Title:
QUANTITATIVE SOFT AGAR COLONY FORMATION ASSAY USING TETRAZOLIUM THAT GENERATES WATER-SOLUBLE FORMAZAN
Document Type and Number:
WIPO Patent Application WO/2013/051590
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a quantitative soft agar colony formation assay which can be achieved rapidly and has high measurement accuracy. Specifically, the present invention relates to a method for evaluating the survival of a cell, comprising: a step of overlaying an agar containing the cell on an agar that is placed on the bottom surface of a container, further overlaying a culture medium on the agar containing the cell, and culturing the cell; a step of removing the culture medium, adding both tetrazolium that can generate water-soluble formazan and an electronic carrier, and culturing the cell; and a step of evaluating the survival of the cell employing the occurrence of the development of the color of the water-soluble formazan as an indicator.
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Inventors:
FURUKAWA TATSUHIKO (JP)
Application Number:
PCT/JP2012/075598
Publication Date:
April 11, 2013
Filing Date:
October 03, 2012
Export Citation:
Assignee:
UNIV KAGOSHIMA (JP)
FURUKAWA TATSUHIKO (JP)
FURUKAWA TATSUHIKO (JP)
International Classes:
C12Q1/04
Domestic Patent References:
| WO2005023786A1 | 2005-03-17 |
Other References:
"CytoselectTM 96well in vitro Shuyo Kanjusei Assay", COSMO BIO NEWS, no. 82, 2010, pages 3
TSUKATANI TADAYUKI. ET AL.: "Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts", J MICROBIOL METHODS, vol. 75, 2008, pages 109 - 116, XP023783195, DOI: doi:10.1016/j.mimet.2008.05.016
BUTTKE T M. ET AL.: "Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines", J IMMUNOL METHODS, vol. 157, 1993, pages 233 - 240, XP024351379, DOI: doi:10.1016/0022-1759(93)90092-L
CORY A H. ET AL.: "Use of an Aqueous Soluble Tetrazolium/Formazan Assay for Cell Growth Assays in Culture", CANCER COMMUN, vol. 3, 1991, pages 207 - 212, XP009009200
TSUKATANI TADAYUKI. ET AL.: "Distinction of Gram-positive and -negative bacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts with a selection medium", J GEN APPL MICROBIOL, vol. 57, December 2011 (2011-12-01), pages 331 - 339
TSUKATANI TADAYUKI. ET AL.: "Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts", J MICROBIOL METHODS, vol. 75, 2008, pages 109 - 116, XP023783195, DOI: doi:10.1016/j.mimet.2008.05.016
BUTTKE T M. ET AL.: "Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines", J IMMUNOL METHODS, vol. 157, 1993, pages 233 - 240, XP024351379, DOI: doi:10.1016/0022-1759(93)90092-L
CORY A H. ET AL.: "Use of an Aqueous Soluble Tetrazolium/Formazan Assay for Cell Growth Assays in Culture", CANCER COMMUN, vol. 3, 1991, pages 207 - 212, XP009009200
TSUKATANI TADAYUKI. ET AL.: "Distinction of Gram-positive and -negative bacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts with a selection medium", J GEN APPL MICROBIOL, vol. 57, December 2011 (2011-12-01), pages 331 - 339
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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