Title:
RECOMBINANT ESCHERICHIA COLI FOR EFFICIENT PRODUCTION OF SUCCINIC ACID AND CONSTRUCTION METHOD FOR RECOMBINANT ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2022/088263
Kind Code:
A1
Abstract:
Disclosed are recombinant Escherichia coli for efficient production of succinic acid and a construction method for the recombinant Escherichia coli. The method comprises: knocking out a by-product encoding gene of a succinic acid producing strain E.coli FMME-N-5 to obtain a strain E.coli FMME-N-5(ΔfocA-pflB-ΔldhA-Δpta-ackA); and introducing a plasmid pTrcHisA-pck-ptxD into the strain, overexpressing actinobacillus succinogenes phosphoenolpyruvate carboxykinase pck and bacillus stutzeri phosphite dehydrogenase ptxD, and screening to obtain an engineering bacterium E.coli FMME-N-5(ΔfocA-pflB-ΔldhA-Δpta-ackA)-pck-ptxD for efficient production of succinic acid, the engineering strain using a two-stage fermentation strategy on a 7.5 L fermentation tank for fermentation for 96 h, the succinic acid yield reaching 137 g/L, the yield of succinic acid reaching 1 g/g glucose, the production strength being 1.43 g/L/h, by-products lactic acid and formic acid being not accumulated, and acetic acid being 1-2 g/L.
Inventors:
LIU LIMING (CN)
TANG WENXIU (CN)
SHEN CHEN (CN)
LUO QIULING (CN)
CHEN XIULAI (CN)
LIU JIA (CN)
GAO CONG (CN)
SONG WEI (CN)
TANG WENXIU (CN)
SHEN CHEN (CN)
LUO QIULING (CN)
CHEN XIULAI (CN)
LIU JIA (CN)
GAO CONG (CN)
SONG WEI (CN)
Application Number:
PCT/CN2020/128254
Publication Date:
May 05, 2022
Filing Date:
November 12, 2020
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12N15/53; C12N15/54; C12N15/60; C12N15/70; C12P7/46; C12R1/19
Domestic Patent References:
| WO2011063157A2 | 2011-05-26 |
Foreign References:
| CN105779513A | 2016-07-20 | |||
| CN105658801A | 2016-06-08 | |||
| CN104974946A | 2015-10-14 | |||
| CN104694449A | 2015-06-10 | |||
| CN102282156A | 2011-12-14 |
Other References:
YUE FANGFANG, JIANG MIN, MA JIANGFENG, YU LI, LIU SHUWEN, WEI PING: "Construction of engineered Escherichia coli for succinate producition", CHINA BREWING, ZHONGGUO NIANGZAO BIANJIBU, CN, no. 2, 15 February 2010 (2010-02-15), CN , pages 25 - 29, XP055926579, ISSN: 0254-5071
YU YONG; ZHU XINNA; XU HONGTAO; ZHANG XUELI: "Construction of an energy-conserving glycerol utilization pathways for improving anaerobic succinate production in Escherichia coli", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 56, 7 October 2019 (2019-10-07), AMSTERDAM, NL, pages 181 - 189, XP085876192, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2019.10.002
YU YONG; ZHU XINNA; XU HONGTAO; ZHANG XUELI: "Construction of an energy-conserving glycerol utilization pathways for improving anaerobic succinate production in Escherichia coli", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 56, 7 October 2019 (2019-10-07), AMSTERDAM, NL, pages 181 - 189, XP085876192, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2019.10.002
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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