Title:
PROTEIN HAVING LACCASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2024/043159
Kind Code:
A1
Abstract:
Provided is a novel cupper efflux oxidase (CueO) variant having improved activity compared to wild types. A protein according to one or more embodiments of the present invention has laccase activity and comprises the amino acid sequence of [1] or [2]: [1] the amino acid sequence of SEQ ID NO: 14 into which mutations (a) and (b) have been introduced, mutation (a) being substitution of D360 with another amino acid other than D, and mutation (b) being at least one selected from substitution of G304 with another amino acid other than G, substitution of D373 with another amino acid other than D, and substitution of Q374 with another amino acid other than Q, the amino acid sequence including at least a region from position 29 to position 516; [2] the amino acid sequence having at least 90% sequence identity with the amino acid of [1], in which amino acid residues corresponding to position 360, position 304, position 373, and position 374 of the amino acid sequence of SEQ ID NO: 14 are identical to those of the amino acid sequences of [1].
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Inventors:
KONO TAKUNARI (JP)
UZURA ATSUKO (JP)
UMEDA MANA (JP)
UZURA ATSUKO (JP)
UMEDA MANA (JP)
Application Number:
PCT/JP2023/029678
Publication Date:
February 29, 2024
Filing Date:
August 17, 2023
Export Citation:
Assignee:
NAGASE & CO LTD (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12P7/00; H01M4/90; H01M8/1004; H01M8/1016; H01M8/16
Foreign References:
| JP2014003970A | 2014-01-16 | |||
| CN105176939A | 2015-12-23 |
Other References:
LINGLING ZHANG; HAIYANG CUI; GAURAO V. DHOKE; ZHI ZOU; DANIEL F. SAUER; MEHDI D. DAVARI; ULRICH SCHWANEBERG: "Engineering of Laccase CueO for Improved Electron Transfer in Bioelectrocatalysis by Semi‐Rational Design", CHEMISTRY - A EUROPEAN JOURNAL, JOHN WILEY & SONS, INC, DE, vol. 26, no. 22, 18 March 2020 (2020-03-18), DE, pages 4974 - 4979, XP071852091, ISSN: 0947-6539, DOI: 10.1002/chem.201905598
WANG HANQIAN, LIU XIAOQING, ZHAO JINTONG, YUE QINGXIA, YAN YUHUA, GAO ZENGQIANG, DONG YUHUI, ZHANG ZHIYONG, FAN YUNLIU, TIAN JIAN,: "Crystal structures of multicopper oxidase CueO G304K mutant: structural basis of the increased laccase activity", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 8, no. 1, 24 September 2018 (2018-09-24), US , pages 14252, XP093142365, ISSN: 2045-2322, DOI: 10.1038/s41598-018-32446-7
YUE, Q. et al. Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge. Bioresour. Bioprocess. 2017, vol. 4, no. 48, pp. 1-8
WANG HANQIAN, LIU XIAOQING, ZHAO JINTONG, YUE QINGXIA, YAN YUHUA, GAO ZENGQIANG, DONG YUHUI, ZHANG ZHIYONG, FAN YUNLIU, TIAN JIAN,: "Crystal structures of multicopper oxidase CueO G304K mutant: structural basis of the increased laccase activity", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 8, no. 1, 24 September 2018 (2018-09-24), US , pages 14252, XP093142365, ISSN: 2045-2322, DOI: 10.1038/s41598-018-32446-7
YUE, Q. et al. Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge. Bioresour. Bioprocess. 2017, vol. 4, no. 48, pp. 1-8
Attorney, Agent or Firm:
ENDO, MASAHARU (JP)
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