Title:
PRODUCTION METHOD FOR INTESTINAL TRACT CELLS DERIVED FROM PLURIPOTENT STEM CELLS AND HAVING CRYPT-VILLUS-LIKE STRUCTURES, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/168908
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a production method for intestinal tract cells derived from pluripotent stem cells and having crypt-villus-like structures, said method making it possible to achieve crypt-villus-like structures like those in a living intestinal tract as well as further achieve efficient differentiation into intestinal tract epithelial cells and functional improvement of said cells. According to the present invention, provided is a method for producing intestinal tract cells from pluripotent stem cells, said method including (1) a step for differentiating pluripotent stem cells into endoderm-like cells, (2) a step for differentiating the endoderm-like cells into intestinal tract stem cell-like cells, (3) a step for culturing the intestinal tract stem cell-like cells in the presence of certain factors, (4) a step for culturing the aforementioned cells and forming spheroids, (5) a step for differentiating the spheroids and forming intestinal tract organoids, and (6) a step for gas-phase/liquid-phase culturing of cells constituting the intestinal tract organoids in the presence of certain factors.
Inventors:
MATSUNAGA TAMIHIDE (JP)
IWAO TAKAHIRO (JP)
OGAWA ISAMU (JP)
QIU SHIMENG (JP)
IWAO TAKAHIRO (JP)
OGAWA ISAMU (JP)
QIU SHIMENG (JP)
Application Number:
PCT/JP2022/004220
Publication Date:
August 11, 2022
Filing Date:
February 03, 2022
Export Citation:
Assignee:
UNIV NAGOYA CITY PUBLIC UNIV CORP (JP)
International Classes:
C12N5/10; C12N5/0735; C12Q1/02; G01N33/50
Domestic Patent References:
| WO2020091020A1 | 2020-05-07 |
Foreign References:
| US20100047853A1 | 2010-02-25 |
Other References:
FREDRIK EO HOLMBERG, JAKOB B SEIDELIN, XIAOLEI YIN, BENJAMIN E MEAD, ZHIXIANG TONG, YUAN LI, JEFFREY M KARP, OLE H NIELSEN: "Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease", EMBO MOLECULAR MEDICINE (ONLINE), WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 9, no. 5, 1 May 2017 (2017-05-01), DE , pages 558 - 570, XP055560284, ISSN: 1757-4684, DOI: 10.15252/emmm.201607260
Attorney, Agent or Firm:
SIKs & Co. (JP)
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