PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

Title:

PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS

Document Type and Number:

WIPO Patent Application WO/2019/067982

Kind Code:

A3

Abstract:

This disclosure relates to the field of high scale production of recombinant Adeno-Associated Viruses (AAVs). The inventors have conceived of specific nucleic acid constructs that allow for high scale production of recombinant AAV particles in insect cells. Importantly, these nucleic constructs do not require the production of a heterologous AAP. This disclosure thus relates to a nucleic acid for producing AAV capsids in insect cells, where the nucleic acid includes a first open reading frame encoding the VP1, VP2, and VP3 proteins, and a second open reading frame encoding the Assembly-Activating Protein (AAP).

Inventors:

AYUSO EDUARD (FR)
ROBIN CECILE (FR)
PENAUD-BUDLOO MAGALIE (FR)
FRANCOIS ACHILLE (FR)
BLOUIN VÉRONIQUE (FR)
VANDENBERGHE LUK H (US)
MAURER ANNA CLAIRE (US)

Application Number:

PCT/US2018/053546

Publication Date:

May 09, 2019

Filing Date:

September 28, 2018

Export Citation:

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Assignee:

MASSACHUSETTS EYE & EAR INFIRMARY (US)
SCHEPENS EYE RES INST (US)
UNIV NANTES (FR)
CT HOSPITALIER UNIVERSITAIRE NANTES (FR)
INST NAT SANTE RECH MED (FR)

International Classes:

C07K14/005; C12N7/00

Domestic Patent References:

WO2016094783A1

2016-06-16

Foreign References:

US20170226160A1

2017-08-10

Other References: