Title:
PURIFICATION METHOD FOR FLUORESCEIN-LABELED NUCLEOSIDE TRIPHOSPHATE
Document Type and Number:
WIPO Patent Application WO/2023/019670
Kind Code:
A1
Abstract:
A purification method for fluorescein-labeled nucleoside triphosphate, specifically comprising: purifying a fluorescein-labeled nucleoside triphosphate crude product by means of liquid chromatography, and collecting a target fraction, wherein a mobile phase comprises a first component and a second component, the first component is an aqueous n-hexylamine acetate solution, and the second component is selected from acetonitrile or methanol. According to the purification method, the nucleoside diphosphate in a fluorescein-labeled nucleoside triphosphate crude product which has a similar structure to nucleoside triphosphate and is difficult to separate can be separated from a target product, thereby obtaining fluorescein-labeled nucleoside triphosphate having a purity of greater than 99%. The purified fluorescein-labeled nucleoside triphosphate has good fluorescence intensity and high sensitivity, and meets the requirements of fluorescence in-situ hybridization experiments.
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Inventors:
HE LING (CN)
JIANG XIWEN (CN)
LAN SHILONG (CN)
JIANG XIWEN (CN)
LAN SHILONG (CN)
Application Number:
PCT/CN2021/118893
Publication Date:
February 23, 2023
Filing Date:
September 17, 2021
Export Citation:
Assignee:
DAAN GENE CO LTD (CN)
International Classes:
C07H19/10; C09K11/00
Foreign References:
| CN103484106A | 2014-01-01 | |||
| CN1940563A | 2007-04-04 | |||
| CN104910229A | 2015-09-16 | |||
| CN104693258A | 2015-06-10 |
Attorney, Agent or Firm:
SHANGHAI GOOIP CO., LTD. (CN)
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