Title:
RECOMBINANT MICROORGANISM, PREPARATION METHOD THEREFOR, AND APPLICATION OF RECOMBINANT MICROORGANISM IN PRODUCTION OF TAGATOSE
Document Type and Number:
WIPO Patent Application WO/2022/095684
Kind Code:
A1
Abstract:
Provided are a recombinant microorganism modified by means of genetic engineering and an application of the recombinant microorganism in production of tagatose, a preparation method for the recombinant microorganism, a tagatose production strain and a tagatose production method. According to the recombinant microorganism, the tagatose is produced by taking glucose as a substrate or taking glycerol and glucose as substrates; the efficiency of conversion and production of tagatose by means of the recombinant microorganism is high, and a multi-enzyme purification process step is not needed.
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Inventors:
MA YANHE (CN)
SHI TING (CN)
SONG YUNHONG (CN)
ZHANG TING (CN)
LI YUNJIE (CN)
SHI TING (CN)
SONG YUNHONG (CN)
ZHANG TING (CN)
LI YUNJIE (CN)
Application Number:
PCT/CN2021/124427
Publication Date:
May 12, 2022
Filing Date:
October 18, 2021
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/21; C12N1/19; C12P19/02; C12R1/125; C12R1/15; C12R1/19; C12R1/225; C12R1/865
Domestic Patent References:
| WO2018182355A1 | 2018-10-04 | |||
| WO2020067649A1 | 2020-04-02 | |||
| WO2015016544A1 | 2015-02-05 | |||
| WO2018004310A1 | 2018-01-04 |
Foreign References:
| CN112342178A | 2021-02-09 | |||
| CN109666620A | 2019-04-23 | |||
| CN101189332A | 2008-05-28 | |||
| CN105916389A | 2016-08-31 | |||
| CN109790524A | 2019-05-21 | |||
| CN109415715A | 2019-03-01 | |||
| CN106399427A | 2017-02-15 |
Other References:
OH D-K: "Tagatose: properties, applications, and biotechnological processes", APP. MICROBIOL. BIOTECHNOL., vol. 76, 2007, pages 1 - 8, XP019538798, DOI: 10.1007/s00253-007-0981-1
RHIMI MAGHAJARI NJUY MCHOUAYEKH HMAGUIN EHASER RBEJAR S: "Rational design of Bacillus stearothermophilus US 1001-arabinose isomerase: Potential applications for d-tagatose production", BIOCHIM., vol. 91, 2009, pages 650 - 653, XP026040354, DOI: 10.1016/j.biochi.2009.01.014
OH H-JKIM H-JOH D-K: "Increase in d-tagatose production rate by site-directed mutagenesis of 1-arabinose isomerase from Geobacillus thermodenitrificans", BIOTECHNOL. LETT., vol. 28, 2006, pages 145 - 149, XP019231191, DOI: 10.1007/s10529-005-5327-4
BOSSHART AHEE CSBECHTOLD MSCHIRMER TPANKE S: "Directed divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substrates", CHEMBIOCHEM, vol. 16, 2015, pages 592 - 601, XP002789444
"Molecular Biology", JOHN WILEY AND SONS, INC., article "Modern Methods"
SAMBROOK ET AL.: "Metabolic Engineering of Microorganisms: Methods and Protocols", 1989, COLD SPRING HARBOR LABORATORY PRESS
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL., vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
ZHANG, X. Z.ZHANG, Y. H. P.: "Simple, fast and high-efficiency transformation system for directed evolution of cellulase", BACILLUS SUBTILIS. MICROB. BIOTECHNOL., vol. 4, no. 1, 2011, pages 98 - 105, XP055701512, DOI: 10.1111/j.1751-7915.2010.00230.x
RHIMI MAGHAJARI NJUY MCHOUAYEKH HMAGUIN EHASER RBEJAR S: "Rational design of Bacillus stearothermophilus US 1001-arabinose isomerase: Potential applications for d-tagatose production", BIOCHIM., vol. 91, 2009, pages 650 - 653, XP026040354, DOI: 10.1016/j.biochi.2009.01.014
OH H-JKIM H-JOH D-K: "Increase in d-tagatose production rate by site-directed mutagenesis of 1-arabinose isomerase from Geobacillus thermodenitrificans", BIOTECHNOL. LETT., vol. 28, 2006, pages 145 - 149, XP019231191, DOI: 10.1007/s10529-005-5327-4
BOSSHART AHEE CSBECHTOLD MSCHIRMER TPANKE S: "Directed divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substrates", CHEMBIOCHEM, vol. 16, 2015, pages 592 - 601, XP002789444
"Molecular Biology", JOHN WILEY AND SONS, INC., article "Modern Methods"
SAMBROOK ET AL.: "Metabolic Engineering of Microorganisms: Methods and Protocols", 1989, COLD SPRING HARBOR LABORATORY PRESS
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL., vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
ZHANG, X. Z.ZHANG, Y. H. P.: "Simple, fast and high-efficiency transformation system for directed evolution of cellulase", BACILLUS SUBTILIS. MICROB. BIOTECHNOL., vol. 4, no. 1, 2011, pages 98 - 105, XP055701512, DOI: 10.1111/j.1751-7915.2010.00230.x
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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