Title:
PRODUCTION METHOD FOR PRODUCING PLURIPOTENT STEM CELL POPULATION
Document Type and Number:
WIPO Patent Application WO/2022/124298
Kind Code:
A1
Abstract:
In the present invention, cell death is suppressed when transferring pluripotent stem cells from adhesive culture to suspension culture. Pluripotent stem cells are adhesive cultured in liquid medium containing a PKCβ inhibitor and a TNKS inhibitor, and the pluripotent stem cells are then suspension cultured.
Inventors:
KAMBAYASHI SHO (JP)
HAYASHI YOHEI (JP)
TAKASAKI MAMI (JP)
HAYASHI YOHEI (JP)
TAKASAKI MAMI (JP)
Application Number:
PCT/JP2021/044881
Publication Date:
June 16, 2022
Filing Date:
December 07, 2021
Export Citation:
Assignee:
KANEKA CORP (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N5/10; C12N15/09
Domestic Patent References:
| WO2020203532A1 | 2020-10-08 | |||
| WO2016121737A1 | 2016-08-04 | |||
| WO2018015954A1 | 2018-01-25 | |||
| WO2019151386A1 | 2019-08-08 | |||
| WO2016016894A1 | 2016-02-04 | |||
| WO2013183777A1 | 2013-12-12 | |||
| WO2020039732A1 | 2020-02-27 | |||
| WO2018047941A1 | 2018-03-15 | |||
| WO2019151386A1 | 2019-08-08 |
Foreign References:
| JP2012175962A | 2012-09-13 |
Other References:
OLMER R, TISSUE ENGINEERING: PART C, vol. 18, no. 10, 2012, pages 772 - 784
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
YU J, SCIENCE, vol. 318, 2007, pages 1917 - 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
YU J, SCIENCE, vol. 318, 2007, pages 1917 - 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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