Title:
PROTEIN HYDROLYSATE, POLYPEPTIDE SOLUTION AND POLYPEPTIDE, PREPARATION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/085602
Kind Code:
A1
Abstract:
Provided are a protein hydrolysate, a polypeptide solution and a polypeptide, and a preparation method and use thereof. The method comprises steps of: adding extraction agent to brewer's spent grain and obtaining a crude protein or a crude protein solution; preparing a crude protein solution from the crude protein, adjusting pH of the crude protein solution to 6.5-8.5; adding an alkaline protease and heating to 45-65℃ to hydrolyze for 1-5 h, obtaining a protein hydrolysate; inactivating the protease in the protein hydrolysate, obtaining a polypeptide solution; separating the polypeptide solution by gel column, collecting peaks and pooling together, and obtaining an active polypeptide. The polypeptide has a hypoglycemic activity.
Inventors:
LI LIN (CN)
LI BING (CN)
TIAN YINGJUAN (CN)
CHEN LING (CN)
ZHU LIANG (CN)
LIU GUOQIN (CN)
SU JIANYU (CN)
LI XIAOXI (CN)
LI BING (CN)
TIAN YINGJUAN (CN)
CHEN LING (CN)
ZHU LIANG (CN)
LIU GUOQIN (CN)
SU JIANYU (CN)
LI XIAOXI (CN)
Application Number:
PCT/CN2010/077743
Publication Date:
July 21, 2011
Filing Date:
October 14, 2010
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
LI LIN (CN)
LI BING (CN)
TIAN YINGJUAN (CN)
CHEN LING (CN)
ZHU LIANG (CN)
LIU GUOQIN (CN)
SU JIANYU (CN)
LI XIAOXI (CN)
LI LIN (CN)
LI BING (CN)
TIAN YINGJUAN (CN)
CHEN LING (CN)
ZHU LIANG (CN)
LIU GUOQIN (CN)
SU JIANYU (CN)
LI XIAOXI (CN)
International Classes:
C12P21/06; A61K38/02; A61P3/10; C07K1/14; C07K2/00
Foreign References:
| CN101736065A | 2010-06-16 |
Other References:
LI, Z.D. ET AL.: "Studies on extraction of protein form brewer's spent grain by alcohol-alkali method", JOURNAL OF QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY (NATURAL SCIENCE EDITION), vol. 29, no. 1, February 2008 (2008-02-01), pages 19 - 21
LU, H. ET AL.: "Studies on extraction process of protein from brewer's spent grain by alcohol-alkali method", LIQUOR MAKING, vol. 28, no. 4, July 2001 (2001-07-01), pages 106 - 107
CAI J. ET AL.: "The hydrolysis of protein of brewer's spent grains with protmex", LIQUOR MAKING, vol. 28, no. 1, January 2001 (2001-01-01), pages 52 - 53
CAI J. ET AL.: "The hydrolysis of protein of brewer's spent grains with papain", CHINA WESTERN CEREALS & OILS TECHNOLOGY, June 2002 (2002-06-01), pages 38 - 40
LU, H. ET AL.: "Study on hydrolyzing protein in beer distiller's grains with neutral protease and papoid", BREWAGE OF CHINA, August 2001 (2001-08-01), pages 22 - 25
LU, H. ET AL.: "Studies on extraction process of protein from brewer's spent grain by alcohol-alkali method", LIQUOR MAKING, vol. 28, no. 4, July 2001 (2001-07-01), pages 106 - 107
CAI J. ET AL.: "The hydrolysis of protein of brewer's spent grains with protmex", LIQUOR MAKING, vol. 28, no. 1, January 2001 (2001-01-01), pages 52 - 53
CAI J. ET AL.: "The hydrolysis of protein of brewer's spent grains with papain", CHINA WESTERN CEREALS & OILS TECHNOLOGY, June 2002 (2002-06-01), pages 38 - 40
LU, H. ET AL.: "Study on hydrolyzing protein in beer distiller's grains with neutral protease and papoid", BREWAGE OF CHINA, August 2001 (2001-08-01), pages 22 - 25
Attorney, Agent or Firm:
GUANGZHOU HUAXUE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
广州市华学知识产权代理有限公司 (CN)
广州市华学知识产权代理有限公司 (CN)
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Claims: