Title:
RAPID TEST CARD FOR SIMULTANEOUSLY DETECTING PEDV AND TGEV, AND PREPARATION METHOD AND USE METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/218250
Kind Code:
A1
Abstract:
Disclosed are a rapid test card for simultaneously detecting porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), and a preparation method and a use method therefor. The rapid test card comprises a card shell (1) and a test strip (2) arranged in the card shell. The test strip (2) comprises a PVC bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad which are sequentially connected and pasted to the PVC bottom plate. A sample adding hole (3) and a result observation window (4) are formed in the card shell (1). An anti-PEDV specific monoclonal antibody and an anti-TGEV specific monoclonal antibody are fixed on the nitrocellulose membrane to form a first detection line (T1) and a second detection line (T2), respectively; and the anti-PEDV specific monoclonal antibody marked by colloidal gold and the anti-TGEV specific monoclonal antibody marked by colloidal gold are fixed on the combination pad. With only one instance of sample adding, the rapid test card can be used to simultaneously detect PEDV and TGEV in a pig excrement sample, and reactions are performed under unified conditions; and the rapid test card has advantages such as rapidness, high accuracy, high sensitivity, and simple operation.
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Inventors:
LI BIN (CN)
FAN BAOCHAO (CN)
GUO RONGLI (CN)
ZHOU JINZHU (CN)
ZHAO YONGXIANG (CN)
HE KONGWANG (CN)
NI YANXIU (CN)
CHANG XINJIAN (CN)
SHI DANYI (CN)
FAN BAOCHAO (CN)
GUO RONGLI (CN)
ZHOU JINZHU (CN)
ZHAO YONGXIANG (CN)
HE KONGWANG (CN)
NI YANXIU (CN)
CHANG XINJIAN (CN)
SHI DANYI (CN)
Application Number:
PCT/CN2021/073807
Publication Date:
November 04, 2021
Filing Date:
January 26, 2021
Export Citation:
Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
G01N33/577; G01N33/558; G01N33/569
Domestic Patent References:
| WO2007053165A2 | 2007-05-10 | |||
| WO2006076007A2 | 2006-07-20 |
Foreign References:
| CN111596064A | 2020-08-28 | |||
| KR20050057750A | 2005-06-16 | |||
| CN109232736A | 2019-01-18 | |||
| CN105527437A | 2016-04-27 | |||
| CN101339192A | 2009-01-07 | |||
| CN110346554A | 2019-10-18 | |||
| CN105606802A | 2016-05-25 | |||
| CN103033626A | 2013-04-10 | |||
| KR20080040942A | 2008-05-09 |
Attorney, Agent or Firm:
BEIJING NEWIPS INTELLECTUAL PROPERTY LTD. (CN)
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