Title:
PRODUCTION OF TRANSGENIC AVIANS USING SPERM-MEDIATED TRANSFECTION
Document Type and Number:
WIPO Patent Application WO2003024199
Kind Code:
A3
Abstract:
The present invention relates to novel methods of producing transgenic avians, preferably chickens, wherein the incorporated transgene may be expressed as a constituent protein of the white of a hard-shell egg. The present invention provides sperm-mediated transfer for the introduction to an avian egg of a transgene encoding a heterologous polypeptide. The avian sperm may be irradiated before the transgenic gene is incorporated therein. Transgenic genes may be incorporated into avian sperm by lipofection, electroporation, restriction enzyme mediated integration (REMI) or similar methods. The modified avian sperm may then be delivered to an avian oocyte by microinjection, intracytoplasmic sperm injection (ICSI) or artificial insemination, or by natural coitus after the modified avian sperm are returned to a male bird. Heterologous nucleic acid may be integrated directly into the genomic nucleic acid of the oocyte or after first integrating the heterologous nucleic acid into the nucleic acid of a male germ cell and subsequent delivery of the transgenic male germ cell to an oocyte. Alternatively, the heterologous nucleic acid may be a episome within the sperm, or within the derivative zygote formed by the fusion of the sperm and the recipient oocyte, and may replicate independently of the zygote genome. Co-segregation of the episome with the replicated ooctye genome into all of the daughter cells may be induced by the heterologous nucleic acid having a centromeric body derived from, for example, a chromosome of a chicken.
More Like This:
Inventors:
RAPP JEFFREY C (US)
SUTRAVE PRAMOD (US)
SUTRAVE PRAMOD (US)
Application Number:
PCT/US2002/030156
Publication Date:
July 01, 2004
Filing Date:
September 23, 2002
Export Citation:
Assignee:
AVIGENICS INC (US)
RAPP JEFFREY C (US)
SUTRAVE PRAMOD (US)
RAPP JEFFREY C (US)
SUTRAVE PRAMOD (US)
International Classes:
C12N15/85; (IPC1-7): C12N15/00
Domestic Patent References:
| WO2000075300A2 | 2000-12-14 | |||
| WO1999006534A1 | 1999-02-11 | |||
| WO1997047739A1 | 1997-12-18 | |||
| WO2002063293A1 | 2002-08-15 | |||
| WO2003014344A2 | 2003-02-20 | |||
| WO2002079447A2 | 2002-10-10 | |||
| WO1999042569A1 | 1999-08-26 |
Foreign References:
| JP2001309736A | 2001-11-06 |
Other References:
IVARIE ET AL.: "Avian transgenesis: progress towards the promise", TRENDS IN BIOTECH., vol. 21, no. 1, January 2003 (2003-01-01), pages 14 - 19, XP004397632
SUB. ET AL.: "Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into reciepient embryos", MOL. REPROD., vol. 65, no. 4, August 2003 (2003-08-01), pages 389 - 395, XP002967884
HARVEY ET AL.: "Validating the hen as a bioreactor for the production of exogenous proteins in egg white", POULTRY SCI., vol. 82, no. 6, June 2003 (2003-06-01), pages 927 - 930, XP002967885
LAMPARD ET AL.: "Secretion of foreign proteins mediated by chicken lysozyme gene regulatory sequences", BIOCHEM. CELL BIOL., vol. 80, no. 6, 2002, pages 777 - 788, XP002975510
JEONG ET AL.: "Migration activity of chicken gonadal primordial germ cells (gPGCs) and post-transfer localization of LacZ-transfected gPGCs in the embryonic gonads", ASIAN-AUSTRALIAN J. ANIMAL SCI., vol. 15, no. 9, September 2002 (2002-09-01), pages 1227 - 1231, XP002967886
