Title:
PROMOTER PCALM2 AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/166413
Kind Code:
A1
Abstract:
A promoter pCALM2 and an application thereof. The nucleotide sequence of the promoter pCALM2 is as shown in SEQ ID NO: 1. The promoter pCALM2 can be applied to recombinant adeno-associated virus, can enable an exogenous gene to be efficiently expressed in the prosencephalon, and has a prospect of wide application in the fields of neural loop tracing and gene therapy.
Inventors:
WANG JINGYI (CN)
CHEN YEFEI (CN)
ZHU YUYUAN (CN)
LIN JIANBANG (CN)
ZHANG JIANQING (CN)
XU DONGDONG (CN)
LI MENGQI (CN)
BAI YANYANG (CN)
LU ZHONGHUA (CN)
CHEN YEFEI (CN)
ZHU YUYUAN (CN)
LIN JIANBANG (CN)
ZHANG JIANQING (CN)
XU DONGDONG (CN)
LI MENGQI (CN)
BAI YANYANG (CN)
LU ZHONGHUA (CN)
Application Number:
PCT/CN2021/138035
Publication Date:
August 11, 2022
Filing Date:
December 14, 2021
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12N15/113; C12N15/65; C12N15/864
Foreign References:
| CN112725342A | 2021-04-30 | |||
| CN112680443A | 2021-04-20 |
Other References:
DATABASE GenBank NCBI; ANONYMOUS : "Mus musculus CaMII gene for calmodulin, exon 1 and flanking region", XP055957368
MATSUO, K. ; IKESHIMA, H. ; SHIMODA, K. ; UMEZAWA, A. ; HATA, J.I. ; MAEJIMA, K. ; NOJIMA, H. ; TAKANO, T.: "Expression of the rat calmodulin gene II in the central nervous system: a 294-base promoter and 68-base leader segment mediates neuron-specific gene expression in transgenic mice", MOLECULAR BRAIN RESEARCH, ELSEVIER SCIENCE BV, AMSTERDAM, NL, vol. 20, no. 1-2, 1 October 1993 (1993-10-01), NL , pages 9 - 20, XP023999215, ISSN: 0169-328X, DOI: 10.1016/0169-328X(93)90106-Y
KORTVELY ELOD, GULYA KAROLY: "Calmodulin, and various ways to regulate its activity", LIFE SCIENCE, PERGAMON PRESS, OXFORD, GB, vol. 74, no. 9, 1 January 2004 (2004-01-01), GB , pages 1065 - 1070, XP055957372, ISSN: 0024-3205, DOI: 10.1016/j.lfs.2003.07.026
KARKERA J D, FRIEDBERG F: "Sequence of the human CALM II calmodulin gene promoter region", MOLECULAR BIOLOGY REPORTS, SPRINGER NETHERLANDS, NL, vol. 25, no. 3, 1 July 1998 (1998-07-01), NL , pages 193 - 195, XP055957379, ISSN: 0301-4851, DOI: 10.1023/A:1016561809397
BASTING TYLER, XU JIAXI, MUKERJEE SNIGDHA, EPLING JOEL, FUCHS ROBERT, SRIRAMULA SRINIVAS, LAZARTIGUES ERIC: "Glutamatergic neurons of the paraventricular nucleus are critical contributors to the development of neurogenic hypertension", THE JOURNAL OF PHYSIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 596, no. 24, 1 December 2018 (2018-12-01), GB , pages 6235 - 6248, XP055957386, ISSN: 0022-3751, DOI: 10.1113/JP276229
SATO KEIICHIRO, HAMASAKI YUMI, FUKUI KIYOSHIRO, ITO KAZUKI, MIYAMICHI KAZUNARI, MINAMI MASABUMI, AMANO TAIJU: "Amygdalohippocampal Area Neurons That Project to the Preoptic Area Mediate Infant-Directed Attack in Male Mice", THE JOURNAL OF NEUROSCIENCE, SOCIETY FOR NEUROSCIENCE, US, vol. 40, no. 20, 13 May 2020 (2020-05-13), US , pages 3981 - 3994, XP055957387, ISSN: 0270-6474, DOI: 10.1523/JNEUROSCI.0438-19.2020
ROOPASHRI HOLEHONNUR;JONATHAN A LUONG;DUSHYANT CHATURVEDI;ANTHONY HO;SRIHARI K LELLA;MATTHEW P HOSEK;JONATHAN E PLOSKI: "Adeno-associated viral serotypes produce differing titers and differentially transduce neurons within the rat basal and lateral amygdala", BMC NEUROSCIENCE, BIOMED CENTRAL, LONDON, GB, vol. 15, no. 1, 18 February 2014 (2014-02-18), GB , pages 28, XP021177935, ISSN: 1471-2202, DOI: 10.1186/1471-2202-15-28
