Title:
PROTEOME ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2002/090970
Kind Code:
A1
Abstract:
A novel proteome analysis method whereby quantitative changes of proteins expressed in cells are monitored with the passage of time and thus the genomic expression state can be quantitatively analyzed. This method comprises adding a liquid culture medium containing an isotope-labeled amino aid to vital cells, culturing the cells and then collecting the same after a definite period of time, extracting proteins therefrom, fractionating the extracted proteins by developing by two-dimensional electrophoresis, and quantifying arbitrary proteins thus fractionated each based on the mass difference betweem a protein having the labeled amino acid and the protein free from the labeled amino acid.
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Inventors:
YOSHIZATO KATSUTOSHI (JP)
KRISTENSEN DAN BACH (DK)
KRISTENSEN DAN BACH (DK)
Application Number:
PCT/JP2002/004388
Publication Date:
November 14, 2002
Filing Date:
May 02, 2002
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
HIROSHIMA IND PROMOTION ORG (JP)
YOSHIZATO KATSUTOSHI (JP)
KRISTENSEN DAN BACH (DK)
HIROSHIMA IND PROMOTION ORG (JP)
YOSHIZATO KATSUTOSHI (JP)
KRISTENSEN DAN BACH (DK)
International Classes:
G01N27/62; C12Q1/02; C12Q1/37; G01N27/447; G01N33/483; G01N33/58; G01N33/68; (IPC1-7): G01N27/62; C12Q1/02; G01N27/447; H01J49/00
Domestic Patent References:
| WO1998019271A1 | 1998-05-07 | |||
| WO1999035502A1 | 1999-07-15 |
Other References:
JENSEN ET AL.: "Mass spectrometric detection for capillary isoelectric focusing separations of complex protein mixtures", ELECTROPHORESIS, vol. 21, no. 7, 2000, pages 1372 - 1380, XP002205644
JENSEN ET AL.: "Probing protromes using capillary isoelectric focusing-electrospray ionization fourier transform ion cyclotron resonance mass spectrometry", ANALYTICAL CHEMISTRY, vol. 71, no. 11, 1999, pages 2076 - 2084, XP002205643
ODA ET AL.: "Accurate quantitation of protein expression and site-specific phosphorylation", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 12, 1999, pages 6591 - 6596, XP001080471
PAPAGEORGOPOULUS ET AL.: "Measuring protein synthesis by mass isotopomer distribution analysis (MIDA)", ANALYTICAL BIOCHEMISTRY, vol. 267, no. 1, 1999, pages 1 - 16, XP002955812
JENSEN ET AL.: "Probing protromes using capillary isoelectric focusing-electrospray ionization fourier transform ion cyclotron resonance mass spectrometry", ANALYTICAL CHEMISTRY, vol. 71, no. 11, 1999, pages 2076 - 2084, XP002205643
ODA ET AL.: "Accurate quantitation of protein expression and site-specific phosphorylation", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 12, 1999, pages 6591 - 6596, XP001080471
PAPAGEORGOPOULUS ET AL.: "Measuring protein synthesis by mass isotopomer distribution analysis (MIDA)", ANALYTICAL BIOCHEMISTRY, vol. 267, no. 1, 1999, pages 1 - 16, XP002955812
Attorney, Agent or Firm:
Nishizawa, Toshio (37-10 Udagawa-ch, Shibuya-ku Tokyo, JP)
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