Title:
RALSTONIA EUTROPHA HAVING LOW ENDOTOXIN CONTENT, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/017318
Kind Code:
A1
Abstract:
The present application belongs to the technical field of microorganisms and provides Ralstonia eutropha having low endotoxin content and a use thereof. Further provided is a use of the reduction of enzymatic activity and/or expression of the H16_A0228 protein and/or the H16_B0917 protein of Ralstonia eutropha in the reduction of the endotoxin content of Ralstonia eutropha. The inactivation of the H16_A0228 and H16_B0917 proteins of Ralstonia eutropha can markedly reduce the endotoxin content of Ralstonia eutropha. The engineered Ralstonia eutropha having loss-of-function of the H16_A0228 protein and H16_B0917 protein has significantly lowered endotoxin content, while cell dry weight and PHA yield are significantly raised. The present application provides new gene and strain resources for the development of engineer PHA strains, provides an effective method for reducing the endotoxin content of PHAs, and has important significance for extending the application of PHAs in medical materials.
Inventors:
FENG JING (CN)
YANG XIAOYAN (CN)
YIN JIN (CN)
LI TENG (CN)
ZHANG HAOQIAN (CN)
YANG XIAOYAN (CN)
YIN JIN (CN)
LI TENG (CN)
ZHANG HAOQIAN (CN)
Application Number:
PCT/CN2023/108313
Publication Date:
January 25, 2024
Filing Date:
July 20, 2023
Export Citation:
Assignee:
SHENZHEN BLUEPHA BIOSCIENCES CO LTD (CN)
International Classes:
C12N15/74; C07K14/195; C12N1/21; C12N15/31; C12P7/625
Foreign References:
| CN115976088A | 2023-04-18 | |||
| US10968496B2 | 2021-04-06 | |||
| CN114480317A | 2022-05-13 | |||
| CN101008011A | 2007-08-01 |
Other References:
DATABASE Protein 17 April 2019 (2019-04-17), ANONYMOUS : "lipid A biosynthesis lauroyl acyltransferase [Cupriavidus necator H16] ", XP093131703, retrieved from NCBI Database accession no. QCC03602.1
DATABASE Protein 17 April 2019 (2019-04-17), ANONYMOUS : "lauroyl acyltransferase [Cupriavidus necator H16]", XP093131711, retrieved from NCBI Database accession no. QCB99338.1
WANG, ZIYU ET AL.: "Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties", METABOLIC ENGINEERING, vol. 67, 5 June 2021 (2021-06-05), XP086768060, DOI: 10.1016/j.ymben.2021.05.010
WANG ZIYU, ZHENG YIFEI, JI MENGKE, ZHANG XU, WANG HUAN, CHEN YUEMENG, WU QIONG, CHEN GUO‐QIANG: "Hyperproduction of PHA copolymers containing high fractions of 4‐hydroxybutyrate (4HB) by outer membrane‐defected Halomonas bluephagenesis grown in bioreactors", MICROBIAL BIOTECHNOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 15, no. 5, 1 May 2022 (2022-05-01), GB , pages 1586 - 1597, XP093131715, ISSN: 1751-7915, DOI: 10.1111/1751-7915.13999
DATABASE Protein 17 April 2019 (2019-04-17), ANONYMOUS : "lauroyl acyltransferase [Cupriavidus necator H16]", XP093131711, retrieved from NCBI Database accession no. QCB99338.1
WANG, ZIYU ET AL.: "Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties", METABOLIC ENGINEERING, vol. 67, 5 June 2021 (2021-06-05), XP086768060, DOI: 10.1016/j.ymben.2021.05.010
WANG ZIYU, ZHENG YIFEI, JI MENGKE, ZHANG XU, WANG HUAN, CHEN YUEMENG, WU QIONG, CHEN GUO‐QIANG: "Hyperproduction of PHA copolymers containing high fractions of 4‐hydroxybutyrate (4HB) by outer membrane‐defected Halomonas bluephagenesis grown in bioreactors", MICROBIAL BIOTECHNOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 15, no. 5, 1 May 2022 (2022-05-01), GB , pages 1586 - 1597, XP093131715, ISSN: 1751-7915, DOI: 10.1111/1751-7915.13999
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (CN)
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