Title:
PROTEIN EXPRESSION SYSTEM FOR SECRETING GLP-1 AND DERIVATIVE OF SAME, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/036859
Kind Code:
A1
Abstract:
Provided are a GLP-1 analogue mGLP-1 having an amino acid sequence as shown in SEQ ID NO: 4, and a recombinant expression vector that can secrete and express GLP-1 in food-grade lactic acid bacteria and a protein expression system secreting GLP-1. The vector is constructed by inserting a signal peptide spUSP45, an enhancer sequence LEISS, an enterokinase recognition site, the nucleic acid sequence of a humanized GLP-1 or the nucleic acid sequence of a humanized mGLP-1 into an expression vector pNZ8149, and the expression system is an intestinal tract normal bacterium transformed with the expression vector. Also provided is the use of the GLP-1 analogue, the nucleic acid sequence thereof, the expression vector and expression system in the preparation of a drug.
More Like This:
| WO/2000/055203 | ION EXCHANGE CHROMATOGRAPHIC SEPARATION OF GLP-1 AND RELATED PEPTIDES |
| JP3702181 | GLP-1 analog |
| WO/2004/029077 | METHOD FOR PRODUCING ACYLATED PEPTIDES |
Inventors:
JIN WANZHU (CN)
DONG MENG (CN)
DONG MENG (CN)
Application Number:
PCT/CN2017/098344
Publication Date:
February 28, 2019
Filing Date:
August 21, 2017
Export Citation:
Assignee:
INST ZOOLOGY CAS (CN)
International Classes:
C07K14/605; A61K38/26; A61P5/48; C12N1/21; C12N15/16; C12N15/74; C12R1/225; C12R1/23; C12R1/245; C12R1/25
Domestic Patent References:
| WO2011071957A1 | 2011-06-16 |
Foreign References:
| CN102796755A | 2012-11-28 | |||
| CN102329766A | 2012-01-25 | |||
| CN106011165A | 2016-10-12 | |||
| CN103804488A | 2014-05-21 | |||
| CN101535341A | 2009-09-16 |
Attorney, Agent or Firm:
PANAWELL & PARTNERS, LLC. (CN)
Download PDF: