V ¹ , V ² , V ³ and V ⁴ for each heteroaromatic ring is merely specified for the purpose of illustrating that various orientations of the heteroatoms are possible. Furthermore, it should be understood that the respective rings carry the substituents R ¹ , R ² , R ³ and R ⁴ (where applicable) in accordance with the general formula (I). Thus, specification of "C(-)" and "N(-)" as possible meanings of V ¹ , V ² , V ³ and V ⁴ is made for the purpose of describing that the atoms in question carry a substituent (which may be hydrogen). Specification of "N" means that the respective atoms do not carry an "R" substituent, i.e. the corresponding "R" substituent is absent.

In one embodiment, -V ¹ -V ² -V ³ -V ⁴ - together with the atoms to which V ¹ and V ⁴ are attached form a ring selected from a benzene ring, a thiophene ring, a furan ring, a pyrazole ring, an imidazole ring, a pyridine ring, a pyrimidine ring, pyrazines, and a pyridazine ring, in particular from a benzene ring and a pyridine ring where the nitrogen atom represents V ³ (see also the Examples). In accordance with the general formula (I), the respective ring (aromatic or heteroaromatic) carries the substituents R^R ⁴ (where applicable).

The substituents R*-R ⁴ (where applicable) are believed to be at least partly responsible for the biological effect, e.g. the ability of the compounds to inhibit cell proliferation in cancer cells.

In one embodiment, R ¹ , R ² , R ³ , and R ⁴ are, when attached to a carbon atom, independently selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted C _2-6 -alkenyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2- 6-alkenyloxy, carboxy, optionally substituted Ci _-6 - alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, optionally substituted Ci-6-alkylcarbonyloxy, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, carbamoyl, mono- and di(C _1-6 -alkyl)aminocarbonyl, Ci _-6 -alkylcarbonylamino, Ci _-6 - alkylsulphonylamino, cyano, carbamido, mono- and di(Ci- ₆ -alkyl)amino- carbonylamino, Ci _-6 -alkanoyloxy, Ci _-6 -alkylsulphonyl, Ci _-6 -alkylsulphinyl, aminosulfonyl, mono- and di(Ci _-6 -alkyl)aminosulfonyl, nitro, optionally substituted Ci _-6 -alkylthio, and halogen, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono-

and di(Ci _-6 -alkyl)amino, carboxy, Ci.e-alkylcarbonylamino, Ci _-6 -alkylamino- carbonyl, or halogen(s); and R ¹ , R ² , R ³ , and R ⁴ are, when attached to a nitrogen atom, independently selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _x-6 - alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and diCCi-e-alkyOaminocarbonyl, amino, Ci-β-alkylcarbonylamino, mono- and di(Ci _-6 - alkyl)amino, Ci _-6 -alkylsulphonyl, and Ci _-6 -alkylsulphinyl; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-β-alkylcarbonylamino, Ci _-6 - alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted.

More particularly, R ¹ , R ² , R ³ , and R ⁴ are independently selected from hydrogen, halogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 - alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 - alkylcarbonyl, amino, Ci _-6 -alkylcarbonylamino, Ci-β-alkylcarbonylamino, Ci _-6 - alkylsulphonylamino, mono- and di(Ci _-6 -alkyl)aminosulfonyl, and mono- and di(Ci _-6 -alkyl)amino, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, d-β-alkylcarbonylamino, Ci-e-alkylaminocarbonyl, or halogen(s), such as from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, amino, Ci-β-alkylcarbonylamino, Ci _-6 -alkyl- carbonylamino, Ci _-6 -alkylsulphonylamino, mono- and di(Ci _-6 -alkyl)aminosulfonyl, and mono- and di(Ci _-6 -alkyl)amino, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 - alkyl)amino, carboxy, Ci-β-alkylcarbonylamino, Ci-β-alkylaminocarbonyl, or halogen(s).

As an alternative to the above, R ¹ and R ² may in one embodiment together with the carbon atoms to which they are attached form a heterocyclic ring or a heteroaromatic ring; and in another embodiment, R ¹ and R ² may together with the carbon atoms to which they are attached form an aromatic ring or a carbocyclic ring.

Preferably, R ¹ , R ² , R ³ and R ⁴ are not all hydrogen.

In a currently highly preferred embodiment, R ¹ and R ² are both halogen, in particular, R ¹ and R ² are both fluoro. In a variant within this embodiment, R ^N , R ³ and R ⁴ are all hydrogen.

It is believed that R ^N may be selected from a wide variety of substituents including the prodrug group (vi). If not being a prodrug group, R ^N may advantageous be selected from hydrogen, Ci _-6 -alkyl, amino, and Ci _-6 - alkylcarbonylamino. Most preferred is the variants wherein R ^N is selected from hydrogen and Ci _-6 -alkyl, in particular from hydrogen and methyl, most typical hydrogen.

In one currently preferred variant,

each of V ¹ , V ² , V ³ , and V ⁴ represents a carbon atom;

R ¹ and R ² are both fluoro;

R ³ and R ⁴ are all hydrogen;

R ^N is hydrogen;

at least one of X ¹ and X ² represents a prodrug group (i) -O-C(=O)-CH(R ⁶ )- N(R ⁷ )R ⁸ ,

wherein R ⁶ is selected from hydrogen (glycine), methyl (alanine), 2-propyl (valine), 2-methyl-l-propyl (leucine), 2-butyl (isoleucine), methylthioethyl (methionine), benzyl (phenylalanine), 3-indolylmethyl (tryptophan), hydroxymethyl (serine), 1-hydroxyethyl (threonine), mercaptomethyl (cysteine), 4-hydroxybenzyl (tyrosine), aminocarbonylmethyl (asparagine), 2- aminocarbonylethyl (glutamine), carboxymethyl (aspartic acid), 2-carboxyethyl (glutamic acid), 4-amino-l-butyl (lysine), 3-guanidino-l-propyl (arginine), and

4-imidazolylmethyl (histidine), or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a pyrrolidine ring (proline);

R ⁷ is hydrogen and R ⁸ is selected from hydrogen, optionally substituted Ci _-6 - alkyl, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted C _x-6 - alkylcarbonyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, d _-6 -alkylsulphonyl, Ci _-6 - alkylsulphinyl, aryl, arylcarbonyl, heterocyclyl, heterocyclyloxy, heteroaryl, and heteroaryloxy; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-e-alkylcarbonylamino, Ci-e-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form a heterocyclic ring; and

any other of X ¹ or X ² is selected from hydrogen, hydroxy, optionally substituted Ci _-6 alkoxy, optionally substituted Ci _-6 alkyl, optionally substituted C _2-6 alkenyl, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, Ci _-6 -alkylcarbonyloxy, optionally substituted Ci _-6 alkylcarbonyl, formyl, amino, mono- and di(Ci _-6 - alkyl)amino, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, mono- and di(Ci _-6 -alkyl)aminocarbonylamino, carbamoyl, mono-and di (C _1-6 -alkyl)- aminocarbonyl, mercapto, optionally substituted Ci _-6 -alkylthio, Ci _-6 -alkylsulfonyl, mono- and cyano, halogen, aryl, aryloxy, arylamino, arylcarbonyl, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocycylcarbonyl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxyl, Ci _-6 -alkoxy, amino, mono and di (Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonyl- amino, Ci _-6 alkylaminocarbonyl or halogen(s) and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted.

In one particular embodiment, R ¹ is selected from hydrogen, halogen, Ci _-6 -alkyl, trifluoromethyl and Ci _-6 -alkoxy, when V ¹ is a carbon atom.

In a further embodiment, R ² is selected from hydrogen, halogen, optionally substituted aryl, optionally substituted aryloxy, and optionally substituted heteroaryl, when V ² is a carbon atom.

In a still further embodiment, R ³ is selected from hydrogen, optionally substituted Ci- ₆ -alkoxy, halogen, cyano, optionally substituted aryl, optionally substituted aryloxy, optionally substituted heteroaryl, amino, Ci _-6 -alkylcarbony- lamino, Ci _-6 -alkylsulphonylamino, and mono- and di(Ci _-6 -alkyl)aminosulfonyl, when V ³ is a carbon atom.

In an even still further embodiment, R ⁴ is hydrogen, when V ⁴ is a carbon atom.