HAN ET AL.: "Production of germline chimeras by transfer of chicken gonadal primordial germ cells maintained in vitro for an extended period", THERIOGENOLOGY, vol. 58, no. 8, November 2002 (2002-11-01), pages 1531 - 1539, XP001179706
SPEKSNIJDER ET AL.: "Germline chimeric chickens from FACS-sorted donor cells", MOL. REPROD. DEVELOP., vol. 52, January 1999 (1999-01-01), pages 33 - 42, XP000974895
SALTER ET AL.: "Enhancement of spontaneous bursal lymphoma frequency by serotype 2 Marek's disease vaccine", AVIAN PATHOLOGY, vol. 28, no. 2, April 1999 (1999-04-01), pages 147 - 154, XP000943871
WEI ET AL.: "Selection of genetically modified chicken blastodermal cells by magnetic-activated cell sorting", POULTRY SCI., vol. 80, no. 12, December 2001 (2001-12-01), pages 1671 - 1678, XP002975511
ISHIDA ET AL.: "Production of human monoclonal and polyclonal antibodies in TransChromo animals", CLONING AND STEM CELLS, vol. 4, no. 1, 2002, pages 91 - 102, XP002970309
MOHAMMED ET AL.: "Deposition of genetically engineered human antibodies into the egg yolk of hens", IMMUNOTECHNOL., vol. 4, 1998, pages 115 - 125, XP000992747
SAYEGH ET AL.: "Avian B cell development: lessons from transgenic models", VETERINARY IMMUNOL. AND IMMUNOPATHOL., vol. 72, 1999, pages 31 - 37, XP002975512
VICK ET AL.: "Transgenic birds from transformed primordial germ cells", PROC. R. SOC. LOND., vol. 251, 1993, pages 179 - 182, XP000997980
THORAVAL ET AL.: "Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors", TRANSGENIC RES., vol. 4, 1995, pages 369 - 377, XP002095225
LOVE ET AL.: "Transgenic birds by DNA microinjection", BIO/TECHNOLOGY, vol. 12, January 1994 (1994-01-01), pages 60 - 63, XP002918749
HARVEY ET AL.: "Expression of exogenous protein in the egg white of transgenic chickens", NATURE BIOTECH., vol. 19, April 2002 (2002-04-01), pages 396 - 399, XP002973268
FURUTA ET AL.: "Expression of exogenous genes at the germinal ridge of germline chimeric chickens", ANIMAL CELL TECHNOL., vol. 12, 2002, pages 275 - 279, XP002975513
SQUIRES ET AL.: "Liposome mediated DNA transfer to chicken sperm cells", ANIMAL BIOTECH., vol. 4, no. 1, 1993, pages 71 - 88, XP002975514
SHUMAN: "Production of transgenic birds", EXPERIENTIA, vol. 47, no. 9, 15 September 1991 (1991-09-15), pages 897 - 905, XP000274373
WU ET AL.: "Effects of electroporation on quality of rooster semen", ASIAN-AUSTRALASIAN J., vol. 8, no. 2, 1995, pages 175 - 178
MURRAY ET AL.: "Transgenic Animals in Agriculture", 1999, article SQUIRES ET AL.: "Status of sperm-mediated delivery methods of gene transfer", pages: 87 - 95, XP002975515
PETITTE: "The avian germline and strategies for the production of transgenic chickens", J. POULTRY SCI., vol. 39, no. 4, October 2002 (2002-10-01), pages 205 - 228, XP000271528
SUB. ET AL.: "Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into reciepient embryos", MOL. REPROD., vol. 65, no. 4, August 2003 (2003-08-01), pages 389 - 395, XP002967884
HARVEY ET AL.: "Validating the hen as a bioreactor for the production of exogenous proteins in egg white", POULTRY SCI., vol. 82, no. 6, June 2003 (2003-06-01), pages 927 - 930, XP002967885
LAMPARD ET AL.: "Secretion of foreign proteins mediated by chicken lysozyme gene regulatory sequences", BIOCHEM. CELL BIOL., vol. 80, no. 6, 2002, pages 777 - 788, XP002975510
