Title:
REACTION SYSTEM FOR PREPARING DNA NANOBALL AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/098178
Kind Code:
A1
Abstract:
Provided is a reaction system for preparing a DNA nanoball (DNB), comprising: adapters; an enzyme, wherein the enzyme comprises a DNA ligase; and a buffer solution. The adapters comprise an adapter A and an adapter B. The adapter A comprises a first strand and a second strand. The first strand comprises a sequence a, and the second strand comprises a sequence a' and a sequence b. The sequence a and the sequence a' are complementary and paired. The adapter B comprises a third strand and a fourth strand. The third strand comprises a sequence c and a sequence e, and the fourth strand comprises a sequence e', a sequence f, and a sequence b'. The sequence b' and the sequence c are complementary and paired. The sequence e and the sequence e' are complementary and paired. The sequence b and the sequence b' are complementary and paired.
Inventors:
KONG GEZHI (CN)
XIA JUN (CN)
YANG LIN (CN)
ZHANG YANYAN (CN)
XIA JUN (CN)
YANG LIN (CN)
ZHANG YANYAN (CN)
Application Number:
PCT/CN2022/130243
Publication Date:
May 16, 2024
Filing Date:
November 07, 2022
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
International Classes:
C12N15/11; C12Q1/68; C40B40/06
Domestic Patent References:
| WO2020113460A1 | 2020-06-11 | |||
| WO2016078095A1 | 2016-05-26 | |||
| WO2022141061A1 | 2022-07-07 |
Foreign References:
| CN113249457A | 2021-08-13 | |||
| CN112111485A | 2020-12-22 | |||
| CN111286527A | 2020-06-16 | |||
| CN113502287A | 2021-10-15 |
Other References:
CHEN, Y. ET AL.: "Reusable and Sensitive Exonuclease III Activity Detection on DNB Nanoarrays Based on CPAS Sequencing Technology.", ENZYME AND MICROBIAL TECHNOLOGY., vol. 150, 26 July 2021 (2021-07-26), XP086765730, DOI: 10.1016/j.enzmictec.2021.109878
Attorney, Agent or Firm:
FASHENG INTELLECTUAL PROPERTY COMPANY, LTD. (CN)
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