Title:
RECOMBINANT CELLS PRODUCING ITACONIC ACID AND METHYL ESTERS THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/181312
Kind Code:
A3
Abstract:
The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid,4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which over expresses: -a nucleic 1 acid encoding a polypeptide having cis-aconitate decarboxylase activity; and-one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA,wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans- aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
Inventors:
ZHAO ZHENG (NL)
MEIJRINK BERNARD (NL)
HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT (NL)
WU LIANG (NL)
ROUBOS JOHANNES ANDRIES (NL)
GELDER VAN MARINA (NL)
MEIJRINK BERNARD (NL)
HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT (NL)
WU LIANG (NL)
ROUBOS JOHANNES ANDRIES (NL)
GELDER VAN MARINA (NL)
Application Number:
PCT/EP2015/061884
Publication Date:
January 21, 2016
Filing Date:
May 28, 2015
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
International Classes:
C12N15/52; C12P7/46; C12P7/62
Domestic Patent References:
| WO2013144257A1 | 2013-10-03 | |||
| WO2014080024A2 | 2014-05-30 |
Foreign References:
| US20100285546A1 | 2010-11-11 | |||
| US20110053232A1 | 2011-03-03 |
Other References:
CAI, H. ET AL.: "Identification of the Gene and Characterization of the Activity of the trans-Aconitate Methyltransferase from Saccharomyces cerevisiae", BIOCHEMISTRY, vol. 40, no. 45, 19 October 2001 (2001-10-19), pages 13699 - 13709, XP002743558
Attorney, Agent or Firm:
MONACO, Vania (P.O. Box 4, AA Echt, NL)
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