The Compounds of general formula (Ia)

It has been found that the compounds wherein at least one prodrug group comprising an amino acid moiety is present represents a particularly interesting aspect of the present invention. Hence, the present invention also provides a compound of the general formula (Ia)

wherein

at least one of X ¹ , X ² and R ^N represent a prodrug group comprising an amino acid moiety,

any of X ¹ and X ² not being a prodrug group independently being selected from hydrogen, hydroxy, optionally substituted Ci _-6 alkoxy, optionally substituted Ci _-6

alkyl, optionally substituted C _2-6 alkenyl, carboxy, optionally substituted Ci _-6 - alkoxycarbonyl, Ci- ₆ -alkylcarbonyloxy, optionally substituted Ci _-6 alkylcarbonyl, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, Ci-β-alkylcarbonylamino, Ci _-6 - alkylsulphonylamino, mono- and diCCi-β-alkyOaminocarbonylamino, carbamoyl, mono-and di (Ci- ₆ -alkyl)aminocarbonyl, mercapto, optionally substituted Ci _-6 - alkylthio, Ci _-6 -alkylsulfonyl, mono- and di(Ci _-6 -alkyl)aminosulfonyl, cyano, halogen, aryl, aryloxy, arylamino, arylcarbonyl, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocycylcarbonyl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxyl, Ci _-6 -alkoxy, amino, mono and di (Ci _-6 -alkyl)amino, carboxy, Ci- ₆ -alkylcarbonylamino, d _-6 alkylaminocarbonyl or halogen(s) and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

and R ^N not being a prodrug group being selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _1-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci-e-alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylsulphonyl, and Ci _-6 -alkylsulphinyl; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 - alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-β-alkylcarbonylamino, Ci-β-alkylaminocarbonyl, or halogen(s);

V ¹ , V ² , V ³ , and V ⁴ independently are selected from a carbon atom, a non- quaternary nitrogen atom, an oxygen atom, and a sulfur atom, and where V ⁴ further may be selected from a bond, so that -V ¹ -V ² -V ³ -V ⁴ - together with the atoms to which V ¹ and V ⁴ are attached form an aromatic or heteroaromatic ring;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a carbon atom, independently are selected from hydrogen, optionally substituted Ci _-6 -alkyl, optionally substituted C _2-6 - alkenyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 - alkenyloxy, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, optionally substituted Ci _-6 -alkylcarbonyloxy, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, carbamoyl, mono- and di(Ci _-6 - alkyl)aminocarbonyl, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, cyano,

carbamido, mono- and di(Ci _-6 -alkyl)aminocarbonylamino, Ci _-6 -alkanoyloxy, Ci _-6 - alkylsulphonyl, Ci _-6 -alkylsulphinyl, aminosulfonyl, mono- and di(Ci _-6 - alkyl)aminosulfonyl, nitro, optionally substituted Ci _-6 -alkylthio, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylcarbonyl, heteroaryl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, and halogen, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci- ₆ -alkoxy, amino, mono- and di(Ci _-6 - alkyl)amino, carboxy, Ci- ₆ -alkylcarbonylamino, Ci- ₆ -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a nitrogen atom, independently are selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci- ₆ -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci- ₆ -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 - alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylsulphonyl, Ci _-6 - alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 - alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

or R ¹ and R ² together with the carbon atoms to which they are attached form a ring, e.g. an aromatic ring, a carbocyclic ring, a heterocyclic ring or a heteroaromatic ring, in particular an aromatic ring, a heterocyclic ring or a heteroaromatic ring;

with the proviso that when each of V ¹ , V ² , V ³ and V ⁴ represents a carbon atom, then R ^N , R ¹ , R ² , R ³ , and R ⁴ are not all hydrogen; and

pharmaceutically acceptable salts thereof.

In this embodiment, at least one of X ¹ and X ² is preferably a prodrug group of any of the types (ia) and (iiia)

(ia) -O-C(=O)-CH(R ⁶ )N(R ⁷ )R ⁸ ; and

' _/ O _^ A _^ CL Y _/ B^ N* ⁵ (iiia) ° ;

wherein A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene,

-C(=O)-B-R ⁵ represent an optionally N-substituted amino acid;

R ⁶ is selected from hydrogen (glycine), methyl (alanine), 2-propyl (valine), 2- methyl-1-propyl (leucine), 2-butyl (isoleucine), methylthioethyl (methionine), benzyl (phenylalanine), 3-indolylmethyl (tryptophan), hydroxymethyl (serine), 1-hydroxyethyl (threonine), mercaptomethyl (cysteine), 4-hydroxybenzyl (tyrosine), aminocarbonylmethyl (asparagine), 2-aminocarbonylethyl (glutamine), carboxymethyl (aspartic acid), 2-carboxyethyl (glutamic acid), 4- amino-1-butyl (lysine), 3-guanidino-l-propyl (arginine), and 4-imidazolylmethyl (histidine), or R ⁶ and R ⁸ together with the intervening carbon and nitrogen atoms to which they are attached form a pyrrolidine ring (proline);

R ⁷ and R ⁸ are independently selected from hydrogen, optionally substituted Ci _-6 - alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 - alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 -alkylcarbonylamino, mono- and di(Ci _-6 - alkyl)amino, d _-6 -alkylsulphonyl, Ci _-6 -alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, d _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci-β-alkylcarbonylamino, Ci-6-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

or R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form a heterocyclic ring.

Moreover, R ^N may (as the sole prodrug group or in combination with another prodrug group as X ¹ and/or X ² ) represents a prodrug group of the type (vii)

wherein A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene, and -C(=O)-B-R ⁵ represent an optionally N-substituted amino acid.

The further specifications and indications of preferred meanings for the substituents X ¹ , X ² , R ^N , V ¹ , V ² , V ³ , V ⁴ , R ¹ , R ² , R ³ _; R ⁴ , R ⁵ , R ⁶ , R ⁷ , and R ⁸ given in connection with compound of general formula (I) also apply for the compounds of general formula (Ia), mutatis mutandis.

The Compounds of general formula (Ib)

It has also been found that compounds comprising other particular prodrug groups represents a further particularly interesting aspect of the present invention. Hence, the present invention also provides a compound of the general formula (Ib)

(Ib)

wherein

each of X ¹ and X ² independently

represents a prodrug group of any of the types (ix)-(x)

(ix) -O-C(=O)-Z, wherein Z is selected from optionally substituted Ci _-6 -alkenyl and -N(R ⁷ )R ⁸ ; and

(x) -O-CH ₂ -C(=O)-Y, wherein Y is selected from optionally substituted Ci _-6 -alkyl;

wherein R ⁷ and R ⁸ are independently selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci _-6 - alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted C _1-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)amino- carbonyl, amino, Ci-β-alkylcarbonylamino, mono- and di(Ci _-6 - alkyl)amino, Ci _-6 -alkylsulphonyl, Ci _-6 -alkylsulphinyl, aryl, aryioxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylami- no, Ci-6-alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted, or R ⁷ and R ⁸ together with the nitrogen atoms to which they are attached form an optionally substituted heterocyclic ring;

or is selected from hydrogen, hydroxy, optionally substituted C _1-6 alkoxy, optionally substituted Ci _-6 alkyl, optionally substituted C _2-6 alkenyl, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, Ci _-6 -alkylcarbonyloxy, optionally substituted Ci _-6 alkylcarbonyl, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, Ci-6-alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, mono- and di(Ci _-6 -alkyl)- aminocarbonylamino, carbamoyl, mono-and di (Ci _-6 -alkyl)aminocarbonyl, mercapto, optionally substituted Ci _-6 -alkylthio, C _1-6 -alkylsulfonyl, mono- and

di(Ci _-6 -alkyl)aminosulfonyl, cyano, halogen, aryl, aryloxy, arylamino, arylcarbonyl, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocycylcarbonyl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, where any Ci-β-alkyl as an amino substituent is optionally substituted with hydroxyl, Ci- ₆ -alkoxy, amino, mono and di (Ci _-6 -alkyl)amino, carboxy, Ci-β-alkylcarbonyl- amino, Ci _-6 alkylaminocarbonyl or halogen(s) and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ^N

represents a prodrug group of any of the types (xi)-(xii)

(xi) -A-C(=O)-B-R ⁵ ; and

(xii) -(CH ₂ -CH ₂ -O) _1-10 -R ⁵ ,

wherein A is selected from optionally substituted Ci _-6 -alkylidene and optionally substituted benzylidene,

