PROTEIN CRYSTALLIZATION METHOD AND CRYSTALLIZATION DEVICE

Title:

PROTEIN CRYSTALLIZATION METHOD AND CRYSTALLIZATION DEVICE

Document Type and Number:

WIPO Patent Application WO/2018/159089

Kind Code:

A1

Abstract:

Provided are: an economically superior protein crystallization method that can efficiently identify conditions for crystallization using a small amount of a protein; and a crystallization device used in this method. According to the present invention, a part of a transparent sealed vessel 1 is formed of a molecular weight cut-off semipermeable membrane 2 that lets a precipitating agent pass through but blocks a protein. The transparent sealed vessel is filled with a solution of the protein, and then a precipitating agent solution, obtained by changing the concentration and/or pH of the precipitating agent, is supplied to the semipermeable membrane 2 in a continuous manner so that the protein is crystallized by the precipitating agent that permeates into the sealed vessel 1 through the semipermeable membrane 2.

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Inventors:

TANIGAWA NAOKI (JP)
TANAKA HIROAKI (JP)
TAKAHASHI SACHIKO (JP)
INAKA KOJI (JP)

Application Number:

PCT/JP2017/046880

Publication Date:

September 07, 2018

Filing Date:

December 27, 2017

Export Citation:

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Assignee:

CHIYODA CORP (JP)
CONFOCAL SCIENCE INC (JP)

International Classes:

C07K1/00; B01D9/02; B01D61/00; B01D61/14; C12M1/00

Domestic Patent References:

WO2002026342A1

2002-04-04

Foreign References:

JP2004045169A	2004-02-12
JP2004026528A	2004-01-29