Title:
PVA MEMBRANE IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/012041
Kind Code:
A1
Abstract:
Provided are a PVA film immobilized enzyme and a preparation method therefor. The PVA membrane immobilized enzyme comprises a PVA porous membrane and an enzyme embedded on the PVA porous membrane. The PVA porous membrane is a three-dimensional structured PVA porous membrane. The enzyme is any one selected from transaminase, D-lactate dehydrogenase, cyclohexanone monooxygenase, ketoreductase, alkene reductase, nitrilase, ammonia lyase, amino acid dehydrogenase, imine reductase, alcohol dehydrogenase, ammonium formate dehydrogenase, glucose 1-dehydrogenase and mutants thereof. The three-dimensional structured PVA porous membrane is used as a carrier to immobilize an enzyme in an embedding manner. The embedding and immobilization process is simple, conditions are mild, and the specific surface area is large. After embedding and immobilizing the enzyme in the PVA porous membrane, the enzyme is stable, and cannot be easily leached out in the process of use. The porous structure of the PVA porous membrane used can better transmit reactants and products, is suitable for use in continuous flow biochemical catalysis, and has wide applicability to enzymes.
Inventors:
HONG HAO (US)
JAMES (US)
XIAO YI (CN)
ZHANG NA (CN)
VYASA WILLIAMS (US)
CUI YUXIA (CN)
ZHAO JIADONG (CN)
GAO YANYAN (CN)
JAMES (US)
XIAO YI (CN)
ZHANG NA (CN)
VYASA WILLIAMS (US)
CUI YUXIA (CN)
ZHAO JIADONG (CN)
GAO YANYAN (CN)
Application Number:
PCT/CN2021/076756
Publication Date:
January 20, 2022
Filing Date:
February 18, 2021
Export Citation:
Assignee:
ASYMCHEM LIFE SCIENCE TIANJIN CO LTD (CN)
International Classes:
C12N11/084
Domestic Patent References:
| WO2011051145A1 | 2011-05-05 |
Foreign References:
| CN111560363A | 2020-08-21 | |||
| CN110964710A | 2020-04-07 | |||
| CN101979645A | 2011-02-23 | |||
| CN107893065A | 2018-04-10 | |||
| CN103668485A | 2014-03-26 | |||
| CN101054574A | 2007-10-17 |
Other References:
WANG CHENG, DONG SHUNFU;HAN LIQIN;FENG BO;ZHANG XIURONG;SUI CHUNHONG: "Immobilization of Glucoamylase Onto Electrospinning PVA Nanofiber Membrane via Chemical Process", HUAGONG XINXING CAILIAO - NEW CHEMICAL MATERIALS, ZHONGGUO HUAGONG XINXI ZHONGXIN, CN, vol. 41, no. 12, 31 December 2013 (2013-12-31), CN , XP055886739, ISSN: 1006-3536
ENZYME MICROB TECHNOL, vol. 31, pages 171 - 8
J PHARM SCI, vol. 89, pages 979 - 90
ANALYST, vol. 133, pages 697 - 701
CHEM SOC REV, vol. 40, pages 2567 - 92
ANAL CHEM, vol. 66, 1994, pages 1120A - 126
ENZYME MICROB TECHNOL, vol. 31, pages 171 - 8
J PHARM SCI, vol. 89, pages 979 - 90
ANALYST, vol. 133, pages 697 - 701
CHEM SOC REV, vol. 40, pages 2567 - 92
ANAL CHEM, vol. 66, 1994, pages 1120A - 126
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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