Title:
RECOMBINANT PLASMID COMBINATION, GENETICALLY MODIFIED YEAST STRAIN, AND METHOD FOR PRODUCING AN ODD-CHAIN FATTY ACID.
Document Type and Number:
WIPO Patent Application WO/2021/114913
Kind Code:
A1
Abstract:
Disclosed are a recombinant plasmid combination, a genetically modified yeast strain, and a method for producing an odd chain fatty acid. The recombinant plasmid combination comprises at least one expression plasmid, the expression plasmid comprising at least one polynucleotide encoding a polypeptide having a threonine deaminase activity, a fatty acid synthase activity, a propionyl-CoA synthase activity, an aldehyde dehydrogenase activity, or a keto acid decarboxylase activity. The recombinant plasmid combination can be used to genetically modify a microorganism, such that the expression of key enzymes can be regulated so as to establish a synthesis route for synthesizing an odd-chain fatty acid in the modified microorganism. Propionic acid, or L-threonine and/or glucose can be used to directly synthesize the odd-chain fatty acid, thus keeping fermentation costs low. The yield and proportion of the odd chain fatty acid can be further increased by continual application of the genetic modification process.
Inventors:
SHI SHUOBO (CN)
MENG QIONGYU (CN)
DING WENTAO (CN)
MENG QIONGYU (CN)
DING WENTAO (CN)
Application Number:
PCT/CN2020/124018
Publication Date:
June 17, 2021
Filing Date:
October 27, 2020
Export Citation:
Assignee:
UNIV BEIJING CHEM TECH (CN)
International Classes:
C12N15/81; C12N1/16; C12P7/64
Domestic Patent References:
| WO2012096686A1 | 2012-07-19 |
Foreign References:
| CN103906845A | 2014-07-02 | |||
| CN103906845A | 2014-07-02 |
Other References:
VLAEMINCK, B. ET AL.: "Factors affecting odd- and branched-chain fatty acids in milk: A review.", ANIMAL FEED SCIENCE AND TECHNOLOGY., vol. 131, 31 December 2006 (2006-12-31), XP024934083, DOI: 10.1016/j.anifeedsci.2006.06.017
SHIMIZU, S. ET AL.: "Production of Odd Chain Polyunsaturated Fatty Acids by Mortierella fungi.", JAOCS., vol. 68, no. 4, 30 April 1991 (1991-04-30), XP055029734, DOI: 10.1007/BF02657620
KANEDA, T. ET AL.: "Iso- and Anteiso-Fatty Acids in Bacteria: Biosynthesis, Function, and Taxonomic Significance.", MICROBIOLOGICAL REVIEWS., vol. 55, no. 2, 30 June 1991 (1991-06-30), XP002627085
BENJAMIN JENKINS, JAMES WEST, ALBERT KOULMAN: "A Review of Odd-Chain Fatty Acid Metabolism and the Role of Pentadecanoic Acid (C15:0) and Heptadecanoic Acid (C17:0) in Health and Disease", MOLECULES, vol. 20, no. 2, pages 2425 - 2444, XP055258173, DOI: 10.3390/molecules20022425
王蕾 等 (WANG, LEI ET AL.): "乳脂中奇数和支链脂肪酸的研究进展 (Non-official translation: Research Progress on Odd and Branched-Chain Fatty Acids (OBCFA) in Milk Fat)", 饲料工业 (FEED INDUSTRY), vol. 29, no. 6, 31 December 2008 (2008-12-31)
SHIMIZU, S. ET AL.: "Production of Odd Chain Polyunsaturated Fatty Acids by Mortierella fungi.", JAOCS., vol. 68, no. 4, 30 April 1991 (1991-04-30), XP055029734, DOI: 10.1007/BF02657620
KANEDA, T. ET AL.: "Iso- and Anteiso-Fatty Acids in Bacteria: Biosynthesis, Function, and Taxonomic Significance.", MICROBIOLOGICAL REVIEWS., vol. 55, no. 2, 30 June 1991 (1991-06-30), XP002627085
BENJAMIN JENKINS, JAMES WEST, ALBERT KOULMAN: "A Review of Odd-Chain Fatty Acid Metabolism and the Role of Pentadecanoic Acid (C15:0) and Heptadecanoic Acid (C17:0) in Health and Disease", MOLECULES, vol. 20, no. 2, pages 2425 - 2444, XP055258173, DOI: 10.3390/molecules20022425
王蕾 等 (WANG, LEI ET AL.): "乳脂中奇数和支链脂肪酸的研究进展 (Non-official translation: Research Progress on Odd and Branched-Chain Fatty Acids (OBCFA) in Milk Fat)", 饲料工业 (FEED INDUSTRY), vol. 29, no. 6, 31 December 2008 (2008-12-31)
Attorney, Agent or Firm:
SUNSHINE INTELLECTUAL PROPERTY INTERNATIONAL CO., LTD. (CN)
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