Title:
RECOMBINANT CELLULOSE DIASTATIC ENZYME COCKTAIL, RECOMBINANT YEAST COMPLEX STRAIN, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/002471
Kind Code:
A1
Abstract:
The present invention relates to: an expression cassette which has an increased protein secretion in yeast compared to wild-type and which comprises a polynucleotide for coding a translational fusion partner (TFP) and a polynucleotide for coding a protein selected from the group consisting of: TrXynII, TrBx1, TrEGL2, PaCEL1, PaCel2, TeCBH1, NfCBH1, HgCBH1, CtCBH1, ClCBH2, and CfCex1; an expression vector comprising the same; a transformant in which the expression vector is introduced into a host cell; and a complex strain comprising two or more of the transformants. In addition, the present invention relates to a method for producing hemicellulase, endoglucanase, and exoglucanase, or a method for producing bioethanol, comprising a step for culturing the transformants. Furthermore, the present invention relates to a cellulase cocktail comprising endoglucanase and exoglucanase produced by the method and beta-glucosidase, and a method for saccharifying biomass using the cocktail. Moreover, the prevent invention relates to a method for producing bioenergy or useful biochemicals from cellulosic biomass, using a single strain which produces the cellulase, or two or more strains in combination.
Inventors:
SOHN JUNG HOON (KR)
SUNG BONG HYUN (KR)
BAE JUNG HOON (KR)
LEE CHO RYONG (KR)
KIM MI JIN (KR)
KIM HYUN JIN (KR)
YONG HWAN UNG (KR)
LIM KWANG MOOK (KR)
SUNG BONG HYUN (KR)
BAE JUNG HOON (KR)
LEE CHO RYONG (KR)
KIM MI JIN (KR)
KIM HYUN JIN (KR)
YONG HWAN UNG (KR)
LIM KWANG MOOK (KR)
Application Number:
PCT/KR2014/005926
Publication Date:
January 08, 2015
Filing Date:
July 02, 2014
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
International Classes:
C12N15/81; C12N15/62; C12P7/06
Foreign References:
| KR20120140129A | 2012-12-28 | |||
| KR20120116765A | 2012-10-23 |
Other References:
DATABASE GENBANK 1 May 2013 (2013-05-01), "Endoglucanase EG -II [Trichoderma reesei]", accession no. 07982.1
YAMADA, RYOUSKE ET AL.: "Direct ethanol production from cellulosic materials using a diploid strain of Saccharomycess cerevisiae with optimized cellulase expression", BIOTECHNOLOGY FOR BIOFUELS, vol. 4, no. 8, 2011, pages 1 - 8
DATABASE GENBANK "Endo-1,4-beta-xylanase 2 [Trichoderma reesei", accession no. 36217.1
DATABASE GENBANK 19 March 2012 (2012-03-19), "beta-xylosidase [Trichoderma orientale", accession no. FD50200.1
YAMADA, RYOUSKE ET AL.: "Direct ethanol production from cellulosic materials using a diploid strain of Saccharomycess cerevisiae with optimized cellulase expression", BIOTECHNOLOGY FOR BIOFUELS, vol. 4, no. 8, 2011, pages 1 - 8
DATABASE GENBANK "Endo-1,4-beta-xylanase 2 [Trichoderma reesei", accession no. 36217.1
DATABASE GENBANK 19 March 2012 (2012-03-19), "beta-xylosidase [Trichoderma orientale", accession no. FD50200.1
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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