Title:
RECOMBINANT E. COLI SYSTEM, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF IN SYNTHESIS OF α-1,2-FUCOSYLATED OLIGOSACCHARIDE
Document Type and Number:
WIPO Patent Application WO/2021/093022
Kind Code:
A1
Abstract:
The present invention relates to the technical field of genetic engineering, and provides a recombinant E. coli system, a construction method therefor, and an application thereof in the synthesis of an α-1,2-fucosylated oligosaccharide. The recombinant E. coli system has an α-1,2-fucosyltransferase gene and a GDP-fucose pyrophosphorylase gene. By transforming the α-1,2-fucosyltransferase gene and the GDP-fucose pyrophosphorylase gene into E. coli, a genetically engineered strain is constructed to express an α-1,2-fucosyltransferase and a GDP-fucose pyrophosphorylase, and the expressed α-1,2-fucose pyrophosphorylase and GDP-fucose pyrophosphorylase are used for enzymatic synthesis of an α-1,2-fucosylated oligosaccharide.
Inventors:
HUANG JIN (CN)
XUE XIAOZHOU (CN)
LI YU (CN)
QIN HUIMIN (CN)
TONG YI (CN)
LI YI (CN)
CHEN BO (CN)
LI FAN (CN)
AN TAI (CN)
XUE XIAOZHOU (CN)
LI YU (CN)
QIN HUIMIN (CN)
TONG YI (CN)
LI YI (CN)
CHEN BO (CN)
LI FAN (CN)
AN TAI (CN)
Application Number:
PCT/CN2019/120873
Publication Date:
May 20, 2021
Filing Date:
November 26, 2019
Export Citation:
Assignee:
NUTRITION & HEALTH RES INSTITUTE COFCO CORPORATION (CN)
COFCO JILIN BIO CHEMICAL TECH CO LTD (CN)
COFCO JILIN BIO CHEMICAL TECH CO LTD (CN)
International Classes:
C12N15/70; C12N9/10; C12P19/02
Foreign References:
| KR101648352B1 | 2016-08-16 | |||
| CN108611384A | 2018-10-02 |
Other References:
CHIN ET AL: "Metabolic engineering of Escherichia coli to produce 2'-fucosyllactose via salvage pathway of guanosine 5'-diphosphate (GDP)-L-fucose", BIOTECHNOLOGY AND BIOENGINEERING, vol. 113, 20 June 2016 (2016-06-20), pages 2443 - 2452, XP002765455
W. WANG, T. HU, P. A. FRANTOM, T. ZHENG, B. GERWE, D. S. DEL AMO, S. GARRET, R. D. SEIDEL, P. WU: "Chemoenzymatic synthesis of GDP-L-fucose and the Lewis X glycan derivatives", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 38, 22 September 2009 (2009-09-22), pages 16096 - 16101, XP055093077, ISSN: 00278424, DOI: 10.1073/pnas.0908248106
DATABASE PROTEIN 9 November 2018 (2018-11-09), ANONYMOUS: "kinase [Bacteroides fragilis]", XP055811930, retrieved from NCBI Database accession no. WP_010993080
DATABASE NUCLEOTIDE 31 January 2014 (2014-01-31), ANONYMOUS: "Helicobacter pylori HPAG1, complete genome", XP055811937, retrieved from NCBI Database accession no. CP000241
MATTILA P, ET AL.: "FUNCTIONAL EXPRESSION OF ESCHERICHIA COLI ENZYMES SYNTHESIZING GDP-L-FUCOSE FROM INHERENT GDP-D-MANNOSE IN SACCHAROMYCES CEREVISIAE", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 10, no. 10, 1 October 2000 (2000-10-01), US, pages 1041 - 1047, XP008023410, ISSN: 0959-6658, DOI: 10.1093/glycob/10.10.1041
W. WANG, T. HU, P. A. FRANTOM, T. ZHENG, B. GERWE, D. S. DEL AMO, S. GARRET, R. D. SEIDEL, P. WU: "Chemoenzymatic synthesis of GDP-L-fucose and the Lewis X glycan derivatives", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 38, 22 September 2009 (2009-09-22), pages 16096 - 16101, XP055093077, ISSN: 00278424, DOI: 10.1073/pnas.0908248106
DATABASE PROTEIN 9 November 2018 (2018-11-09), ANONYMOUS: "kinase [Bacteroides fragilis]", XP055811930, retrieved from NCBI Database accession no. WP_010993080
DATABASE NUCLEOTIDE 31 January 2014 (2014-01-31), ANONYMOUS: "Helicobacter pylori HPAG1, complete genome", XP055811937, retrieved from NCBI Database accession no. CP000241
MATTILA P, ET AL.: "FUNCTIONAL EXPRESSION OF ESCHERICHIA COLI ENZYMES SYNTHESIZING GDP-L-FUCOSE FROM INHERENT GDP-D-MANNOSE IN SACCHAROMYCES CEREVISIAE", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 10, no. 10, 1 October 2000 (2000-10-01), US, pages 1041 - 1047, XP008023410, ISSN: 0959-6658, DOI: 10.1093/glycob/10.10.1041
Attorney, Agent or Firm:
RUNPING & PARTNERS (CN)
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