JEONG ET AL.: "Migration activity of chicken gonadal primordial germ cells (gPGCs) and post-transfer localization of LacZ-transfected gPGCs in the embryonic gonads", ASIAN-AUSTRALIAN J. ANIMAL SCI., vol. 15, no. 9, September 2002 (2002-09-01), pages 1227 - 1231, XP002967886
HAN ET AL.: "Production of germline chimeras by transfer of chicken gonadal primordial germ cells maintained in vitro for an extended period", THERIOGENOLOGY, vol. 58, no. 8, November 2002 (2002-11-01), pages 1531 - 1539, XP001179706
SPEKSNIJDER ET AL.: "Germline chimeric chickens from FACS-sorted donor cells", MOL. REPROD. DEVELOP., vol. 52, January 1999 (1999-01-01), pages 33 - 42, XP000974895
SALTER ET AL.: "Enhancement of spontaneous bursal lymphoma frequency by serotype 2 Marek's disease vaccine", AVIAN PATHOLOGY, vol. 28, no. 2, April 1999 (1999-04-01), pages 147 - 154, XP000943871
WEI ET AL.: "Selection of genetically modified chicken blastodermal cells by magnetic-activated cell sorting", POULTRY SCI., vol. 80, no. 12, December 2001 (2001-12-01), pages 1671 - 1678, XP002975511
ISHIDA ET AL.: "Production of human monoclonal and polyclonal antibodies in TransChromo animals", CLONING AND STEM CELLS, vol. 4, no. 1, 2002, pages 91 - 102, XP002970309
MOHAMMED ET AL.: "Deposition of genetically engineered human antibodies into the egg yolk of hens", IMMUNOTECHNOL., vol. 4, 1998, pages 115 - 125, XP000992747
SAYEGH ET AL.: "Avian B cell development: lessons from transgenic models", VETERINARY IMMUNOL. AND IMMUNOPATHOL., vol. 72, 1999, pages 31 - 37, XP002975512
VICK ET AL.: "Transgenic birds from transformed primordial germ cells", PROC. R. SOC. LOND., vol. 251, 1993, pages 179 - 182, XP000997980
THORAVAL ET AL.: "Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors", TRANSGENIC RES., vol. 4, 1995, pages 369 - 377, XP002095225
LOVE ET AL.: "Transgenic birds by DNA microinjection", BIO/TECHNOLOGY, vol. 12, January 1994 (1994-01-01), pages 60 - 63, XP002918749
HARVEY ET AL.: "Expression of exogenous protein in the egg white of transgenic chickens", NATURE BIOTECH., vol. 19, April 2002 (2002-04-01), pages 396 - 399, XP002973268
FURUTA ET AL.: "Expression of exogenous genes at the germinal ridge of germline chimeric chickens", ANIMAL CELL TECHNOL., vol. 12, 2002, pages 275 - 279, XP002975513
SQUIRES ET AL.: "Liposome mediated DNA transfer to chicken sperm cells", ANIMAL BIOTECH., vol. 4, no. 1, 1993, pages 71 - 88, XP002975514
SHUMAN: "Production of transgenic birds", EXPERIENTIA, vol. 47, no. 9, 15 September 1991 (1991-09-15), pages 897 - 905, XP000274373
WU ET AL.: "Effects of electroporation on quality of rooster semen", ASIAN-AUSTRALASIAN J., vol. 8, no. 2, 1995, pages 175 - 178
MURRAY ET AL.: "Transgenic Animals in Agriculture", 1999, article SQUIRES ET AL.: "Status of sperm-mediated delivery methods of gene transfer", pages: 87 - 95, XP002975515
PETITTE: "The avian germline and strategies for the production of transgenic chickens", J. POULTRY SCI., vol. 39, no. 4, October 2002 (2002-10-01), pages 205 - 228, XP000271528
Download PDF:
Previous Patent: DEVICE AND METHOD FOR AUTOMATIC IRRIGATION
Next Patent: PRODUCTION OF TRANSGENIC BIRDS USING STAGE X PRIMORDIAL GERM CELLS
Next Patent: PRODUCTION OF TRANSGENIC BIRDS USING STAGE X PRIMORDIAL GERM CELLS