Title:
PRODUCTION OF PANCREATIC PROCARBOXY-PEPTIDASE B, ISOFORMS AND MUTEINS THEREOF, AND THEIR USE
Document Type and Number:
WIPO Patent Application WO2000061727
Kind Code:
A3
Abstract:
The invention relates to a method for producing pancreatic carboxy-peptidase B or an isoform or a mutein of carboxy-peptidase B, whereby: (a) a natural or unnatural precursor form of carboxy-peptidase B is expressed in a secretory manner in a microorganism; (b) the precursor form expressed in a secretory manner is purified, and; (c) the purified precursor form is converted into the active carboxy-peptidase B or into the isoform or the mutein thereof by means of an enzymatic treatment. The invention also relates to a nucleic acid construct, to its use in the aforementioned method, and to the production thereof, as well as to a host cell, the production thereof and its use in the method for producing pancreatic procarboxy-peptidase B or an isoform or a mutein thereof.
Inventors:
HABERMANN PAUL
Application Number:
PCT/EP2000/002571
Publication Date:
February 01, 2001
Filing Date:
March 23, 2000
Export Citation:
Assignee:
AVENTIS PHARMA GMBH (DE)
International Classes:
C12N1/19; C12N9/48; C12N9/64; C12N15/57; C12N15/09; C12P21/02; C12R1/84; (IPC1-7): C12N15/57; C12N9/48; C12N1/19; C07K14/62
Domestic Patent References:
| WO1995014096A1 | 1995-05-26 | |||
| WO1996002637A1 | 1996-02-01 | |||
| WO1996023064A1 | 1996-08-01 |
Other References:
HOCULI E ET AL: "GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, November 1988 (1988-11-01), pages 1321 - 1325, XP002916185, ISSN: 0733-222X
REVERTER DAVID ET AL: "Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 6, 6 February 1998 (1998-02-06), pages 3535 - 3541, XP002148710, ISSN: 0021-9258
REVERTER DAVID ET AL: "Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 6, 6 February 1998 (1998-02-06), pages 3535 - 3541, XP002148710, ISSN: 0021-9258
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