Title:
QUALITY ASSESSMENT OF INDUCED PLURIPOTENT CELLS BY REFERRING TO SPLICING SIGNATURES OF PLURIPOTENCY
Document Type and Number:
WIPO Patent Application WO/2013/024024
Kind Code:
A3
Abstract:
The present invention concerns a method of determining the pluripotency status of a cells comprising looking at the splicing signature in said cells, and correlating said splicing signature with the pluripotency status of the cells.
More Like This:
Inventors:
DEBONO-LAGNEAUX DELPHINE (FR)
DELEUZE JEAN-FRANCOIS (FR)
MULTON MARIE-CHRISTINE (FR)
ROCHER CORINNE (FR)
SPANAKIS EMMANUEL (FR)
THILL GILBERT (FR)
DELEUZE JEAN-FRANCOIS (FR)
MULTON MARIE-CHRISTINE (FR)
ROCHER CORINNE (FR)
SPANAKIS EMMANUEL (FR)
THILL GILBERT (FR)
Application Number:
PCT/EP2012/065681
Publication Date:
April 11, 2013
Filing Date:
August 10, 2012
Export Citation:
Assignee:
SANOFI SA (FR)
DEBONO-LAGNEAUX DELPHINE (FR)
DELEUZE JEAN-FRANCOIS (FR)
MULTON MARIE-CHRISTINE (FR)
ROCHER CORINNE (FR)
SPANAKIS EMMANUEL (FR)
THILL GILBERT (FR)
DEBONO-LAGNEAUX DELPHINE (FR)
DELEUZE JEAN-FRANCOIS (FR)
MULTON MARIE-CHRISTINE (FR)
ROCHER CORINNE (FR)
SPANAKIS EMMANUEL (FR)
THILL GILBERT (FR)
International Classes:
C12N5/074; C12N5/0735; C12Q1/68; G01N33/50
Domestic Patent References:
| WO2010118525A1 | 2010-10-21 |
Other References:
DATABASE Geneseq [online] 10 March 2005 (2005-03-10), "Human cartilage-related fibronectin EBD DNA fragment - SEQ ID 51.", XP002673403, retrieved from EBI accession no. GSN:ADV51120 Database accession no. ADV51120
SALOMONIS NATHAN ET AL: "Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 107, no. 23, June 2010 (2010-06-01), pages 10514 - 10519, XP002684311, ISSN: 0027-8424
M. PRITSKER: "Diversification of stem cell molecular repertoire by alternative splicing", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 102, no. 40, 1 January 2005 (2005-01-01), pages 14290 - 14295, XP055039458, ISSN: 0027-8424, DOI: 10.1073/pnas.0502132102
CLARA Y. CHEONG ET AL: "Alternative Splicing in Self-Renewal of Embryonic Stem Cells", STEM CELLS INTERNATIONAL, vol. 2011, 9 January 2011 (2011-01-09), pages 1 - 8, XP055039539, ISSN: 1687-966X, DOI: 10.4061/2011/560261
MARTINA HALLEGGER ET AL: "Alternative splicing: global insights", FEBS JOURNAL, vol. 277, no. 4, 1 February 2010 (2010-02-01), pages 856 - 866, XP055039466, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2009.07521.x
JULIAN P VENABLES ET AL: "Cancer-associated regulation of alternative splicing", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 16, no. 6, 1 June 2009 (2009-06-01), pages 670 - 676, XP055039474, ISSN: 1545-9993, DOI: 10.1038/nsmb.1608
A. R. GROSSO ET AL: "Tissue-specific splicing factor gene expression signatures", NUCLEIC ACIDS RESEARCH, vol. 36, no. 15, 1 January 2008 (2008-01-01), pages 4823 - 4832, XP055039471, ISSN: 0305-1048, DOI: 10.1093/nar/gkn463
GUSTAVO S. GUIMARÃES ET AL: "Identification of candidates for tumor-specific alternative splicing in the thyroid", GENES, CHROMOSOMES AND CANCER, vol. 45, no. 6, 1 June 2006 (2006-06-01), pages 540 - 553, XP055047915, ISSN: 1045-2257, DOI: 10.1002/gcc.20316
