RECOMBINANT DNA HAVING HYDANTOINASE GENE AND CARBAMYLASE GENE AND PROCESS FOR PRODUCING AMINO ACID

Title:

RECOMBINANT DNA HAVING HYDANTOINASE GENE AND CARBAMYLASE GENE AND PROCESS FOR PRODUCING AMINO ACID

Document Type and Number:

WIPO Patent Application WO/2003/085108

Kind Code:

A1

Abstract:

It is intended to construct a recombinant DNA whereby enzyme genes such as HHase genes can be efficiently co-expressed and to efficiently produce an amino acid from hydantoin using such a recombinant DNA. Namely, a recombinant DNA into which base sequences respectively encoding the following gene regions (I) and (II) are integrated in a definite direction in a definite order can be provided and an amino acid can be efficiently produced. (I) A gene region which contains a base sequence encoding a hydantoinase gene and a trp promoter sequence being located in the upstream of the base sequence encoding the hydantoinase gene and controlling the expression of the hydantoinase gene. (II) A gene region which contains a base sequence encoding a carbamylase gene and a trp promoter sequence being located in the upstream of the base sequence encoding the carbamylase gene and controlling the expression of the carbamylase gene.

Inventors:

KIRA IKUO (JP)
TAKENAKA YASUHIRO (JP)
NOZAKI HIROYUKI (JP)
WATANABE KUNIHIKO (JP)

Application Number:

PCT/JP2003/004553

Publication Date:

October 16, 2003

Filing Date:

April 10, 2003

Export Citation:

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Assignee:

AJINOMOTO KK (JP)
KIRA IKUO (JP)
TAKENAKA YASUHIRO (JP)
NOZAKI HIROYUKI (JP)
WATANABE KUNIHIKO (JP)

International Classes:

C12N1/21; C12N9/80; C12N9/86; C12N15/71; C12P13/04; (IPC1-7): C12N15/09; C12N1/21; C12N9/78; C12N9/86; C12N9/90

Domestic Patent References:

WO2001023582A1	2001-04-05
WO2000058449A1	2000-10-05
WO2001078095A1	2001-10-18

Foreign References:

US5708148A	1998-01-13
US5834258A	1998-11-10
EP1275723A1	2003-01-15
US20030113880A1	2003-06-19
JP2002330784A	2002-11-19
JP2002330785A	2002-11-19

Other References: