Title:
RAPMAP METHOD FOR RAPID AND HIGH-THROUGHPUT POSITIONING AND CLONING OF PLANT QTL GENE
Document Type and Number:
WIPO Patent Application WO/2021/196255
Kind Code:
A1
Abstract:
Disclosed is a RapMap method for rapid and high-throughput positioning and cloning of a plant QTL gene. The method comprises: selecting strains having relatively small character differences from core germplasm as gradient parents, constructing as many F2 gradient genetic populations as possible, preliminarily positioning QTL by performing chip detection or next-generation sequencing on two separated extreme phenotypic DNA pools of each population, verifying the QTL at the single plant level of each gradient population according to "co-separation criteria" provided by the present invention, and then using a QTL heterozygous family as a near-isogenic line for fine positioning to clone a target gene.
Inventors:
LI YIBO (CN)
ZHANG JUNCHENG (CN)
ZHANG DEJIAN (CN)
LI CUICUI (CN)
XU PENGKUN (CN)
FAN YAWEI (CN)
LI WEI (CN)
SU XIAOMIN (CN)
SUN QI (CN)
LI XINGXING (CN)
SONG YINGYING (CN)
ZHANG JUNCHENG (CN)
ZHANG DEJIAN (CN)
LI CUICUI (CN)
XU PENGKUN (CN)
FAN YAWEI (CN)
LI WEI (CN)
SU XIAOMIN (CN)
SUN QI (CN)
LI XINGXING (CN)
SONG YINGYING (CN)
Application Number:
PCT/CN2020/083758
Publication Date:
October 07, 2021
Filing Date:
April 08, 2020
Export Citation:
Assignee:
UNIV HUAZHONG AGRICULTURAL (CN)
International Classes:
G16B20/40; G16B20/20
Domestic Patent References:
| WO2013166195A2 | 2013-11-07 |
Foreign References:
| CN103642801A | 2014-03-19 | |||
| CN109326322A | 2019-02-12 |
Other References:
WU JIANHUI; HUANG SHUO; ZENG QINGDONG; LIU SHENGJIE; WANG QILIN; MU JINGMEI; YU SHIZHOU; HAN DEJUN; KANG ZHENSHENG: "Comparative genome-wide mapping versus extreme pool-genotyping and development of diagnostic SNP markers linked to QTL for adult plant resistance to stripe rust in common wheat", THEORETICAL AND APPLIED GENETICS ; INTERNATIONAL JOURNAL OF PLANT BREEDING RESEARCH, SPRINGER, BERLIN, DE, vol. 131, no. 8, 16 June 2018 (2018-06-16), Berlin, DE, pages 1777 - 1792, XP036549892, ISSN: 0040-5752, DOI: 10.1007/s00122-018-3113-7
DENG YINGDE, CENG-LIN XIAO, HUA-BING DENG, HAI-QING ZHANG, DENG XIAO-JUAN, LIU YU-LONG: "Detection of QTL Related to Stigma Exsertion Rate (SER) in Rice (Oryza sativa L .) by Bulked Segregant Analysis", RESEARCH OF AGRICULTURAL MODERNIZATION, vol. 32, no. 2, 15 March 2011 (2011-03-15), pages 230 - 233, XP055855188, ISSN: 1000-0275
DENG YINGDE, CENG-LIN XIAO, HUA-BING DENG, HAI-QING ZHANG, DENG XIAO-JUAN, LIU YU-LONG: "Detection of QTL Related to Stigma Exsertion Rate (SER) in Rice (Oryza sativa L .) by Bulked Segregant Analysis", RESEARCH OF AGRICULTURAL MODERNIZATION, vol. 32, no. 2, 15 March 2011 (2011-03-15), pages 230 - 233, XP055855188, ISSN: 1000-0275
Attorney, Agent or Firm:
WUHAN ZHI JIA JOINT INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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