Title:
RECOMBINANT MICROORGANISM FOR PRODUCING 2,3-BUTANEDIOL WITH REDUCED BY-PRODUCT PRODUCTION AND METHOD FOR PRODUCING 2,3-BUTANEDIOL BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/211212
Kind Code:
A1
Abstract:
The present invention relates to a recombinant microorganism for producing 2,3-butanediol, in which, in a microorganism having the 2,3-butanediol biosynthetic pathway, at least one selected from the group consisting of a pathway for converting glutamic acid into polyglutamic acid, a pathway for converting sucrose into levan, a pathway for converting pyruvate into lactate, and a pathway for converting glycerophosphate into glycerol is inhibited. The present invention also relates to a method for producing 2,3-butanediol by using the recombinant microorganism for producing 2,3-butanediol.
Inventors:
SONG CHAN WOO (KR)
SONG HYO HAK (KR)
RATHNASINGH CHELLADURAI (KR)
PARK JONG MYOUNG (KR)
SONG HYO HAK (KR)
RATHNASINGH CHELLADURAI (KR)
PARK JONG MYOUNG (KR)
Application Number:
PCT/KR2021/017564
Publication Date:
October 06, 2022
Filing Date:
November 25, 2021
Export Citation:
Assignee:
GS CALTEX CORP (KR)
International Classes:
C12N15/75; C12N9/04; C12N9/06; C12N9/10; C12N9/16; C12P7/18
Foreign References:
| KR20140114726A | 2014-09-29 | |||
| KR20160098561A | 2016-08-19 |
Other References:
QINGZHAO WANG, TAO CHEN, XUEMING ZHAO, JAUHLEENE CHAMU: "Metabolic engineering of thermophilic Bacillus licheniformis for chiral pure D-2,3-butanediol production", BIOTECHNOLOGY AND BIOENGINEERING, JOHN WILEY, HOBOKEN, USA, vol. 109, no. 7, 1 July 2012 (2012-07-01), Hoboken, USA, pages 1610 - 1621, XP055355342, ISSN: 0006-3592, DOI: 10.1002/bit.24427
SCHILLING CHRISTOPH, CICCONE ROSARIO, SIEBER VOLKER, SCHMID JOCHEN: "Engineering of the 2,3-butanediol pathway of Paenibacillus polymyxa DSM 365", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 61, 1 September 2020 (2020-09-01), AMSTERDAM, NL, pages 381 - 388, XP055959946, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2020.07.009
KIM JIN-WOO; LEE YE-GI; KIM SOO-JUNG; JIN YONG-SU; SEO JIN-HO: "Deletion of glycerol-3-phosphate dehydrogenase genes improved 2,3-butanediol production by reducing glycerol production in pyruvate decarboxylase-deficient Saccharomyces cerevisiae", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 304, 14 August 2019 (2019-08-14), Amsterdam NL , pages 31 - 37, XP085788188, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2019.08.009
SONG CHAN WOO; RATHNASINGH CHELLADURAI; SONG HYOHAK: "CRISPR-Cas9 mediated metabolic engineering of a mucoid Bacillus licheniformis isolate for mass production of 2,3-butanediol", BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 175, 18 July 2021 (2021-07-18), NL , XP086720269, ISSN: 1369-703X, DOI: 10.1016/j.bej.2021.108141
JI ET AL., BIOTECHNOL. LETT., vol. 30, 2008, pages 731
FIG. MA ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 82, 2009, pages 49
SCHILLING CHRISTOPH, CICCONE ROSARIO, SIEBER VOLKER, SCHMID JOCHEN: "Engineering of the 2,3-butanediol pathway of Paenibacillus polymyxa DSM 365", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 61, 1 September 2020 (2020-09-01), AMSTERDAM, NL, pages 381 - 388, XP055959946, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2020.07.009
KIM JIN-WOO; LEE YE-GI; KIM SOO-JUNG; JIN YONG-SU; SEO JIN-HO: "Deletion of glycerol-3-phosphate dehydrogenase genes improved 2,3-butanediol production by reducing glycerol production in pyruvate decarboxylase-deficient Saccharomyces cerevisiae", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 304, 14 August 2019 (2019-08-14), Amsterdam NL , pages 31 - 37, XP085788188, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2019.08.009
SONG CHAN WOO; RATHNASINGH CHELLADURAI; SONG HYOHAK: "CRISPR-Cas9 mediated metabolic engineering of a mucoid Bacillus licheniformis isolate for mass production of 2,3-butanediol", BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 175, 18 July 2021 (2021-07-18), NL , XP086720269, ISSN: 1369-703X, DOI: 10.1016/j.bej.2021.108141
JI ET AL., BIOTECHNOL. LETT., vol. 30, 2008, pages 731
FIG. MA ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 82, 2009, pages 49
Attorney, Agent or Firm:
DAE-A INTELLECTUAL PROPERTY CONSULTING (KR)
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