Title:
RECOMBINANT LENTIVIRUS THAT EFFECTIVELY INTERFERES WITH EXPRESSION OF TAU PROTEIN, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/253729
Kind Code:
A1
Abstract:
Provided is a shRNA, comprising a sequence represented by SEQ ID NO.8 and a reverse complementary sequence of SEQ ID NO.8. Also provided are a double-stranded oligonucleotide comprising the SEQ ID NO.1 sequence, a double-stranded oligonucleotide formed by complementary base pairing of single-stranded oligonucleotides represented by sequences such as SEQ ID NO.3 and SEQ ID NO.4, a recombinant lentivirus carrying said double-stranded oligonucleotide and the RNA form thereof, and the use thereof. The shRNA can be cleaved within a cell to form siRNA that interferes with the expression of Tau protein.
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Inventors:
CEN XIAOBO (CN)
LI HONGCHUN (CN)
LI HONGCHUN (CN)
Application Number:
PCT/CN2020/132402
Publication Date:
December 23, 2021
Filing Date:
November 27, 2020
Export Citation:
Assignee:
WEST CHINA HOSPITAL SICHUAN UNIV (CN)
International Classes:
C12N15/113; A61K31/712; A61P25/28
Foreign References:
| CN101448944A | 2009-06-03 | |||
| CN109072237A | 2018-12-21 | |||
| EP3655013A1 | 2020-05-27 |
Other References:
CHEN JONATHAN L., ZHANG PEIYUAN, ABE MASAHITO, AIKAWA HARUO, ZHANG LIYING, FRANK ALEXANDER J., ZEMBRYSKI TIMOTHY, HUBBS CHRISTOPHE: "Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 142, no. 19, 13 May 2020 (2020-05-13), pages 8706 - 8727, XP055792632, ISSN: 0002-7863, DOI: 10.1021/jacs.0c00768
XIA ZHAOYUN, ZHAO HONG, LUCY PAN, SHI YANG, LU ZHENGYU: "Research progress on the role of tau protein in Alzheimer's disease", NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION, vol. 14, no. 9, 30 September 2019 (2019-09-30), CN , pages 450 - 453, XP009533168, ISSN: 1001-117X, DOI: 10.16780/j.cnki.sjssgncj.2019.09.006
SARAH L. DEVOS ET AL.: "Tau reduction prevents neuronal loss and reverses pathological tau deposition and seeding in mice with tauopathy", SCIENCE TRANSLATIONAL MEDICINE, vol. 9, 25 January 2017 (2017-01-25), XP055702410, ISSN: 1946-6242
XIA ZHAOYUN, ZHAO HONG, LUCY PAN, SHI YANG, LU ZHENGYU: "Research progress on the role of tau protein in Alzheimer's disease", NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION, vol. 14, no. 9, 30 September 2019 (2019-09-30), CN , pages 450 - 453, XP009533168, ISSN: 1001-117X, DOI: 10.16780/j.cnki.sjssgncj.2019.09.006
SARAH L. DEVOS ET AL.: "Tau reduction prevents neuronal loss and reverses pathological tau deposition and seeding in mice with tauopathy", SCIENCE TRANSLATIONAL MEDICINE, vol. 9, 25 January 2017 (2017-01-25), XP055702410, ISSN: 1946-6242
Attorney, Agent or Firm:
GAOYUNG INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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