Title:
PROTEIN COMPRISING REPEATEDLY ARRANGED POLY(AMINO ACID) OR POLYPEPTIDE, AND CHEMICAL SYNTHESIS METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/043631
Kind Code:
A1
Abstract:
The present invention establishes a process for producing a protein using a chemical synthesis method, in particular, using both an enzymatic polymerization method and a block copolymerization method. Provided are: a protein produced by the process and having properties that are similar or superior to those of natural silk protein; and products produced from the protein, such as a resin, a fiber, and a film. The present invention provides: a protein comprising a poly(amino acid) formed from a single amino acid and/or a polypeptide formed from a plurality of amino acids, the protein being a block copolymer which comprises at least two kinds of blocks that each are the poly(amino acid) or the polypeptide, the at least two kinds differing in the kind of amino acid and/or in amino-acid makeup, the blocks having been repeatedly arranged by block copolymerization; and products produced from the protein.
Inventors:
NUMATA KEIJI (JP)
TSUCHIYA KOUSUKE (JP)
TSUCHIYA KOUSUKE (JP)
Application Number:
PCT/JP2017/031317
Publication Date:
March 08, 2018
Filing Date:
August 31, 2017
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C07K14/00; C07K1/10; C08G69/10; C12P21/02; D01F4/02
Other References:
KOSUKE TSUCHIYA ET AL.: "Kozo Tanpakushitsu o Moho shita Multiblock Polypeptide no Gosei to Kozo Hyoka", DAI 26 KAI SYMPOSIUM ON POLYMERS AND BIOSCIENCES KOEN YOSHISHU, July 2016 (2016-07-01), pages 133 - 134
YAZAWA, K. ET AL.: "Papain-catalyzed synthesis of polyglutamate containing a nylon monomer unit.", POLYMERS, vol. 8, no. 5, 13 May 2016 (2016-05-13), pages 1 - 12, XP055471683, Retrieved from the Internet
TSUCHIYA, K. ET AL.: "Chemical synthesis of multiblock copolypeptides inspired by spider dragline silk proteins.", ACS MACRO LETTERS, vol. 6, January 2017 (2017-01-01), pages 103 - 106, XP055471685
YAZAWA, K. ET AL.: "Chemoenzymatic synthesis of a peptide containing nylon monomer units for thermally processable peptide material application.", POLYMER CHEMISTRY, vol. 8, 7 August 2017 (2017-08-07), pages 4172 - 4176, XP055471688
AGEITOS, J.M. ET AL.: "The benzyl ester group of amino acid monomers enhances substrate affinity and broadens the substrate specificity of the enzyme catalyst in chemoenzymatic copolymerization", BIOMACROMOLECULES, vol. 17, November 2015 (2015-11-01), pages 314 - 323, XP055471691, Retrieved from the Internet
KOSUKE TSUCHIYA ET AL.: "Kumo Ito o Moho shita Shinki Kozo Tanpaku Zairyo no Gosei", POLYMER PREPRINTS, vol. 64, no. 2, 2015, Japan
YAZAWA, K. ET AL.: "Papain-catalyzed synthesis of polyglutamate containing a nylon monomer unit.", POLYMERS, vol. 8, no. 5, 13 May 2016 (2016-05-13), pages 1 - 12, XP055471683, Retrieved from the Internet
TSUCHIYA, K. ET AL.: "Chemical synthesis of multiblock copolypeptides inspired by spider dragline silk proteins.", ACS MACRO LETTERS, vol. 6, January 2017 (2017-01-01), pages 103 - 106, XP055471685
YAZAWA, K. ET AL.: "Chemoenzymatic synthesis of a peptide containing nylon monomer units for thermally processable peptide material application.", POLYMER CHEMISTRY, vol. 8, 7 August 2017 (2017-08-07), pages 4172 - 4176, XP055471688
AGEITOS, J.M. ET AL.: "The benzyl ester group of amino acid monomers enhances substrate affinity and broadens the substrate specificity of the enzyme catalyst in chemoenzymatic copolymerization", BIOMACROMOLECULES, vol. 17, November 2015 (2015-11-01), pages 314 - 323, XP055471691, Retrieved from the Internet
KOSUKE TSUCHIYA ET AL.: "Kumo Ito o Moho shita Shinki Kozo Tanpaku Zairyo no Gosei", POLYMER PREPRINTS, vol. 64, no. 2, 2015, Japan
Attorney, Agent or Firm:
USHIKI Mamoru (JP)
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