Title:
RECOMBINANT ADENOVIRUS COMPRISING HTERT MRNA TARGETING RIBOZYME AND P53 GENE
Document Type and Number:
WIPO Patent Application WO/2014/062004
Kind Code:
A1
Abstract:
The present invention relates to: a recombinant adenovirus; a host cell into which the recombinant adenovirus is introduced; a pharmaceutical composition comprising the recombinant adenovirus as an active ingredient for preventing or treating cancers; and a method for treating cancers comprising the step of administering the pharmaceutical composition including the recombinant adenovirus as an active ingredient to a subject in need of treatment at a pharmaceutically effective amount, wherein, the recombinant adenovirus comprises a promoter, a ribozyme which is targeted to human telomerase reverse transcriptase (hTERT) mRNA, and a p53 gene in an adenovirus backbone of which E1 and E3 are deleted. The recombinant adenovirus of the present invention inhibits the activity of hTERT mRNA, which is specifically overexpressed in cancer cells, by cutting hTERT mRNA through a trans-splicing ribozyme, and simultaneously allows p53, which is a therapeutic gene, to be expressed. Thus it is confirmed that cancer cells are effectively killed using the recombinant adenovirus of the present invention. Particularly, it is confirmed that liver tissue-specific migration, particularly selective motility toward cancer cells, is improved. Thus, since the recombinant adenovirus of the present invention does not show cell cytotoxicity to normal cells for systemic administration by having improved selective motility, the present invention can be used as a gene therapeutic agent for hepatoma cancer or metastatic liver cancer.
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Inventors:
LEE WON SUP (KR)
KIM IN HOO (KR)
LEE SANG JIN (KR)
KIM IN HOO (KR)
LEE SANG JIN (KR)
Application Number:
PCT/KR2013/009264
Publication Date:
April 24, 2014
Filing Date:
October 16, 2013
Export Citation:
Assignee:
NAT UNIV GYEONGSANG IACF (KR)
International Classes:
C12N7/01; A61K35/76; A61K35/761; A61P35/00
Foreign References:
| KR20100024055A | 2010-03-05 | |||
| US20100034775A1 | 2010-02-11 |
Other References:
JIN-SOOK JEONG ET AL.: "Antitumor effects of systemically delivered adenovirus harboring trans-splicing ribozyme in intrahepatic colon cancer mouse model.", CLINICAL CANCER RESEARCH., vol. 14, no. 1, 2008, pages 1 - 7
PARK, GI SEO ET AL.: "Role of Adenovirus in Gene Therapy and Vaccine.", BIOWAVE., vol. 8, no. 10, 2006, pages 281 - 290
PARK, GI SEO ET AL.: "Role of Adenovirus in Gene Therapy and Vaccine.", BIOWAVE., vol. 8, no. 10, 2006, pages 281 - 290
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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