Title:
RECOMBINANT POLYPEPTIDE HAVING ACYL-COA COMPOUND REDUCING ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2022/209994
Kind Code:
A1
Abstract:
[Problem] To provide a recombinant polypeptide having excellent acyl-CoA compound reducing activity and a method for producing an aliphatic compound using the recombinant polypeptide. [Solution] A recombinant polypeptide characterized by: (a) comprising amino acid sequence A that has a sequence identity of 60% or higher, 65% or higher, 70% or higher, 75% or higher, 80% or higher, 85% or higher, 88% or higher, 90% or higher, 93% or higher, 95% or higher, 97% or higher, 98% or higher or 99% or higher with the amino acid sequence shown in SEQ ID NO: 1; (b) said amino acid sequence A having a substitution at at least one amino acid at the position corresponding to the substrate-binding site of a polypeptide that comprises the amino acid sequence shown in SEQ ID NO: 1; and (c) having reducing activity R of converting CoA thioester of an acyl-CoA compound into an aldehyde group, said reducing activity R including (c-1) reducing activity R1 of converting adipyl-CoA into 5-formylpentanoic acid in one step, and (c-2) reducing activity R2 of converting succinyl-CoA into succinic acid semialdehyde.
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Inventors:
ISAKA KOJI (JP)
MIYATAKE RYO (JP)
KATAYAMA SAYAKA (JP)
MIYATAKE RYO (JP)
KATAYAMA SAYAKA (JP)
Application Number:
PCT/JP2022/012463
Publication Date:
October 06, 2022
Filing Date:
March 17, 2022
Export Citation:
Assignee:
ASAHI CHEMICAL IND (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12N9/10; C12N15/63; C12P7/42
Domestic Patent References:
| WO2019159831A1 | 2019-08-22 |
Foreign References:
| JP2012522010A | 2012-09-20 | |||
| JP2018500911A | 2018-01-18 | |||
| JP2016533162A | 2016-10-27 | |||
| JP2017533734A | 2017-11-16 | |||
| JP2012511910A | 2012-05-31 | |||
| JP2018046843A | 2018-03-29 | |||
| JP2012188407A | 2012-10-04 | |||
| JP2012525856A | 2012-10-25 | |||
| JP2016538870A | 2016-12-15 | |||
| JP2016501031A | 2016-01-18 | |||
| JP2017533734A | 2017-11-16 | |||
| JP2016533162A | 2016-10-27 | |||
| JP2011512868A | 2011-04-28 |
Other References:
DATABASE UNIPROTKB 25 April 2018 (2018-04-25), ANONYMOUS : "A0A2N2DQ45 · A0A2N2DQ45_9FIRM", XP055973495, retrieved from UNIPROT Database accession no. A0A2N2DQ45
TURK STEFAN C. H. J., KLOOSTERMAN WIGARD P., NINABER DENNIS K., KOLEN KARIN P. A. M., KNUTOVA JULIA, SUIR ERWIN, SCHÜRMANN MARTIN,: "Metabolic Engineering toward Sustainable Production of Nylon-6", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 5, no. 1, 15 January 2016 (2016-01-15), Washington DC ,USA , pages 65 - 73, XP055973496, ISSN: 2161-5063, DOI: 10.1021/acssynbio.5b00129
ANONYMOUS: "UPLOAD TOYOBO BIOCHEMICALS ", TOYOBO, 31 August 2007 (2007-08-31), XP055973497, Retrieved from the Internet [retrieved on 20221021]
YU: "Biotechnology and Bioengineering", vol. 111, 2014, WILEY PERIODICALS, INC., article "Direct biosynthesis of adipic acid from a synthetic pathway in recombinant Escherichia coli", pages: 2580
YU ZHOU: "Journal of Biotechnology", vol. 8, 2020, ELSEVIER, article "Biosynthesis of adipic acid by a highly efficient induction-free system in Escherichia Coli", pages: 314 - 315
CHEONG: "Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions", NATURE BIOTECHNOLOGY, 2016
TURK: "Metabolic Engineering toward Sustainable Production of Nylon-6", ACS SYNTH. BIOL., vol. 5, 2016, pages 65 - 73, XP055973496, DOI: 10.1021/acssynbio.5b00129
"Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
Greene Publishing Associates and Wiley-Interscience; "Genebank", Database accession no. NZ CP019870.1
"Gene Cloning and DNA analysis 6th edition", 2016, WILEY- BLACKWELL
"Gene Expression Technology: Methods in Enzymology", 1990, ACADEMIC PRESS, pages: 185
"NCBI", Database accession no. AP009048
MANNING ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018
TURK STEFAN C. H. J., KLOOSTERMAN WIGARD P., NINABER DENNIS K., KOLEN KARIN P. A. M., KNUTOVA JULIA, SUIR ERWIN, SCHÜRMANN MARTIN,: "Metabolic Engineering toward Sustainable Production of Nylon-6", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 5, no. 1, 15 January 2016 (2016-01-15), Washington DC ,USA , pages 65 - 73, XP055973496, ISSN: 2161-5063, DOI: 10.1021/acssynbio.5b00129
ANONYMOUS: "UPLOAD TOYOBO BIOCHEMICALS ", TOYOBO, 31 August 2007 (2007-08-31), XP055973497, Retrieved from the Internet
YU: "Biotechnology and Bioengineering", vol. 111, 2014, WILEY PERIODICALS, INC., article "Direct biosynthesis of adipic acid from a synthetic pathway in recombinant Escherichia coli", pages: 2580
YU ZHOU: "Journal of Biotechnology", vol. 8, 2020, ELSEVIER, article "Biosynthesis of adipic acid by a highly efficient induction-free system in Escherichia Coli", pages: 314 - 315
CHEONG: "Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions", NATURE BIOTECHNOLOGY, 2016
TURK: "Metabolic Engineering toward Sustainable Production of Nylon-6", ACS SYNTH. BIOL., vol. 5, 2016, pages 65 - 73, XP055973496, DOI: 10.1021/acssynbio.5b00129
"Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
Greene Publishing Associates and Wiley-Interscience; "Genebank", Database accession no. NZ CP019870.1
"Gene Cloning and DNA analysis 6th edition", 2016, WILEY- BLACKWELL
"Gene Expression Technology: Methods in Enzymology", 1990, ACADEMIC PRESS, pages: 185
"NCBI", Database accession no. AP009048
MANNING ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
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