QPCR METHOD AND KIT HAVING INCREASED SPECIFICITY AND SENSITIVITY

Title:

QPCR METHOD AND KIT HAVING INCREASED SPECIFICITY AND SENSITIVITY

Document Type and Number:

WIPO Patent Application WO/2024/014734

Kind Code:

Abstract:

The present invention relates to a method for adding a com probe, which is a non-amplified non-fluorescent probe (combination or competition probe) through a method for controlling non-specific signals generated by the non-specific binding of fluorescent probes occurring in a high-concentration wild-type sample. Through a method for adding a com probe or a PCR kit capable of performing the method, the present invention inhibits non-specific signals generated by the binding of a fluorescent probe to a wild-type nucleic acid sequence, so as to enhance the specificity and positive detection of amplification signals of a mutant nucleic acid sequence, and thus can increase detection reliability.

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Inventors:

KIM JAE JONG (KR)
LIM SI-KYU (KR)
KYUNG AYOUNG (KR)

Application Number:

PCT/KR2023/008683

Publication Date:

January 18, 2024

Filing Date:

June 22, 2023

Export Citation:

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Assignee:

GENOTECH CORP (KR)

International Classes:

C12Q1/6848; C12Q1/6851

Foreign References:

KR20210073947A	2021-06-21
KR20150054633A	2015-05-20
CN113774168A	2021-12-10
CN113774169A	2021-12-10

Other References:

KIM JAE JONG, PARK HYOUNG MIN, KYOUNG A. YOUNG, LIM SI-KYU, CHA SUN HO, LEE J. EUGENE, PARK BYOUNG CHUL: "Introduction of STexS Ⅱ: implementing ‘Com probes’ to enhance specificity of high Tm mutant probes", RESEARCH SQUARE, 6 July 2022 (2022-07-06), XP093128646, Retrieved from the Internet [retrieved on 20240207], DOI: 10.21203/rs.3.rs-1717463/v1
KIM JAE JONG, PARK HYOUNG-MIN, KYOUNG A. YOUNG, LIM SI-KYU, CHA SUN HO, LEE J. EUGENE, PARK BYOUNG CHUL: "Com probe implemented STexS II greatly enhances specificity in SARS-CoV-2 variant detection", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 13, no. 1, 19 January 2023 (2023-01-19), US , pages 1036, XP093128649, ISSN: 2045-2322, DOI: 10.1038/s41598-022-24530-w

Attorney, Agent or Firm:

KANG, Seong-Hea (KR)

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