PROTEIN HAVING EFFECT OF IMPROVING IMMUNOSTIMULATORY ACTIVITY OF LACTIC ACID BACTERIA EXOPOLYSACCHARIDE, AND FERMENTED MILK INCLUDING SAME AND METHOD FOR PRODUCING SAME

WIPO Patent Application WO/2022/080245

A1

The present invention is at least one protein selected from the group consisting of proteins (a) to (d) below. (a) A protein comprising the amino acid sequence represented by SEQ ID NO: 1; (b) a protein comprising the amino acid sequence represented by SEQ ID NO: 1, in which one or more amino acids other than serine, leucine, phenylalanine, serine, and aspartic acid at positions 334-338 are substituted, deleted, inserted and/or added, the protein also having the effect of improving immunostimulatory activity of lactic acid bacteria exopolysaccharide; (c) a protein comprising an amino acid sequence having at least 80% identity with the amino acid sequence represented by SEQ ID NO: 1, in which the amino acids corresponding to positions 334-338 of the amino acid sequence represented by SEQ ID NO: 1 are, from the N terminus, serine, leucine, phenylalanine, serine, and aspartic acid, the protein also having the effect of improving immunostimulatory activity of lactic acid bacteria exopolysaccharide; and (d) a protein comprising an amino acid sequence encoded by DNA hybridizing, under a strict condition, with the complementary strand of DNA comprising the nucleotide sequence represented by SEQ ID NO: 2, in which the amino acids corresponding to positions 334-338 of the amino acid sequence represented by SEQ ID NO: 1 are, from the N terminus, serine, leucine, phenylalanine, serine, and aspartic acid, the protein also having the effect of improving immunostimulatory activity of lactic acid bacteria exopolysaccharide.

FUJIWARA SHIN (JP)
MAKINO SEIYA (JP)

PCT/JP2021/037283

April 21, 2022

October 08, 2021

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MEIJI CO LTD (JP)

C12N15/31; A23C9/123; C07K14/335; C12N1/21; C12N15/63; C12P19/04; C12Q1/02; C12Q1/68

JP2005194259A	2005-07-21
JP2006520590A	2006-09-14

MAKINO, SEIYA: "Application of exopolysaccharides(EPS) produced from Lactobacillus delbrueckii ssp. bulgaricus, and studies on increasing the production of EPS", JOURNAL OF JAPAN SOCIETY FOR LACTIC ACID BACTERIA, vol. 24, no. 1, 1 January 2013 (2013-01-01), pages 10 - 17, XP055586897
PEI HAO, HUAJUN ZHENG, YAO YU, GUOHUI DING, WENYI GU, SHUTING CHEN, ZHONGHAO YU, SHUANGXI REN, MUNEHIRO ODA, TOMONOBU KONNO, SHENG: "Complete Sequencing and Pan-Genomic Analysis of Lactobacillus delbrueckii subsp. bulgaricus Reveal Its Genetic Basis for Industrial Yogurt Production", PLOS ONE, vol. 6, no. 1, 17 January 2011 (2011-01-17), pages 1 - 9, XP055287606, DOI: 10.1371/journal.pone.0015964
DATABASE Protein 2 April 2021 (2021-04-02), ANONYMOUS: "alpha(1,3)galactosyltransferase [Lactobacillus delbrueckii subsp. bulgaricus]", XP055922659, retrieved from Genbank Database accession no. BCT97414
FUJISAWA, MINORI ET AL.: "The effects of eps-gene deletion on the growth and EPS production in Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1", JAPANESE JOURNAL OF LACTIC ACID BACTERIA, vol. 31, no. 3, 1 January 2020 (2020-01-01), JP , pages 156, XP009535803, ISSN: 1343-327X

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