B is selected from a single bond, -O- and -S-, and

R ⁵ is selected from hydrogen, optionally substituted C _1-6 -alkyl, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci _-6 - alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 -alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci- ₆ -alkoxy, amino, mono- and di(Ci- ₆ -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted; or -C(=O)-B-R ⁵ in prodrug group (xi) may represent an optionally N-substituted amino acid;

or is selected from hydrogen, optionally substituted C _1-6 -alkyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted Ci- ₆ -alkoxycarbonyl, optionally substituted Ci _-6 -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)amino- carbonyl, amino, Ci- ₆ -alkylcarbonylamino, mono- and di(Ci-6-alkyl)amino, Ci _-6 - alkylsulphonyl, and Ci _-6 -alkylsulphinyl; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci- ₆ -alkylcarbonylamino, Ci _-6 -alkylamino- carbonyl, or halogen(s);

with the proviso that the compound comprises at least one of the prodrug groups (ix)-(xii);

V ¹ , V ² , V ³ , and V ⁴ independently are selected from a carbon atom, a non- quaternary nitrogen atom, an oxygen atom, and a sulfur atom, and where V ⁴ further may be selected from a bond, so that -V ¹ -V ² -V ³ -V ⁴ - together with the atoms to which V ¹ and V ⁴ are attached form an aromatic or heteroaromatic ring;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a carbon atom, independently are selected from hydrogen, optionally substituted C _1-6 -alkyl, optionally substituted C _2-6 - alkenyl, hydroxy, optionally substituted Ci _-6 -alkoxy, optionally substituted C _2-6 - alkenyloxy, carboxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted d _-6 -alkylcarbonyl, optionally substituted Ci _-6 -alkylcarbonyloxy, formyl, amino, mono- and di(Ci _-6 -alkyl)amino, carbamoyl, mono- and di(Ci _-6 - alkyl)aminocarbonyl, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylsulphonylamino, cyano, carbamido, mono- and diCCi-β-alkyOaminocarbonylamino, Ci _-6 -alkanoyloxy, Ci _-6 - alkylsulphonyl, Ci _-6 -alkylsulphinyl, aminosulfonyl, mono- and di(Ci _-6 - alkyl)aminosulfonyl, nitro, optionally substituted Ci _-6 -alkylthio, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylcarbonyl, heteroaryl, heteroaryloxy, heteroarylamino, heteroarylcarbonyl, and halogen, where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 - alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 -alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

R ¹ , R ² , R ³ , and R ⁴ , when attached to a nitrogen atom, independently are selected from hydrogen, optionally substituted Ci _-6 -alkyl, hydroxy, optionally substituted Ci- ₆ -alkoxy, optionally substituted Ci _-6 -alkoxycarbonyl, optionally substituted Ci- ₆ -alkylcarbonyl, formyl, mono- and di(Ci _-6 -alkyl)aminocarbonyl, amino, Ci _-6 - alkylcarbonylamino, mono- and di(Ci _-6 -alkyl)amino, Ci _-6 -alkylsulphonyl, Ci _-6 - alkylsulphinyl, aryl, aryloxy, arylcarbonyl, arylamino, heterocyclyl, heterocyclyloxy, heterocyclylcarbonyl, heterocyclylamino, heteroaryl, heteroaryloxy, heteroarylcarbonyl, and heteroarylamino; where any Ci _-6 -alkyl as an amino substituent is optionally substituted with hydroxy, Ci _-6 -alkoxy, amino, mono- and di(Ci _-6 -alkyl)amino, carboxy, Ci _-6 -alkylcarbonylamino, Ci _-6 - alkylaminocarbonyl, or halogen(s), and wherein any aryl, heterocyclyl and heteroaryl may be optionally substituted;

or R ¹ and R ² together with the carbon atoms to which they are attached form a ring, e.g. an aromatic ring, a carbocyclic ring, a heterocyclic ring or a heteroaromatic ring, in particular an aromatic ring, a heterocyclic ring or a heteroaromatic ring;

with the proviso that when each of V ¹ , V ² , V ³ and V ⁴ represents a carbon atom, then R ^N , R ¹ , R ² , R ³ , and R ⁴ are not all hydrogen; and

pharmaceutically acceptable salts thereof.

In one variant, at least one of X ¹ and X ² represents a prodrug group (ix) - O-C(=O)-Z.

In another variant, at least one of, wherein X ¹ and X ² represents a prodrug group (x) -O-CH ₂ -C(=O)-Y.

In a further variant, R ^N represents a prodrug group (xi) -A-C(=O)-B-R ⁵ .

In a still further variant, R ^N represents a prodrug group (xii) -(CH ₂ -CH ₂ -O) _1-I0 - R ⁵ .

The further specifications and indications of preferred meanings for the substituents X ¹ , X ² , R ^N , V ¹ , V ² , V ³ , V ⁴ , R ¹ , R ² , R ³ , R ⁴ , R ⁵ , R ⁷ , and R ⁸ given in connection with compound of general formula (I) also apply for the compounds of general formula (Ib), mutatis mutandis.

Currently most preferred compounds

Presently very interesting compounds of the formulae (I) and (Ia) and (Ib) are those listed in the following:

(2S)-4-(6,7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3-y l)phenyl 2- aminopropanoate hydrochloride, (2S,2'S)-4, 4'-(6,7-difluoro-2-oxoindoline-3,3-diyl)bis (4,1-phenylene) bis(2- aminopropanoate dihydrochloride,

4,4'-(6,7-difluoro-2-oxoindoline-3 _/ 3-diyl)bis (4,1-phenylene) bis(2- aminoacetate) ditrifluoroacetic acid salt,

(2S,2'S)-4,4'-(6,7-difluoro-2-oxoindoline-3,3-diyl)bis (4,1-phenylene) bis(3- methyl-2-(methylamino)butanoate) dihydrochloride,

4,4'-(6,7-difluoro-2-oxoindoline-3,3-diyl)bis (4,1-phenylene) bis(2- dimethylamino)acetate),

(2S)-4-(6,7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3-y l)phenyl 2- aminophenylpropanoate hydrochloride, 4-(3-(4-chlorophenyl)-6,7-difuoro-2-oxoindolin-3-yl)phenyl (2- morpholinoethoxy)methyl carbonate,

(7-fluoro-3,3-bis(4-hydroxyphenyl)-6-methyl-2-oxoindolin- l-yl)methyl 2- morpholinoethyl carbonate,

(4,4 ^' -(6-fluoro-7-methyl-2-oxoindoline-3,3-diyl)bis(4,l- phenylene)bis(oxy)bis(methylene) bis(2-morpholinoethyl) dicarbonate, and

4-(3-(4-chlorophenyl)-6,7-dimethyl-2-oxoindolin-3-yl)phen yl methyl methylphosphonate

Presently very interesting compounds of the formula (Ib) are those listed in the following:

6,7-Difluoro-3-(4-fluorophenyl)-l-(2-hydroxyethyl)-3-(4-hydr oxyphenyl)-l,3- dihydro-2/7-indol-2-one _;

4-[6,7-Difluoro-3-(4-fluorophenyl)-2-oxo-2,3-dihydro-lH-i ndol-3-yl]phenyl 3- morpholinopropanoate, 4-[6,7-Difluoro-3-(4-fluorophenyl)-2-oxo-2,3-dihydro-lH-indo l-3-yl]phenyl 3-(4- methylpiperazino)propanoate,

[3-(4-{[(Isopropoxycarbonyl)oxy]methoxy}phenyl)-3-(4-meth oxyphenyl)-2- oxo-2, 3-dihydro-lAV-indol-l-yl]methyl isopropyl carbonate,

Benzyl 2-[3-{4-[2-(benzyloxy)-2-oxoethoxy]phenyl}-3-(4-methoxypheny l)-2- oxo-2,3-dihydro-lH-indol-l-yl]acetate,

4-(3-{4-[(Anilinocarbonyl)oxy]phenyl}-6,7-difluoro-2-oxo- 2,3-dihydro-l/-Hndol-

3-yl)phenyl λ/-phenylcarbamate,

4-[6,7-Difluoro-3-(4-fluorophenyl)-2-oxo-2,3-dihydro-lH-i ndol-3-yl]phenyl N, N- dimethylcarbamate, 4-[6-Fluoro-3-(4-fluorophenyl)-7-methyl-2-oxo-2,3-dihydro-lH -indol-3- yl]phenyl N,N-dimethylcarbamate, and

4-[6-Fluoro-3-(4-fluorophenyl)-7-methyl-2-oxo-2,3-dihydro -lH-indol-3- yl]phenyl acrylate.

Preparation of compounds

The compounds of the present invention can be prepared in a number of ways well known to those skilled in the art of organic synthesis. The compounds of the present invention can be synthesized using the methods outline below and in the Examples section, together with methods known in the art of organic synthetic organic chemistry, or variations thereof as appreciated by those skilled in the art. Preferred methods include, but are not limited to, those described below.

The novel compounds of formulae (I) and (Ia) and (Ib) may be prepared using the reactions and techniques described in this section. The reactions are performed in solvents appropriate to the reagents and materials employed and suitable for the transformations being effected. Also, in the synthetic methods described below, it is to be understood that all proposed reaction conditions,

including choice of solvent, reaction atmosphere, reaction temperature duration of experiment and work-up procedures, are chosen to be conditions of standard for that reaction, which should be readily recognized by one skilled in the art. It is understood by one skilled in the art of organic synthesis that the functionality present on various portions of the educt molecule must be compatible with the reagents and reactions proposed. Not all molecules of formulae (I) and (Ia) and (Ib) falling into a given class may be compatible with some of the reaction conditions required in some of the methods described. Such restrictions to the substituents which are compatible with the reaction conditions will be readily apparent to one skilled in the art and alternative methods can be used.

Compounds according to the present invention in which X ¹ and/or X ² is an amino acid ester may be prepared from compounds of general formula (II) or (III) by coupling with a protected amino acid and subsequent removal of the protecting

groups, if any, to yield compounds of general formula (IV) and (V). The condensation is carried out using any of the many methods for the formation of ester bonds known to one skilled in the art of organic synthesis. These methods include, but are not limited to, use of standard coupling procedures such as use of symmetric carbonic anhydrides, mixed carbonic anhydride (e.g. isobutyl chloroformate) method, carbodiimides (e.g. N,N-dimethylaminopropyl-N'-ethyl carbodiimide, dicyclohexyl carbodiimide, diisopropyl carbodiimide), active ester (e.g. pentaflurophenyl ester, p-nitrophenyl ester, N-hydroxysuccinic imido ester) method, carbonyldiimidazole method, azide method, phosphorous reagents such as BOP-CI, conversion of the protected amino acid derivative into an acid chloride. Some of these methods (especially carbodiimide) can be enhanced by addition of e.g. 1-hydroxybenzotriazole or N,N-dimethylaminopyridine.

Protection groups as referred to above are well known per se, for example from the techniques of peptide chemistry. Amino groups can often be protected by te/t-butyloxycarbonyl, benzyloxycarbonyl or acetyl groups, or in the form of a phtalimido group. Hydroxy groups are often protected as readily cleavable ethers such as the t-butyl or benzyl ether, or as readily cleavable esters such as the acetate. Carboxylic acid groups are often protected as readily cleavable esters such as the t-butyl or benzyl ester. Thiols are often protected as readily cleavable ethers such as the trityl ether.

(VII)

If R ⁷ and R ⁸ are both alkyl groups compounds of general formula (IV) and (V) can be converted into the corresponding trialkylammonium salts (VI) and (VII), e.g. by reaction with an alkyl halide and a base or methyl methane sulfonate.

Compounds according to the present invention in which Xi and/or X ₂ is a phosphonate group or a phosphinate group (VIII) and (IX) may be prepared from compounds of general formula (II) or (III) e.g. by condensation with a phosphonochloridate or a phosphinic chloride in the presence of a base.

Compounds according to the present invention in which Xi and/or X ₂ is -O-A- 0(C=O)-B-R ⁵ (X) and (XI) can be prepared form compounds of general (II) and (III), in which R ^N is an amide protecting group (e.g. a silyl-type or benzyl type protecting group) by reaction with a base and chloromethyl or iodomethyl esters of general formula (XII), and subsequent removal of the amide protecting group (e.g. by use of fluoride ion or hydrogenation). The chloromethyl or iodomethyl esters of general formula (XII) may be prepared as described in Bioorg. Med. Chem. Lett. (2005) 13 2491-2494.

(XII)

Alternatively, compounds of general formula (X) and (XI) may prepared by similar methods to those described in Bioorg. Med. Chem. Lett. (2003) 1695-1698 after suitable protection of the amide group and subsequent removal of the protecting group, as described above.

Compounds according to the present invention in which R ^N is-O-A-O(C=O)-B-R ⁵ , Xi and/or X ₂ is -0-A-O(C=O)-B-R ⁵ (X) and (XI) can be prepared form compounds of general (II) and (III) by reaction with a base of the right choice and chloromethyl or iodomethyl esters of general formula (XII).

Compounds according to the present invention in which R ^N is-O-A-O(C=O)-B-R ⁵ , Xi and/or X ₂ is hydrogen (XII) and (XIII) can be prepared form compounds of general (II) and (III) reaction with a base of the right choice, e.g. NaH, and chloromethyl or iodomethyl esters of general formula (XII). The phenolic group(s) may require protection prior to derivatization of the amide function, e.g. with a Boc-group, a silyl ether or an acyl group, followed by deprotection as the last step.

Compounds according to the present invention in which Xi and/or X ₂ is OA- 0(C=O)-B-R ⁵ (XIV) and (XV) can be prepared form compounds of general (II) and (III) by reaction with chloromethyl carbonochloridate and a base, e.g. potassium carbonate or cesium carbonate, and subsequent reaction of the resulting chloromethyl phenylcarbonate with an alcohol or a thiol and a base. Compounds according to the present invention in which R ^N as well as Xi and/or X ₂ is -0-CO-CH ₂ -OR (XIV) and (XV) can be prepared form compounds of general (II) and (III) can be prepared as decribed above with a different choice of base, e.g. NaH.

Compounds according to the present invention in which R ^N is 0-A-O(C=O)-B-R ⁵ (XVI) and (XVII) can be prepared form compounds of general (II) and (III) by protection of the phenolic function(s), e.g. with a Boc-group, a silyl ether or an acyl group, reaction with chloromethyl carbonochloridate and a base, e.g. NaH, and subsequent reaction of the resulting chloromethyl 3,3-bis(phenyl)-2- oxoindoline-1-carboxylate with an alcohol or a thiol, and a base, followed by removal of the protecting group(s).

Medical uses

The compounds of the general formulae (I) and (Ia) and (Ib) are believed to be particularly useful in the treatment of cancer. The term cancer is typically describing cell growth not under strict control. In one embodiment of the invention, treatment of cancers in which inhibition of protein synthesis and/or inhibition of activation of the mTOR pathway is an effective method for reducing cell growth. Examples of such cancers are breast cancer, renal cancer, multiple myeloma, leukemia, glio blastoma, rhabdomyosarcoma, prostate, soft tissue sarcoma, colorectal sarcoma, gastric carcinoma, head and neck squamous cell carcinoma, uterine, cervical, melanoma, lymphoma, and pancreatic cancer.

Hence, the present invention generally provides a compound of the general formula (I) or (Ia) or (Ib) as defined herein for use as a medicament; more particular, the use of a compound of the general formula (I) or (Ia) or (Ib) as defined herein for the preparation of a medicament for the treatment of cancer in a mammal. Such medicaments may further comprise one or more other chemotherapeutic agents.

Moreover, the present invention provides a method of treating a mammal suffering from or being susceptible to cancer, the method comprising administering to the mammal a therapeutically effective amount of a compound of the general formula (I) or (Ia) or (Ib) as defined herein.

Formulation of pharmaceutical compositions

The compounds of the general formulae (I) and (Ia) and (Ib) are suitably formulated in a pharmaceutical composition so as to suit the desirable route of administration.

The administration route of the compounds may be any suitable route which leads to a concentration in the blood or tissue corresponding to a therapeutic effective concentration. Thus, e.g., the following administration routes may be applicable although the invention is not limited thereto: the oral route, the parenteral route, the cutaneous route, the nasal route, the rectal route, the vaginal route and the ocular route. It should be clear to a person skilled in the art that the administration route is dependent on the particular compound in question; particularly the choice of administration route depends on the physico- chemical properties of the compound together with the age and weight of the patient and on the particular disease or condition and the severity of the same.

The compounds may be contained in any appropriate amount in a pharmaceutical composition, and are generally contained in an amount of about 1-95%, e.g. 1-10%, by weight of the total weight of the composition. The composition may be presented in a dosage form which is suitable for the oral, parenteral, rectal, cutaneous, nasal, vaginal and/or ocular administration route.

Thus, the composition may be in form of, e.g., tablets, capsules, pills, powders, granulates, suspensions, emulsions, solutions, gels including hydrogels, pastes, ointments, creams, plasters, drenches, delivery devices, suppositories, enemas, injectables, implants, sprays, aerosols and in other suitable form.

The pharmaceutical compositions may be formulated according to conventional pharmaceutical practice, see, e.g., "Remington's Pharmaceutical Sciences" and "Encyclopedia of Pharmaceutical Technology", edited by Swarbrick, J. & J. C. Boylan, Marcel Dekker, Inc., New York, 1988. Typically, the compounds defined herein are formulated with (at least) a pharmaceutically acceptable carrier or excipient. Pharmaceutically acceptable carriers or excipients are those known by the person skilled in the art. Formation of suitable salts of the compounds of the Formulae (I) and (Ia) and (Ib) will also be evident in view of the before- mentioned.

