PRODUCTION OF GUAR ALPHA-GALACTOSIDASE BY HOSTS TRANSFORMED WITH RECOMBINANT DNA METHODS

WIPO Patent Application WO/1987/007641

A1

The gene encoding an alpha-galactosidase from guar seed has been isolated and identified. It has been expressed in yeasts, e.g. Saccharomyces, Kluyveromyces and Hansenula, in bacteria, e.g. Bacillus, and in plants, e.g. Nicotiana. The resulting alpha-galactosidase is able to decrease the galactose content of galactomannans such as guar gum by splitting off 1-6 linked alpha-D-galactopyranosyl units attached to a main chain of 1-4 linked beta-mannopyranosyl units. Thus a commercially feasible process becomes available for producing alpha-galactosidases suitable for providing galactomannans having improved properties, which can meet the increasing demand for good quality polysaccharides. It has further been demonstrated that other alphagalactosidases, which show a positive immunological cross-reaction with an antibody raised against the alpha-galactosidase from guar, are also effective in decreasing the galactose content of galactomannans such as guar.

OVERBEEKE NICOLAAS (NL)
FELLINGER ARTHUR JOHANNES (NL)
HUGHES STEPHEN GLYN (GB)

PCT/EP1987/000296

December 17, 1987

June 02, 1987

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UNILEVER PLC (GB)
UNILEVER NV (NL)

A61K8/00; A61K8/02; A61K8/73; C12N1/14; C12N1/16; C12N1/19; A23K1/165; C12N1/20; C12N5/00; C12N5/10; C12N9/26; C12N9/28; C12N9/40; C12N15/00; C12N15/09; C12N15/56; C12N15/75; C12N15/81; C12N15/82; C12P19/14; (IPC1-7): C12N15/00; C12N9/40; C12P19/14; C12N1/18; C12N1/20; C12N5/00

EP0121960A2	1984-10-17
EP0066857A2	1982-12-15
EP0129268A2	1984-12-27
EP0120516B1	1991-10-23
EP0174585A2	1986-03-19
EP0127304A1	1984-12-05

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