Title:
RECOMBINANT LACTOCOCCUS LACTIS, MICROCAPSULE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/141744
Kind Code:
A1
Abstract:
Provided are recombinant Lactococcus lactis, a microcapsule and the use thereof, which belong to the technical field of genetic engineering. A first fusion gene or second fusion gene is inserted into PNZ8149 by means of using PNZ8149 as a basic vector, wherein the first fusion gene comprises signal peptide SPusp45, probiotic LEISS, enterokinase recognition site DDDDK and human ethanol dehydrogenase connected in series in sequence, and the second fusion gene comprises signal peptide SPusp45, probiotic LEISS, enterokinase recognition site DDDDK and human acetaldehyde dehydrogenase connected in series in sequence. A target enzyme is used to separate the signal peptide sequence SPusp45 and the LEISS sequence from a fusion protein by means of enzyme digestion under the action of endogenous enterokinase in the intestine, and at the same time, free ethanol dehydrogenase or acetaldehyde dehydrogenase is released, and the released ethanol dehydrogenase or acetaldehyde dehydrogenase can decompose ethanol.
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Inventors:
JIN WANZHU (CN)
JIANG XIAOXIAO (CN)
YAN CHUNLONG (CN)
JIANG XIAOXIAO (CN)
YAN CHUNLONG (CN)
Application Number:
PCT/CN2022/073656
Publication Date:
August 03, 2023
Filing Date:
January 25, 2022
Export Citation:
Assignee:
INST ZOOLOGY CAS (CN)
International Classes:
C12N1/21; A23L33/135; A61K9/50; A61K35/744; A61P39/02; C12N15/62; C12N15/74
Domestic Patent References:
| WO2019195592A1 | 2019-10-10 |
Foreign References:
| CN112210519A | 2021-01-12 | |||
| CN112175890A | 2021-01-05 | |||
| CN111500615A | 2020-08-07 | |||
| CN111518801A | 2020-08-11 |
Other References:
LYU YUNBIN, ZHONG LEI, LIU YANAN, LU JING, LAPOINTE GISÈLE, LU FENGXIA, LU ZHAOXIN: "Protective effects of Lactococcus lactis expressing alcohol dehydrogenase and acetaldehyde dehydrogenase on acute alcoholic liver injury in mice : Protective effects of Lactococcus lactis expressing alcohol", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, WILEY, HOBOKEN, USA, vol. 93, no. 5, 1 May 2018 (2018-05-01), Hoboken, USA, pages 1502 - 1510, XP093083189, ISSN: 0268-2575, DOI: 10.1002/jctb.5521
ZHANG DANYU, CHEN YANG-YANG; LIU XIAO-YANG; WANG JIA-XING; ZHANG WEI-CAI; BU NING; XIONG XIANG-HUA: "Expression and Purification of Acetaldehyde Dehydrogenase in Lactococcus lactis", LETTERS IN BIOTECHNOLOGY, JUNSHI YIXUE KEXUEYUAN, CN, vol. 31, no. 1, 31 January 2020 (2020-01-31), CN , pages 39 - 43, XP093083193, ISSN: 1009-0002, DOI: 10.3969/j.issn.1009-0002.2020.01.008
MA CHAO, ET AL: "Reconstruction of a Food-grade Expression Vector in Lactococcus lactis", ANHUI NONGYE KEXUE (JOURNAL OF ANHUI AGRICULTURAL SCIENCES), ANHUI SHENG NONGYE KEXUEYUAN, CN, vol. 38, no. 19, 31 December 2010 (2010-12-31), CN , pages 10247 - 10250, XP093083201, ISSN: 0517-6611, DOI: 10.13989/j.cnki.0517-6611.2010.19.058
ZHANG DANYU, CHEN YANG-YANG; LIU XIAO-YANG; WANG JIA-XING; ZHANG WEI-CAI; BU NING; XIONG XIANG-HUA: "Expression and Purification of Acetaldehyde Dehydrogenase in Lactococcus lactis", LETTERS IN BIOTECHNOLOGY, JUNSHI YIXUE KEXUEYUAN, CN, vol. 31, no. 1, 31 January 2020 (2020-01-31), CN , pages 39 - 43, XP093083193, ISSN: 1009-0002, DOI: 10.3969/j.issn.1009-0002.2020.01.008
MA CHAO, ET AL: "Reconstruction of a Food-grade Expression Vector in Lactococcus lactis", ANHUI NONGYE KEXUE (JOURNAL OF ANHUI AGRICULTURAL SCIENCES), ANHUI SHENG NONGYE KEXUEYUAN, CN, vol. 38, no. 19, 31 December 2010 (2010-12-31), CN , pages 10247 - 10250, XP093083201, ISSN: 0517-6611, DOI: 10.13989/j.cnki.0517-6611.2010.19.058
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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