Thus, the present invention provides in a further aspect a pharmaceutical composition comprising a compound of the general Formula (I) or (Ia) or (Ib) in combination with a pharmaceutically acceptable carrier.

Pharmaceutical compositions according to the present invention may be formulated to release the active compound substantially immediately upon administration or at any substantially predetermined time or time period after administration. The latter type of compositions is generally known as controlled release formulations.

In the present context, the term "controlled release formulation" embraces i) formulations which create a substantially constant concentration of the drug within the body over an extended period of time, ii) formulations which after a predetermined lag time create a substantially constant concentration of the drug within the body over an extended period of time, iii) formulations which sustain drug action during a predetermined time period by maintaining a relatively, constant, effective drug level in the body with concomitant minimization of undesirable side effects associated with fluctuations in the plasma level of the active drug substance (saw-tooth kinetic pattern), iv) formulations which

attempt to localize drug action by, e.g., spatial placement of a controlled release composition adjacent to or in the diseased tissue or organ, v) formulations which attempt to target drug action by using carriers or chemical derivatives to deliver the drug to a particular target cell type.

Controlled release formulations may also be denoted "sustained release", "prolonged release", "programmed release", "time release", "rate-controlled" and/or "targeted release" formulations.

Controlled release pharmaceutical compositions may be presented in any suitable dosage forms, especially in dosage forms intended for oral, parenteral, cutaneous nasal, rectal, vaginal and/or ocular administration. Examples include single or multiple unit tablet or capsule compositions, oil solutions, suspensions, emulsions, microcapsules, microspheres, nanoparticles, liposomes, delivery devices such as those intended for oral, parenteral, cutaneous, nasal, vaginal or ocular use.

Preparation of solid dosage forms for oral use, controlled release oral dosage forms, fluid liquid compositions, parenteral compositions, controlled release parenteral compositions, rectal compositions, nasal compositions, percutaneous and topical compositions, controlled release percutaneous and topical compositions, and compositions for administration to the eye will be well-known to those skilled in the art of pharmaceutical formulation. Specific formulations can be found in "Remington's Pharmaceutical Sciences".

Capsules, tablets and pills etc. may contain for example the following compounds: microcrystalline cellulose, gum or gelatin as binders; starch or lactose as excipients; stearates as lubricants; various sweetening or flavouring agents. For capsules the dosage unit may contain a liquid carrier like fatty oils. Likewise coatings of sugar or enteric agents may be part of the dosage unit. The pharmaceutical compositions may also be emulsions of the compound(s) and a lipid forming a micellular emulsion.

For parenteral, subcutaneous, intradermal or topical administration the pharmaceutical composition may include a sterile diluent, buffers, regulators of tonicity and antibacterials. The active compound may be prepared with carriers that protect against degradation or immediate elimination from the body, including implants or microcapsules with controlled release properties. For intravenous administration the preferred carriers are physiological saline or phosphate buffered saline.

Dosages

In one embodiment, the pharmaceutical composition is in unit dosage form. In such embodiments, each unit dosage form typically comprises 0.1-500 mg, such as 0.1-200 mg, e.g. 0.1-100 mg, of the compound.

More generally, the compound are preferably administered in an amount of about 0.1-250 mg per kg body weight per day, such as about 0.5-100 mg per kg body weight per day.

For compositions adapted for oral administration for systemic use, the dosage is normally 0.5 mg to 1 g per dose administered 1-4 times daily for 1 week to 12 months depending on the disease to be treated.

The dosage for oral administration of the composition in order to prevent diseases or conditions is normally 1 mg to 100 mg per kg body weight per day. The dosage may be administered once or twice daily for a period starting 1 week before the exposure to the disease until 4 weeks after the exposure.

For compositions adapted for rectal use for preventing diseases, a somewhat higher amount of the compound is usually preferred, i.e. from approximately 1 mg to 100 mg per kg body weight per day.

For parenteral administration, a dose of about 0.1 mg to about 100 mg per kg body weight per day is convenient. For intravenous administration, a dose of about 0.1 mg to about 20 mg per kg body weight per day administered for 1 day

to 3 months is convenient. For intraarticular administration, a dose of about 0.1 mg to about 50 mg per kg body weight per day is usually preferable. For parenteral administration in general, a solution in an aqueous medium of 0.5- 2% or more of the active ingredients may be employed.

For topical administration on the skin, a dose of about 1 mg to about 5 g administered 1-10 times daily for 1 week to 12 months is usually preferable.

Combination treatment

In an intriguing embodiment of the present invention, the compound of the general formula (I) or (Ia) or (Ib) is used therapeutically in combination with one or more other chemotherapeutic agents. Examples of such chemotherapeutic agents are those selected from daunorubicin, docetaxel, prednisone, dexamethasone, decadron, altretamine, amifostine, aminoglutethimide, dactinomycin, anastrozole, asparaginase, bicalutamide, bleomycin, busulfan, carboplatin, carmustine, chlorambucil, chlorodeoxyadenosine, cisplatin, cytosine arabinoside, dacarbazine, doxorubicin, epirubicin, estramustine, diethylstilbestrol, fludarabine, flutamide, 5-fluorouracil, gemcitabine, goserelin, idarubicin, irinotecan, levamisole, lomustine, mechlorathamine, alkeran, mercaptopurine, taxol (e.g. paclitaxel). In particular, the further chemotherapeutic agent is selected from taxanes such as Taxol, Paclitaxel and Docetaxel.

Thus, with respect to the use and the method of treatment defined herein, the medicament may further comprise one or more other chemotherapeutic agents.

With respect to the pharmaceutical composition defined herein, such a composition may further comprise one or more other chemotherapeutic agents.

EXAMPLES

For nuclear magnetic resonance ¹ H NMR spectra (300 MHz) and ¹³ C NMR (75.6) chemical shift values (δ) (in ppm) are quoted, unless otherwise specified, for deuteriochloroform solutions relative to tetramethylsilane (δ= 0.0) or chloroform (δ = 7.25) or deuteriochloroform (δ = 76.81 for ¹³ C NMR) standards. The value of a multiplet, either defined (dublet (d), triplet (t) quartet (q)) or not (m) at he approximate mid point is given unless a range is quoted, (bs) indicates a broad singlet. The organic solvents used were anhydrous.

The following abbreviations have been used throughout:

BOC terf-butyloxycarbonyl

DCM dichloromethane

DIC diisoproylcarbodiimide

DMAP dimethylaminopryridine

DMF N,N-dimethylformamide EDC N-(dimethylaminopropyl)-N ^' -ethylcarbodiimide

EtOAc ethyl acetate

NMR nuclear magnetic resonance rt room temperature

TFA trifluoro acetic acid TLC thin layer chromatography

Starting materials can be prepared as described in the literature, e.g. in WO 2005/097107.

General Procedure 1: Formation of BOC-protected amino acid ester derivatives from phenols of general formula (H).

The BOC-protected amino acid (12.0 mmol) was dissolved in DCM (20 mL), cooled in an ice bath under argon and DIC (6.0 mmol) was added drop-wise with stirring. After 1 Vi h the mixture was filtered, concentrated, redissolved in pyridine (10 mL) and the phenol of general formula (II) (2.0 mmol) was added

with stirring. The mixture was stirred at room temperature overnight, concentrated three times with toluene. The residue was purified by chromatography (1% methanol in DCM) or crystallization to afford BOC- protected amino acid ester derivatives from phenols of general formula (II).

General Procedure 2: Formation of BOC-protected amino acid ester derivatives from phenols of general formula (III).

The phenol of general formula (III) (0.42 mmol) and the BOC-protected amino acid (0.59 mmol) were dissolved in DCM, EDC (0.71 mmol) and DMAP (10 mg) were added with stirring and the mixture was left at room temperature overnight, transferred to a separatory funnel with EtOAc/H ₂ O and shaken. The aqueous phase was extracted twice more with EtOAc. The combined organic layers were washed with brine, dried (MgSO ₄ ) and concentrated. The residue was purified by chromatography (1% methanol in DCM or mixtures of petroleum ether and EtOAc) to afford BOC-protected amino acid ester derivatives from phenols of general formula (III).

General Procedure 3: Removal of BOC-protecting group from BOC-protected amino acid ester derivatives of phenols of general formula (H) or general formula (HI) using HCI to yield compounds of general formula (IV) or (V).

