Title:
RECOMBINANT EXPRESSION GLUTAMATE OXIDASE
Document Type and Number:
WIPO Patent Application WO/2023/140286
Kind Code:
A1
Abstract:
This invention produces glutamate oxidase that does not need to be treated with a protease. Provided is a glutamate oxidase mutant obtained by deleting a designated amino acid sequence.
Inventors:
KUZE KEITARO (JP)
ICHIYANAGI ATSUSHI (JP)
HIZUME TATSUYA (JP)
ICHIYANAGI ATSUSHI (JP)
HIZUME TATSUYA (JP)
Application Number:
PCT/JP2023/001336
Publication Date:
July 27, 2023
Filing Date:
January 18, 2023
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12N15/63; C12P21/02
Domestic Patent References:
| WO2021193598A1 | 2021-09-30 |
Foreign References:
| CN109266664A | 2019-01-25 | |||
| CN106282205A | 2017-01-04 |
Other References:
DATABASE PROTEIN 25 December 2021 (2021-12-25), ANONYMOUS : "L-glutamate oxidase [Streptomyces sp.]", XP093080079, retrieved from GENBANK Database accession no. BCH42006
ARIMA JIRO,ET AL: "Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6.", JOURNAL OF BIOCHEMISTRY, vol. 134, no. 6, 1 December 2003 (2003-12-01), GB , pages 805 - 812, XP002273495, ISSN: 0021-924X, DOI: 10.1093/jb/mvg206
SUGIMORI, DAISUKE; HAYASHI, YUKA; SAITO, TAKAHIRO; KUBOTA, HITOMI; SAKASEGAWA, SHIN-ICHI; NAKANO, SYOGO; ITO, SOHEI; ASANO, YASUHI: "4E1a07 Enhancing the thermostability of L-glutamate oxidase from Streptomyces sp. NT1 by artificial protein design", ANNUAL MEETING OF THE JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2019 (JSBBA 2019), vol. 2019, 5 March 2019 (2019-03-05), pages 1675, XP009547825
HAYASHI, YUKA; NAKAMUA, MASAYA; SAKASEGAWA, SHIN-ICHI; NAKANO, SHOGO; ITO, SOHEI; ASANO, YASUHISA; SUGIMORI, DAISUKE: "2A25p06 Streptomyces sp. Improvement of heat resistance by artificial protein design of L-glutamate oxidase derived from strain NT1", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY (JSBBA), vol. 2018, 5 March 2018 (2018-03-05), pages 814, XP009547826
HAYASHI YUKA, NAKAMURA MASAYA, NAKANO SHOGO, ITO SOHEI, ASANO YASUHISA, SUGIMORI DAISUKE: "Thermostability enhancement of l-glutamate oxidase from Streptomyces sp. NT1 by full consensus protein design", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 133, no. 4, 1 April 2022 (2022-04-01), NL , pages 309 - 315, XP093080105, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2021.12.008
ARIMA JIRO,ET AL: "Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6.", JOURNAL OF BIOCHEMISTRY, vol. 134, no. 6, 1 December 2003 (2003-12-01), GB , pages 805 - 812, XP002273495, ISSN: 0021-924X, DOI: 10.1093/jb/mvg206
SUGIMORI, DAISUKE; HAYASHI, YUKA; SAITO, TAKAHIRO; KUBOTA, HITOMI; SAKASEGAWA, SHIN-ICHI; NAKANO, SYOGO; ITO, SOHEI; ASANO, YASUHI: "4E1a07 Enhancing the thermostability of L-glutamate oxidase from Streptomyces sp. NT1 by artificial protein design", ANNUAL MEETING OF THE JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2019 (JSBBA 2019), vol. 2019, 5 March 2019 (2019-03-05), pages 1675, XP009547825
HAYASHI, YUKA; NAKAMUA, MASAYA; SAKASEGAWA, SHIN-ICHI; NAKANO, SHOGO; ITO, SOHEI; ASANO, YASUHISA; SUGIMORI, DAISUKE: "2A25p06 Streptomyces sp. Improvement of heat resistance by artificial protein design of L-glutamate oxidase derived from strain NT1", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY (JSBBA), vol. 2018, 5 March 2018 (2018-03-05), pages 814, XP009547826
HAYASHI YUKA, NAKAMURA MASAYA, NAKANO SHOGO, ITO SOHEI, ASANO YASUHISA, SUGIMORI DAISUKE: "Thermostability enhancement of l-glutamate oxidase from Streptomyces sp. NT1 by full consensus protein design", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 133, no. 4, 1 April 2022 (2022-04-01), NL , pages 309 - 315, XP093080105, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2021.12.008
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
Download PDF: