Title:
PRODUCTION METHOD FOR INTESTINAL EPITHELIAL CELLS AND USE THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/261540
Kind Code:
A1
Abstract:
The problems addressed by the present invention are to provide a method for producing, via induction of differentiation in pluripotent stem cells, intestinal epithelial cells having a high cell count per area, and high kinetics prediction accuracy with respect to CYP3A4 substrates such as midazolam, and to provide the intestinal epithelial cells, a cell sheet, an evaluation method for a substance to be tested, a screening kit for a substance to be tested, and a cell preparation. According to the present invention, provided is a production method for intestinal epithelial cells, including a first differentiation step for differentiating pluripotent stem cells into intestinal stem cells, a proliferation step for causing the proliferation of the intestinal stem cells obtained during the differentiation step, and a second differentiation step for differentiating the intestinal stem cells obtained during the proliferation step into intestinal epithelial cells. The proliferation step is a step for putting the intestinal stem cells into a specific state.
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Inventors:
IMAKURA YUKI (JP)
MIMA SHINJI (JP)
OGURA IZUMI (JP)
YAMAZAKI NAO (JP)
MIMA SHINJI (JP)
OGURA IZUMI (JP)
YAMAZAKI NAO (JP)
Application Number:
PCT/JP2021/023893
Publication Date:
December 30, 2021
Filing Date:
June 24, 2021
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/071; A61K35/38; A61L27/38; A61P1/00; C12Q1/02; G01N33/15; G01N33/50; C12N5/10
Domestic Patent References:
| WO2018151307A1 | 2018-08-23 | |||
| WO2019156200A1 | 2019-08-15 | |||
| WO2014132933A1 | 2014-09-04 | |||
| WO2018151307A1 | 2018-08-23 | |||
| WO2019156200A1 | 2019-08-15 | |||
| WO2014165663A1 | 2014-10-09 |
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RIDEOUT III ET AL., NATURE GENETICS, vol. 24, 2000, pages 109
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NAKAJIMA ET AL., STEM CELLS, vol. 25, 2007, pages 983 - 985
KIM ET AL., CELL STEM CELL, vol. 1, 2007, pages 346 - 352
REVAZOVA ET AL., CLONING STEM CELLS, vol. 9, 2007, pages 432 - 449
REVAZOVA ET AL., CLONING STEM CELLS, vol. 10, 2008, pages 11 - 24
STRELCHENKO N. ET AL., REPROD BIOMED ONLINE, vol. 9, 2004, pages 623 - 629
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CHUNG Y. ET AL., CELL STEM CELL, vol. 2, 2008, pages 113 - 117
ZHANG X. ET AL., STEM CELLS, vol. 24, 2006, pages 2669 - 2676
WASSARMAN, P. M. ET AL., METHODS IN ENZYMOLOGY, vol. 365, 2003
MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 9523, 1998, pages 13726 - 13731
TURNPENNY ET AL., STEM CELLS, vol. 21, no. 5, 2003, pages 598 - 609
TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM J B ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM J B ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM C HMOON J I ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL I PMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
CAS , no. 1135306-29-4
A-L. UNGEL ET AL., EUR. J. PHRM. SCI., vol. 48, 2013, pages 166
TAKENAKA T. ET AL., DRUG METAB DISPOS, vol. 42, no. 1947, 2014, pages 54
PHARM RES, vol. 29, no. 3, March 2012 (2012-03-01), pages 651
TAKAYAMA K. ET AL., CELL. MOL. GASTROENTEROL. HEPATOL., vol. 8, 2019, pages 513 - 526
See also references of EP 4174170A4
CIBELLI ET AL., SCIENCE, vol. 280, 1998, pages 1256 - 1147
WILMUT ET AL., NATURE, vol. 385, 1997, pages 810
IRIYA ET AL., PROTEIN NUCLEIC ACID ENZYME, vol. 44, 1999, pages 892
BAGUISI ET AL., NATURE BIOTECHNOLOGY, vol. 17, 1999, pages 456
WAKAYAMA ET AL., NATURE, vol. 394, 1998, pages 369
NATURE GENETICS, vol. 22, 1999, pages 127
PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 14984
RIDEOUT III ET AL., NATURE GENETICS, vol. 24, 2000, pages 109
TACHIBANA ET AL.: "Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer", CELL, 2013
YU J ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAKAJIMA ET AL., STEM CELLS, vol. 25, 2007, pages 983 - 985
KIM ET AL., CELL STEM CELL, vol. 1, 2007, pages 346 - 352
REVAZOVA ET AL., CLONING STEM CELLS, vol. 9, 2007, pages 432 - 449
REVAZOVA ET AL., CLONING STEM CELLS, vol. 10, 2008, pages 11 - 24
STRELCHENKO N. ET AL., REPROD BIOMED ONLINE, vol. 9, 2004, pages 623 - 629
KLIMANSKAYAI. ET AL., NATURE, vol. 444, 2006, pages 481 - 485
CHUNG Y. ET AL., CELL STEM CELL, vol. 2, 2008, pages 113 - 117
ZHANG X. ET AL., STEM CELLS, vol. 24, 2006, pages 2669 - 2676
WASSARMAN, P. M. ET AL., METHODS IN ENZYMOLOGY, vol. 365, 2003
MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 9523, 1998, pages 13726 - 13731
TURNPENNY ET AL., STEM CELLS, vol. 21, no. 5, 2003, pages 598 - 609
TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM J B ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM J B ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM C HMOON J I ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL I PMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
CAS , no. 1135306-29-4
A-L. UNGEL ET AL., EUR. J. PHRM. SCI., vol. 48, 2013, pages 166
TAKENAKA T. ET AL., DRUG METAB DISPOS, vol. 42, no. 1947, 2014, pages 54
PHARM RES, vol. 29, no. 3, March 2012 (2012-03-01), pages 651
TAKAYAMA K. ET AL., CELL. MOL. GASTROENTEROL. HEPATOL., vol. 8, 2019, pages 513 - 526
See also references of EP 4174170A4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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