The BOC-protected amino acid ester derivative of phenol of general formula (II) or (III)) was dissolved in DCM or methanol and HCI in diethyl ether (1 M) was added. When TLC showed no remaining starting material the mixture was concentrated and if necessary purified by crystallization to afford compounds of general formula (IV) or (V).

General Procedure 4: Removal of BOC-protecting group from BOC-protected amino acid ester derivatives of phenols of general formula (H) or general formula (HI) using TFA to yield compounds of general formula (IV) or (V).

The BOC-protected amino acid ester derivative of phenol of general formula (II) or (III)) was dissolved in TFA. After 30 minutes the mixture was concentrated

twice with toluene and if necessary purified by crystallization to afford compounds of general formula (IV) or (V).

General Procedure 5: Formation of N,N-dimethylamino acid esterderivatives from phenols of general formula (H) or (HI).

The phenol of general formula (II) or (III) (0.27 mmol) and the N, N- dimethylamino acid (0.78 mmol) were dissolved in DMF, EDC (0.88 mmol) and DMAP (0.27 mmol) were added with stirring. The mixture was stirred at room temperature overnight, concentrated, extracted with EtOAc/H ₂ O. The aqueous layer was back-extracted twice with EtOAc, and the combined organic layers were washed with brine, dried (MgSO ₄ ) and concentrated. The residue was purified by crystallization to afford compound of general formula (IV) or (V).

Preparation 1 : (2S)-4-(6.7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3- vhphenyl 2-(te rt-butoxycarbonylamino')propanoate (compound 1).

General procedure 2. Starting materials: 6,7-difluoro-3-(4-hydroxy-phenyl)-3- (4-methoxy-phenyl)-l,3-dihydro-indol-2-one and λ/-BOC-L-alanine. ¹ H-NMR (CDCI ₃ ) δ 8.95 (bs, IH), 7.27 (d, 2H), 7.13 (d, 2H), 7.03 (d, 2H), 6.95-6.75 (m, 4H), 5.17 (d, IH), 4.56 (q, IH), 3.80 (s, 3H), 1.53 (d, 3H), 1.45 (s, 9H).

Preparation 2 : (2S)-4-(6,7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3- vHphenyl 2-(tert-butoxycarbonylamino) 3-phenylpropanoate (compound 2).

General procedure 2. Starting materials: 6,7-difluoro-3-(4-hydroxy-phenyl)-3- (4-methoxy-phenyl)-l,3-dihydro-indol-2-one and /V-BOC-L-phenylalanine^H- NMR (CDCI ₃ ) δ 8.60 (bs, IH), 7.40-7.15 (m, 9H), 7.0-6.80 (m 6H), 5.09 (d, IH), 4.82 (q, IH), 3.80 (s, 3H), 3.23 (d, 2H), 1.46 (s, 9H).

Preparation 3: ( 2S)-4-(6.7-difluoro-2-oxo-3-p-tolylindolin-3-yl )phenyl 2-ftert- butoxycarbonylamino') 3-phenylpropanoate (compound 3).

General procedure 2. Starting materials: 6,7-difluoro-3-(4-hydroxy-phenyl)-3-p- tolyl-l,3-dihydro-indol-2-one and /V-BOC-L-phenylalanine. ¹ H-NMR (CDCI ₃ ) δ 7.78 (bs, IH), 7.30-7.35 (m, 15H) 4.97 (d, IH), 4.71 (q, IH), 3.14 (d, 2H), 2.25( s, 3H), 1.36 (s, 9H).

Preparation 4: (2S. 2'S ^{^} ) 4.4 ^/ -f6.7-difluoro-2-oxoindoline-3.3-diyl tois(4.1- phenylene) bis(2-(te rt-butoxycarbonylamino) 3-propanoate (compound 4).

General procedure 1. Starting materials: 6,7-difluoro-3,3-bis(4-hydroxyphenyl)- indol-2-one and λ/-BOC-L-alanine. ¹ H-NMR (CDCI3) δ 9.17 (bs, IH), 7.17 (d, 4H) 6.97 (d, 4H), 6.85-6.70 (m, 2H), 5.07 (d, 2H), 4.46 (q, 2H), 1.45 (s, 6H), 1.38 (s, 18H).

Preparation 5: (2S. 2'S ¹ ) 4.4'-(6.7-difluoro-2-oxoindoline-3.3-diyl 1bis(4.1- phenylene) bis(2-(te rt-butoxycarbonylamino ^' )acetate (compound 5).

General procedure 1. Starting materials: 6,7-difluoro-3,3-bis(4-hydroxyphenyl)- indol-2-one and λ/-BOC-glycine. ¹ H-NMR (CDCI3) δ 8.27 (bs, IH), 7.17 (d, 4H) 6.97 (d, 4H), 6.88-6.73 (m, 2H), 5.01 (bs, 2H), 4.10 (d, 4H), 1.39 (s, 18H).

Preparation 6: (2S, 2'S) 4.4'-(6.7-difluoro-2-oxoindoline-3.3-diynbis(4.1- phenylene) bis(2-(tert-butoxycarbonyl(methyl)amino) 3-methylbutanoate (compound 6).

General procedure 1. Starting materials: 6,7-difluoro-3,3-bis(4-hydroxyphenyl)- indol-2-one and λ/-BOC-N-methyl-L-valine. ¹ H-NMR (CDCI3) δ 8.15 (bs, IH),

7.18 (d, 4H) 6.96 (d, 4H), 6.87-6.69 (m, 2H), 4.37 (dd, 2H), 2.84 (d, 3H), 2.21 (m, 2H), 1.41 (s, 18H), 1.02 (d, 6H), 0.89 (d, 6H).

Example 1 : (2S)-4-(6.7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3-yl)p henyl 2-aminopropanoate hydrochloride (compound 1001).

General procedure 3. Starting materials: compound 1. ¹ H-NMR (DMSOd ₆ ) δ 11.59 (s, IH), 8.59 (bs, 3H), 7.4-6.8 (m, 1OH), 4.40 (bs. IH) 3.73 (s, 3H), 1.56 (bs, 3H).

Example 2: (25.2 ⁷ SW. 4'-(6.7-difluoro-2-oxoindoline-3.3-divnbis (4,1- phenylene) bis(2-aminopropanoate dihvdrochloride (compound 1002).

General procedure 3. Starting materials: compound 4. ¹ H-NMR (DMSOd ₆ ) δ 11.70 (s, IH), 8.69 (bs, 6H), 7.4-6.8 (m, 1OH), 4.39 (bs. 2H), 1.55 (bs, 6H).

Example 3: 4. 4'-(6.7-difluoro-2-oxoindoline-3,3-diyl)bis (4,1-phenylene) bis(2- aminoacetate) ditrifluoroacetic acid salt (compound 1003).

General procedure 4. Starting materials: compound 5. ¹ H-NMR (DMSOd ₆ ) δ 11.70 (s, IH), 8.43 (bs, 6H), 7.34-7.04 (m, 1OH), 4.13 (S, 4H).

Example 4: (2S.2'SV4. 4 ^> -(6.7-difluoro-2-oxoindoline-3.3-divnbis (4.1- phenylene) bis(3-methyl-2-(methylamino)butanoate) dihydrochloride (compound 1004).

General procedure 3. Starting materials: compound 6. ¹ H-NMR (DMSOd ₆ ) δ 11.71 (s, IH), 9.55 (bs, 4H), 7.4-7.0 (m, 1OH), 4.26 (bs. 2H), 2.66 (s, 6H), 2.48 (m, 2H), 1.17 (d, 6H); 1.05 (d, 6H).

Example 5: 4. 4 ^> -(6.7-difluoro-2-oxoindoline-3,3-diyl)bis (4.1-phenylene) bis(2- dimethylamino^acetatei (compound 1005V

General procedure 5. Starting materials: 6,7-difluoro-3,3-bis(4-hydroxyphenyl)- indol-2-one and λ/.λ/-dimethylglycine. ¹ H-NMR (DMSOd ₆ ) δ 11.66 (s, IH), 7.3- 7.0 (m, 1OH), 3.46 (s, 2H), 2.32 (s, 6H).

Example 6: (2S)-4-(6.7-difluoro-3-(4-methoxyphenyl)-2-oxoindolin-3-yl)p henyl 2-aminophenylpropanoate hydrochloride (compound 1006).

