RECOMBINANT POLYPEPTIDE HAVING CARBOXYLIC ACID REDUCING ACTIVITY

WIPO Patent Application WO/2022/209763

A1

[Problem] To provide a recombinant polypeptide having excellent carboxylic acid reducing activity and a method for producing an aliphatic compound using the recombinant polypeptide. [Solution] A recombinant polypeptide characterized by: (a) comprising amino acid sequence A that has a sequence identity of 60% or higher, 65% or higher, 70% or higher, 75% or higher, 80% or higher, 85% or higher, 88% or higher, 90% or higher, 95% or higher, 97% or higher, 98% or higher or 99% or higher with the amino acid sequence shown in SEQ ID NO: 1; (b) said amino acid sequence A having a substitution at at least one amino acid at the position corresponding to the substrate-binding site of a polypeptide that comprises the amino acid sequence shown in SEQ ID NO: 1; and (c) having carboxylic acid reducing activity.

ISAKA KOJI (JP)
MIYATAKE RYO (JP)
KATAYAMA SAYAKA (JP)

PCT/JP2022/010912

October 06, 2022

March 11, 2022

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ASAHI CHEMICAL IND (JP)

C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N15/31; C12N15/63; C12P7/02; C12P7/24; C12P13/00; C12N15/10

WO2012177721A1	2012-12-27
WO2014095986A1	2014-06-26

JP2015512645A	2015-04-30
US5573931A	1996-11-12
JP2012525856A	2012-10-25
JP2016538870A	2016-12-15
JP2016501031A	2016-01-18
JP2017533734A	2017-11-16
JP2016533162A	2016-10-27
JP2011254747A	2011-12-22
JP2015512645A	2015-04-30
US20200080064A1	2020-03-12

TATIANA P. FEDORCHUK, ANNA N. KHUSNUTDINOVA, ELENA EVDOKIMOVA, ROBERT FLICK, ROSA DI LEO, PETER STOGIOS, ALEXEI SAVCHENKO, ALEXAND: "One-Pot Biocatalytic Transformation of Adipic Acid to 6-Aminocaproic Acid and 1,6-Hexamethylenediamine Using Carboxylic Acid Reductases and Transaminases", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 142, no. 2, 15 January 2020 (2020-01-15), pages 1038 - 1048, XP055711997, ISSN: 0002-7863, DOI: 10.1021/jacs.9b11761
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ONO Makoto et al. (JP)

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