Title:
PROTEIN FOR AFFINITY-SEPARATION MATRIX
Document Type and Number:
WIPO Patent Application WO/2012/133349
Kind Code:
A1
Abstract:
The present invention addresses the problem of developing a technique for producing a novel modified protein ligand that maximizes binding capacity and binding efficiency with respect to a target molecule when said ligand is immobilized via a lysine residual (side-chain epsilon-amino group) that allows an efficient carrier-immobilization reaction. Provided are: a modified protein containing a sequence comprising protein A, the typical protein ligand used for affinity purification, mutated by substitution of all of the lysine residuals therein with other amino acids and with lysine attached to the end thereof; and an affinity-separation matrix wherein said modified protein is immobilized on a water-insoluble carrier via reductive amination or the like. This affinity-separation matrix is characterized by high binding capacity with respect to a target molecule even with a low quantity of immobilized ligands.
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Inventors:
YOSHIDA SHINICHI (JP)
MURATA DAI (JP)
TAIRA SHUNICHI (JP)
MURATA DAI (JP)
TAIRA SHUNICHI (JP)
Application Number:
PCT/JP2012/057824
Publication Date:
October 04, 2012
Filing Date:
March 26, 2012
Export Citation:
Assignee:
KANEKA CORP (JP)
YOSHIDA SHINICHI (JP)
MURATA DAI (JP)
TAIRA SHUNICHI (JP)
YOSHIDA SHINICHI (JP)
MURATA DAI (JP)
TAIRA SHUNICHI (JP)
International Classes:
C12N15/09; C07K14/31; C07K17/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Domestic Patent References:
| WO2010110288A1 | 2010-09-30 | |||
| WO1997017361A1 | 1997-05-15 | |||
| WO2006004067A1 | 2006-01-12 | |||
| WO2010110288A1 | 2010-09-30 |
Foreign References:
| JP2008115151A | 2008-05-22 | |||
| JP2008266219A | 2008-11-06 | |||
| JP2007252368A | 2007-10-04 | |||
| JP2008115151A | 2008-05-22 | |||
| JP2008266219A | 2008-11-06 | |||
| JPH0231682A | 1990-02-01 | |||
| JPH04278091A | 1992-10-02 | |||
| JPH07170984A | 1995-07-11 | |||
| JPH06133782A | 1994-05-17 | |||
| JP2002238569A | 2002-08-27 | |||
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Other References:
HOBER S. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 40 - 47
LOW D. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 48 - 63
ROQUE A. C. A. ET AL., J. CHROMATOGR. A, vol. 1160, 2007, pages 44 - 55
KIHILBERG B. M. ET AL., J. BIOL. CHEM., vol. 267, 1992, pages 25583 - 25588
NAGATA ET AL.: "Real-time analysis of biomolecular interactions", 1998, SPRINGER-VERLAG TOKYO, pages: 41
BIOSCI. BIOTECH. BIOCHEM., vol. 61, 1997, pages 202 - 203
LOW D. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 48 - 63
ROQUE A. C. A. ET AL., J. CHROMATOGR. A, vol. 1160, 2007, pages 44 - 55
KIHILBERG B. M. ET AL., J. BIOL. CHEM., vol. 267, 1992, pages 25583 - 25588
NAGATA ET AL.: "Real-time analysis of biomolecular interactions", 1998, SPRINGER-VERLAG TOKYO, pages: 41
BIOSCI. BIOTECH. BIOCHEM., vol. 61, 1997, pages 202 - 203
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
Patent business corporation Yasutomi international patent firm (JP)
Patent business corporation Yasutomi international patent firm (JP)
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