General procedure 3. Starting materials: compound 2. ¹ H-NMR (DMSOd ₆ ) δ 11.57 (s, IH), 8.71, 7.28-7.42 (m, 5H), 7.17 (d, 2H), 7.0-7.15 (m, 4H), 6.86- 6.98 (m, 4H), 4.58 (t, IH), 3.73 (s, 3H), 3.17 (d, 2H)

General procedure for the following examples

¹ H NMR spectra were recorded at ambient temperature with Mercury 200 (Varian) spectrometer. The HPLC measurements were performed on an Alliance 2695 Separations Module equipped with a Waters 2487 UV detector by LiChrospher RP Select B 4.0 x 250 mm column. Elemental analyses were obtained with a Carlo Erba EA 11 08 instrument. Melting points were measured

on a "Boetius" micro melting point apparatus and are uncorrected. Chromatographical purifications of compounds were performed by column chromatography (CC) on silicagel, 0.035-0.070 mm, (Acros) or by flash chromatography (FC) on a Biotage SPl system with a Flash 12+M silicagel column. All the solvents were purified before use by routine techniques.To isolate reaction products, the solvents were removed by evaporation using a vacuum rotary evaporator, the water bath temperature not exceeding 40 ⁰ C.

Various reagents were purchased from Acros Organics (Janssens Pharmaceuticalaan 3A, 2440 Geel, Belgium).

Example 7: 6.7-Difluoro-3-f4-fluoropheny0-l-(2-hvdroxyethyh-3-(4- hvdroxyphenvO-l,3-dihvdro-2H-indol-2-one (compound 1007)

compound 7 compound 1007

To a solution of 6,7-difluoro-3-(4-fluorophenyl)-3-(4-hydroxyphenyl)-l,3- dihydro-2/V-indol-2-one (compound 7) (0.20Og, 0.56 mmol) in dimethylformamide (0.5 ml_) successively 60% NaH in mineral oil (0.010 g, 0.25 mmol) and l,3-dioxalan-2-one (0.054 g, 0.62 mmol) were added. The reaction mixture was stirred at 140 ⁰ C for 18 h, cooled, and concentrated in vacuo. The residue was purified by CC (15 g of SiO ₂ ) with dichloromethane-ethanol (100:5) as eluent and twice by FC with dichlormethane-ethanol (gradient from 100:0 to 80:20) as eluent to give the title compound 1007 (0.070 g, 31%) as a foam, m.p. 80-83 ⁰ C. IH-NMR (DMSOd ₆ , HMDSO) δ: 3.65 (m, 2H); 3.93 (m, 2H); 4.94 (t, J=5.4 Hz, IH); 6.70 (d, .7=8.8 Hz, 2H); 6.95 (d, J=8.8 Hz, 2H); 7.03-7.22 (m, 6H); 9.50 (s, IH). Anal. Calcd for C ₂₂ H ₁₆ F ₃ NO ₃ ■ 0.5 H ₂ O, %: C 64.71, H 4.20, N 3.43. Found, %: C 64.76, H 4.29, N 3.58.

Example 8: 4-r6.7-Difluoro-3-(4-fluorophenyh-2-oxo-2.3-dihydro-lH-indol -3- yllphenyl acrylate (compound 1008). 4-f6,7-Difluoro-3-(4-fluorophenyl)-2-oxo- 2,3-dihydro-lH-indol-3-yl1phenyl 3-morpholinopropanoate (compound 1009) and 4-f6 _r 7-Difluoro-3-(4-fluorophenyl)-2-oxo-2 _f 3-dihydro-lH-indol-3-yl1phenyl 3-(4-methylpiperazino ^' )propanoate (compound 1010)

O NCH ₃

To a solution of 6,7-difluoro-3-(4-fluorophenyl)-3-(4-hydroxyphenyl)-l,3- dihydro-2H-indol-2-one (compound 7) (0.18Og, 0.5 mmol) and triethylamine (0.22 mL, 1.58 mmol) in dichloromethane (5 ml_) at -10 ⁰ C acryloyl chloride (0.042 mL, 0.52 mmol) was added and the resulting mixture was stirred at this temperature for 3 h. The reaction mixture was supplemented with dichloromethane (45 mL), washed with sat. NaCI solution (4 x 10 mL), and dried (Na ₂ SO ₄ ). The solvent was evaporated and the residue was purified by FC with a mixture of petroleum ether and ethyl acetate-dichloromethane, 1: 1 (gradient from 100:0 to 30:70) as eluent to give the title compound 1008 (0.058 g, 27%) as an oil which solidified on standing. ¹ H-NMR (CDCI ₃ , HMDSO) δ: 6.01 (dd, J=1.4, 10.2 Hz, IH); 6.30 (dd, J=IO.2, 17.2 Hz, IH); 6.59 (dd, J=1.4, 17.2 Hz, IH); 6.78-6.93 (m, 2H); 6.99 (t, .7=8.8 Hz, 2H); 7.09 (d, J=8.6 Hz, 2H); 7.21 (dd, J=5.3, 8.8 Hz, 2H); 7.24 (d, 7=8.6 Hz, 2H); 8.62 (s, IH).

To a solution of 4-[6,7-difluoro-3-(4-fluorophenyl)-2-oxo-2,3-dihydro-lH-indo l- 3-yl]phenyl acrylate (compound 1008) (0.052 g, 0.13 mmol) in dry acetonitrile (3 mL) morpholine (0.012 mL, 0.14 mmol) followed by bismuth(III)

trifluoromethanesulfonate (0.0016 g, 0.0024 mmol) were added and the reaction mixture was stirred overnight at room temperature. The precipitated solid was filtered, crystallized from acetonitrile, and dried to give the title compound 1009 (0.032 g, 50%) as white crystals, m.p. 174-175°C. IH-NMR (DMSOd ₆ , HMDSO) δ: 2.33-2.46 (m, 4H, overlapped with DMSO); 2.57-2.87 (m, 4H, overlapped with DMSO); 3.50-3.62 (m, 4H); 6.97-7.26 (m, 10H); ~11- 12 (b s, IH). Anal. Calcd for C ₂₇ H ₂₃ F ₃ N ₂ O ₄ ■ 0.5 H ₂ O, %: C 64.15, H 4.79, N 5.54. Found, %: C 64.04, H 4.43, N 5.69.

Compound 1010 was prepared from 4-[6,7-difluoro-3-(4-fluorophenyl)-2-oxo- 2,3-dihydro-lH-indol-3-yl]phenyl acrylate (compound 1008) and 1- methylpiperazine in the same manner as for compound 1009 above in 61% yield was prepared. M.p. 149-151°C. IH-NMR (DMSO-d ₆ , HMDSO) δ: 2.13 (s, 3H); 2.30 (m, 4H); 2.40 (m, 4H); 2.68 (m, 4H); 6.97-7.27 (m, 10H); 11.61 (b s, IH). Anal. Calcd for C ₂₈ H ₂₆ F ₃ N ₃ O ₃ , %: C 66.00, H 5.14, N 8.25. Found, %: C 65.82, H 4.96, N 8.42.

Example 9: [3-(4-{[(Isopropoxycarbonyl)oxy1methoxy>phenyl)-3-(4- methoxyphenyl)-2-oxo-2,3-dihydro-lH-indol-l-yl ^" |methyl isopropyl carbonate (compound 1011)

compound 8

Ύ T o

K ₂ CO ₃ , Bu ₄ NHSO ₄ H ₂ O / CH ₂ CI ₂

To a suspension of 3-(4-hydroxyphenyl)-3-(4-methoxyphenyl)-l,3-dihydro-2H- indol-2-one (compound 8) (0.16 g, 0.48 mmol) in water (2.5 ml_) K ₂ CO ₃ (0.21 g, 1.5 mmol) was added and the mixture was stirred at r.t. for 5 min. Then Bu ₄ NHSO ₄ (0.17 g, 0.5 mmol), dichloromethane (2.5 ml_), and iodomethyl isopropyl carbonate (0.16 g, 0.65 mmol) were added and the resulting mixture was vigorously stirred at r.t. for 24 h. (The preparation of iodomethyl isopropyl carbonate was described in literature (Thomas, J. D.; Sloan, K. B. Tetrah. Lett. 2007, 48 (1), 109-112).) The aqueous layer was separated, the organic layer was supplemented with dichloromethane (10 ml_), washed with sat. NaCI solution, and dried (Na ₂ SO ₄ ). The solvent was evaporated and the residue was purified by CC (20 g of SiO ₂ ) with dichloromethane-ethanol (100: 1) as eluent to give the title compound compound 1011 (0.030 g, 11%) and compound 10 (0.11 g, 51%). Compound 1011: m.p. 47-49 ⁰ C. IH-NMR (DMSO-d ₆ , HMDSO) δ: 1.20 (d, 7=6.2 Hz, 6H); 1.21 (d, .7=6.2 Hz, 6H); 3.72 (s, 3H); 4.79 (septet,