PAPALE, A. ET AL.: "Viral vector approaches to modify gene expression in the brain.", JOURNAL OF NEUROSCIENCE METHODS, vol. 185, no. 1, 15 December 2009 (2009-12-15), XP026748078, DOI: 10.1016/j.jneumeth.2009.08.013
TOUTENHOOFD, S.L. ET AL.: "Characterization of the human CALM2 calmodulin gene and comparison of the transcriptional activity of CALM1, CALM2 and CALM3.", CELL CALCIUM., vol. 23, no. 5, 31 May 1998 (1998-05-31), XP002580659
MATSUO, K. ; IKESHIMA, H. ; SHIMODA, K. ; UMEZAWA, A. ; HATA, J.I. ; MAEJIMA, K. ; NOJIMA, H. ; TAKANO, T.: "Expression of the rat calmodulin gene II in the central nervous system: a 294-base promoter and 68-base leader segment mediates neuron-specific gene expression in transgenic mice", MOLECULAR BRAIN RESEARCH, ELSEVIER SCIENCE BV, AMSTERDAM, NL, vol. 20, no. 1-2, 1 October 1993 (1993-10-01), NL , pages 9 - 20, XP023999215, ISSN: 0169-328X, DOI: 10.1016/0169-328X(93)90106-Y
KORTVELY ELOD, GULYA KAROLY: "Calmodulin, and various ways to regulate its activity", LIFE SCIENCE, PERGAMON PRESS, OXFORD, GB, vol. 74, no. 9, 1 January 2004 (2004-01-01), GB , pages 1065 - 1070, XP055957372, ISSN: 0024-3205, DOI: 10.1016/j.lfs.2003.07.026
KARKERA J D, FRIEDBERG F: "Sequence of the human CALM II calmodulin gene promoter region", MOLECULAR BIOLOGY REPORTS, SPRINGER NETHERLANDS, NL, vol. 25, no. 3, 1 July 1998 (1998-07-01), NL , pages 193 - 195, XP055957379, ISSN: 0301-4851, DOI: 10.1023/A:1016561809397
BASTING TYLER, XU JIAXI, MUKERJEE SNIGDHA, EPLING JOEL, FUCHS ROBERT, SRIRAMULA SRINIVAS, LAZARTIGUES ERIC: "Glutamatergic neurons of the paraventricular nucleus are critical contributors to the development of neurogenic hypertension", THE JOURNAL OF PHYSIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 596, no. 24, 1 December 2018 (2018-12-01), GB , pages 6235 - 6248, XP055957386, ISSN: 0022-3751, DOI: 10.1113/JP276229
SATO KEIICHIRO, HAMASAKI YUMI, FUKUI KIYOSHIRO, ITO KAZUKI, MIYAMICHI KAZUNARI, MINAMI MASABUMI, AMANO TAIJU: "Amygdalohippocampal Area Neurons That Project to the Preoptic Area Mediate Infant-Directed Attack in Male Mice", THE JOURNAL OF NEUROSCIENCE, SOCIETY FOR NEUROSCIENCE, US, vol. 40, no. 20, 13 May 2020 (2020-05-13), US , pages 3981 - 3994, XP055957387, ISSN: 0270-6474, DOI: 10.1523/JNEUROSCI.0438-19.2020
ROOPASHRI HOLEHONNUR;JONATHAN A LUONG;DUSHYANT CHATURVEDI;ANTHONY HO;SRIHARI K LELLA;MATTHEW P HOSEK;JONATHAN E PLOSKI: "Adeno-associated viral serotypes produce differing titers and differentially transduce neurons within the rat basal and lateral amygdala", BMC NEUROSCIENCE, BIOMED CENTRAL, LONDON, GB, vol. 15, no. 1, 18 February 2014 (2014-02-18), GB , pages 28, XP021177935, ISSN: 1471-2202, DOI: 10.1186/1471-2202-15-28
PAPALE, A. ET AL.: "Viral vector approaches to modify gene expression in the brain.", JOURNAL OF NEUROSCIENCE METHODS, vol. 185, no. 1, 15 December 2009 (2009-12-15), XP026748078, DOI: 10.1016/j.jneumeth.2009.08.013
TOUTENHOOFD, S.L. ET AL.: "Characterization of the human CALM2 calmodulin gene and comparison of the transcriptional activity of CALM1, CALM2 and CALM3.", CELL CALCIUM., vol. 23, no. 5, 31 May 1998 (1998-05-31), XP002580659
Attorney, Agent or Firm:
BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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