Title:
PRODUCTION METHOD FOR POLYHYDROXYALKANOATE
Document Type and Number:
WIPO Patent Application WO/2024/101063
Kind Code:
A1
Abstract:
Provided is a PHA polymerization enzyme that has high substrate specificity and makes it possible to synthesize a PHA that has a comparatively high molecular weight. A production method for a polyhydroxyalkanoate according to the present invention includes a step for introducing a gene for a class I polyhydroxyalkanoate polymerization enzyme (excluding polyhydroxyalkanoate polymerization enzymes derived from Aeromonas caviae) into a microorganism and culturing the microorganism into which the gene was introduced in the presence of a carbon source. A mutant polyhydroxyalkanoate polymerization enzyme according to the present invention is derived from Plesiomonas shigelloides and is coded for by the base sequence of SEQ ID NO:4.
Inventors:
TSUGE TAKEHARU (JP)
RAMAMOORTHI SIVASHANKARI M (JP)
MIYAHARA YUKI (JP)
YAMAMOTO TOMOYOSHI (JP)
OZAWA SUGURU (JP)
RAMAMOORTHI SIVASHANKARI M (JP)
MIYAHARA YUKI (JP)
YAMAMOTO TOMOYOSHI (JP)
OZAWA SUGURU (JP)
Application Number:
PCT/JP2023/037027
Publication Date:
May 16, 2024
Filing Date:
October 12, 2023
Export Citation:
Assignee:
TOKYO INST TECH (JP)
TEIJIN LTD (JP)
TEIJIN LTD (JP)
International Classes:
C12N15/54; C12N9/10; C12P7/625
Domestic Patent References:
| WO2009147918A1 | 2009-12-10 |
Foreign References:
| JP2007125004A | 2007-05-24 |
Other References:
NUMATA KEIJI, MORISAKI KUMIKO; TOMIZAWA SATOSHI; OHTANI MISATO; DEMURA TAKU; MIYAZAKI MASAYUKI; NOGI YUICHI; DEGUCHI SHIGERU; DOI : "Synthesis of poly- and oligo(hydroxyalkanoate)s by deep-sea bacteria, Colwellia spp., Moritella spp., and Shewanella spp", POLYMER JOURNAL, NATURE PUBLISHING GROUP UK, LONDON, vol. 45, no. 10, 1 October 2013 (2013-10-01), London , pages 1094 - 1100, XP093170293, ISSN: 0032-3896, DOI: 10.1038/pj.2013.25
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Vibrio metschnikovii]", XP093170297, Database accession no. WP_154168902
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Ferrimonas marina]", XP093170298, Database accession no. WP_067661665
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Plesiomonas shigelloides]", XP093170300, Database accession no. WP_116546999
SIVASHANKARI RAMAMOORTHI M, MIERZATI MAIERWUFU; MIYAHARA YUKI; MIZUNO SHOJI; NOMURA CHRISTOPHER T.; TAGUCHI SEIICHI; ABE HIDEKI; T: "Exploring Class I polyhydroxyalkanoate synthases with broad substrate specificity for polymerization of structurally diverse monomer units", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 11, CH , XP093170303, ISSN: 2296-4185, DOI: 10.3389/fbioe.2023.1114946
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Vibrio metschnikovii]", XP093170297, Database accession no. WP_154168902
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Ferrimonas marina]", XP093170298, Database accession no. WP_067661665
DATABASE PROTEIN 3 June 2024 (2024-06-03), ANONYMOUS: "class I poly(R)-hydroxyalkanoic acid synthase [Plesiomonas shigelloides]", XP093170300, Database accession no. WP_116546999
SIVASHANKARI RAMAMOORTHI M, MIERZATI MAIERWUFU; MIYAHARA YUKI; MIZUNO SHOJI; NOMURA CHRISTOPHER T.; TAGUCHI SEIICHI; ABE HIDEKI; T: "Exploring Class I polyhydroxyalkanoate synthases with broad substrate specificity for polymerization of structurally diverse monomer units", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 11, CH , XP093170303, ISSN: 2296-4185, DOI: 10.3389/fbioe.2023.1114946
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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