MUNGALL A J ET AL: "The DNA sequence and analysis of human chromosome 6", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 425, no. 6960, 23 October 2003 (2003-10-23), pages 805 - 811, XP008129728, ISSN: 0028-0836
RAI B S GILL ET AL: "Sulf2 gene is alternatively spliced in mammalian developing and tumour tissues with functional implications", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 414, no. 3, 24 November 2011 (2011-11-24), pages 468 - 473, XP028328919, ISSN: 0006-291X, [retrieved on 20110924], DOI: 10.1016/J.BBRC.2011.09.088
GOPALAKRISHNA-PILLAI SAILESH ET AL: "A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells", PLOS ONE, vol. 6, no. 6, E20663, June 2011 (2011-06-01), pages 1 - 10, XP002689366, ISSN: 1932-6203
SALOMONIS NATHAN ET AL: "Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 107, no. 23, June 2010 (2010-06-01), pages 10514 - 10519, XP002684311, ISSN: 0027-8424
M. PRITSKER: "Diversification of stem cell molecular repertoire by alternative splicing", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 102, no. 40, 1 January 2005 (2005-01-01), pages 14290 - 14295, XP055039458, ISSN: 0027-8424, DOI: 10.1073/pnas.0502132102
CLARA Y. CHEONG ET AL: "Alternative Splicing in Self-Renewal of Embryonic Stem Cells", STEM CELLS INTERNATIONAL, vol. 2011, 9 January 2011 (2011-01-09), pages 1 - 8, XP055039539, ISSN: 1687-966X, DOI: 10.4061/2011/560261
MARTINA HALLEGGER ET AL: "Alternative splicing: global insights", FEBS JOURNAL, vol. 277, no. 4, 1 February 2010 (2010-02-01), pages 856 - 866, XP055039466, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2009.07521.x
JULIAN P VENABLES ET AL: "Cancer-associated regulation of alternative splicing", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 16, no. 6, 1 June 2009 (2009-06-01), pages 670 - 676, XP055039474, ISSN: 1545-9993, DOI: 10.1038/nsmb.1608
A. R. GROSSO ET AL: "Tissue-specific splicing factor gene expression signatures", NUCLEIC ACIDS RESEARCH, vol. 36, no. 15, 1 January 2008 (2008-01-01), pages 4823 - 4832, XP055039471, ISSN: 0305-1048, DOI: 10.1093/nar/gkn463
GUSTAVO S. GUIMARÃES ET AL: "Identification of candidates for tumor-specific alternative splicing in the thyroid", GENES, CHROMOSOMES AND CANCER, vol. 45, no. 6, 1 June 2006 (2006-06-01), pages 540 - 553, XP055047915, ISSN: 1045-2257, DOI: 10.1002/gcc.20316
MUNGALL A J ET AL: "The DNA sequence and analysis of human chromosome 6", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 425, no. 6960, 23 October 2003 (2003-10-23), pages 805 - 811, XP008129728, ISSN: 0028-0836
RAI B S GILL ET AL: "Sulf2 gene is alternatively spliced in mammalian developing and tumour tissues with functional implications", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 414, no. 3, 24 November 2011 (2011-11-24), pages 468 - 473, XP028328919, ISSN: 0006-291X, [retrieved on 20110924], DOI: 10.1016/J.BBRC.2011.09.088
GOPALAKRISHNA-PILLAI SAILESH ET AL: "A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells", PLOS ONE, vol. 6, no. 6, E20663, June 2011 (2011-06-01), pages 1 - 10, XP002689366, ISSN: 1932-6203
Attorney, Agent or Firm:
RIBARD, Carin (Patent Department54 rue La Boétie, Paris, FR)
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