7=6.2 Hz, IH); 4.79 (septet, 7=6.2 Hz, IH); 5.76 (s, 2H); 5.83 (s, 2H); 6.89 (d, .7=8.8 Hz, 2H); 7.02 (d, 7=9.0 Hz, 2H); 7.05 (d, J=8.8 Hz, 2H); 7.09 (d, J=9.0 Hz, 2H); 7.11-7.22 (m, IH); 7.25-7.45 (m, 3H). Anal. Calcd for C ₃₁ H ₃₃ NO ₉ , %: C 66.06, H 5.90, N 2.49. Found, %: C 66.16, H 5.87, N 2.51. Compound 9: m.p. 63-65°C. IH-NMR (DMSO-d _6/ HMDSO) δ: 1.21 (d, 7=6.2 Hz, 6H); 3.71 (s, 3H);

4.79 (septet, J=6.2 Hz, IH); 5.75 (s, 2H); 6.88 (d, J=8.9 Hz, 2H); 6.91-7.15 (m, 8H); 7.16-7.29 (m, 2H); 10.71 (s, IH). Anal. Calcd for C ₂₆ H ₂₅ NO ₆ , %: C 69.79, H 5.63, N 3.13. Found, %: C 69.68, H 5.60, N 3.13.

Example 10: Benzyl 2-r3-f4-r2-(benzyloxyV2-oxoethoxylphenylV3-(4- methoxyphenvπ-2-oxo-2.3-dihvdro-lH-indol-l-yllacetate (compound 1012^

Compound 10 and compound 1012 were prepared from 3-(4-hydroxyphenyl)-3- (4-methoxyphenyl)-l,3-dihydro-2AY-indol-2-one (compound 8) and benzyl 2- bromoacetate by the same protocol as for compound 1011 above in 15% and 78% yields, accordingly. Compound 10: m.p. 76-78°C. IH-NMR (DMSO-d ₆ , HMDSO) δ: 3.72 (s, 3H); 4.83 (s, 2H); 5.17 (s, 2H); 6.87 (d, 7=8.9 Hz, 2H); 6.87 (d, J=8.9 Hz, 2H); 6.90-7.02 (m, 2H); 7.04 (d, 7=8.9 Hz, 2H); 7.05 (d, .7=8.9 Hz, 2H); 7.16-7.28 (m, 2H); 7.33 (s, 5H); 10.69 (s, IH). Anal. Calcd for C ₃₀ H ₂₅ NO ₅ , %: C 75.14, H 5.25, N 2.92. Found, %: C 74.84, H 5.32, N 2.83.

Compound 1012: m.p. 57-58°C. IH-NMR (CDCI ₃ , HMDSO) δ: 3.75 (s, 3H); 4.56 (s, 2H); 4.60 (s, 2H); 5.17 (s, 2H); 5.21 (s, 2H); 6.69-6.80 (m, 5H); 7.03-7.39 (m, 17H). Anal. Calcd for C ₃₉ H ₃₃ NO ₇ , %: C 74.63, H 5.30, N 2.23. Found, %: C 74.48, H 5.30, N 2.19.

Example 11: 4-(3-{4-[(Anilinocarbonyl)oxy]phenyl>-6,7-difluoro-2-oxo- 2,3- dihvdro-lH-indol-3-vQphenyl N-phenylcarbamate (Compound 1013^

PhNCO

To a suspension of 6,7-difluoro-3,3-bis(4-hydroxyphenyl)-l,3-dihydro-2Ay-indol- 2-one (0.35 g, 1.0 mmol) in dry toluene under argon atmosphere phenyl isocyanate (0.24 ml_, 2.2 mmol) was added and the mixture was stirred at reflux for 3 h. The precipitated solid was filtered, purified by CC (30 g of SiO ₂ ) with dichloromethane-ethanol (100: 1) as eluent and crystallized from acetonitrile to give the title compound 1013 (0.40 g, 68%), m.p. 163-165°C. IH-NMR (DMSO- d _6/ HMDSO) δ: 6.94-7.38 (m, 2H); 7.04 (t, .7=7.2 Hz, 2H); 7.23 (s, 8H); 7.32 (t, .7=7.8 Hz, 4H); 7.49 (d, .7=8.0 Hz, 4H); 10.25 (br s, 2H); 11.67 (br s, IH). Anal. Calcd for C ₃₄ H ₂₃ F ₂ N ₃ O ₅ ■ 0.7 H ₂ O, %: C 67.59, H 4.07, N 6.95. Found, %: C 67.61, H 3.87, N 7.30.

Example 12: 4-r6.7-Difluoro-3-f4-fluorophenvh-2-oxo-2.3-dihydro-lH-indol -3- yllphenyl N.N-dimethylcarbamate (compound 1014)

compound 7 40%

To a solution of 6,7-difluoro-3-(4-fluorophenyl)-3-(4-hydroxyphenyl)-l,3- dihydro-2H-indol-2-one (compound 7) (0.110 g, 0.31 mmol), TEA (0.13 mL, 0.93 mmol), and DMAP (0.0076 g, 0.062 mmol) in THF (7 mL) was added dimethylcarbamyl chloride (0.033 mL, 0.34 mmol) and the reaction mixture was refluxed for 3 h. The solvent was evaporated, the residue was dissolved in CH ₂ CI ₂ (40 mL), washed with sat. NaCI solution (2 x 10 mL), and dried (Na ₂ SO ₄ ). The solvent was evaporated and the residue was purified by FC with CHCI ₃ - MeOH (gradient from 100:0 to 80:20) as eluent to give the title compound 1014 (0.053 g, 40%) as a white solid, m.p. 98-100 ⁰ C. ¹ H-NMR (DMSO-d ₆ , HMDSO) δ: 3.00 (s, 3H); 3.08 (s, 3H); 6.77-7.12 (m, 6H); 7.14-7.25 (m, 4H); 8.22 (s, IH). Anal. Calcd for C ₂₃ Hi ₇ F ₃ N ₂ O ₃ as if it contained 5.5% of ballast impurities, %: C 61.22, H 3.80, N 6.21. Found, %: C 61.25, H 3.80, N 6.07.

Example 13: 4-r6-Fluoro-3-f4-fluorophenyn-7-methyl-2-oxo-2.3-dihydro-lH- indol-3-yl]phenyl N.N-dimethylcarbamate (compound 1015) and 4-| ^" 6-Fluoro-3- (4-fluorophenyl)-7-methyl-2-oxo-2 _f 3-dihydro-lH-indol-3-yl1phenyl acrylate (compound 1016)

compound 1016 compound 11 60% compound1015

Compound 1015 was prepared from 6-fluoro-3-(4-fluorophenyl)-3-(4- hydroxyphenyl)-7-methyl-l,3-dihydro-2r/-indol-2-one (compound 11) in the same manner as for compound 1014 above in 60% yield. M. p. 263-265°C. ¹ H- NMR (DMSOd ₆ , HMDSO) δ: 2.19 (d, J=1.4 Hz, 3H); 2.89 (s, 3H); 3.01 (s, IH); 6.82 (dd, .7=8.4, 10.2 Hz, IH); 7.03-7.23 (m, 9H); 11.08 (s, IH).

To a solution of 6-fluoro-3-(4-fluorophenyl)-3-(4-hydroxyphenyl)-7-methyl-l,3 - dihydro-2H-indol-2-one (compound 11) (0.20Og, 0.6 mmol) and triethylamine (0.24 mL, 1.7 mmol) in dichloromethane (5 ml_) at ice bath temperature acryloyl chloride (0.046 mL, 0.60 mmol) was added and the resulting mixture was stirred at r.t. overnight. The reaction mixture was supplemented with dichloromethane (55 mL), washed with sat. NaCI solution (2 x 10 mL), and dried (Na ₂ SO ₄ ). The solvent was evaporated and the residue was purified by FC with a mixture of petroleum ether and ethyl acetate-dichloromethane, 1: 1 (gradient from 100:0 to 30:70) as eluent to give the title compound 1016 (0.15 g, 62%) as a white solid, m.p. 223-225°C. ¹ H-NMR (CDCI ₃ , HMDSO) δ: 2.20 (d, J=I.3 Hz, 3H); 6.15 (dd, .7=2.2, 9.5 Hz, IH); 6.39 (dd, J=9.5, 17.2 Hz, IH); 6.52 (dd, J=2.2, 17.2 Hz, IH); 6.83 (dd, J=8.3, 10.2 Hz, IH); 7.08-7.28 (m, 9H); 11.11 (s, IH).