Title:
RECOMBINANT PRODUCTION OF IMMUNOGLOBULIN-LIKE DOMAINS IN PROKARYOTIC CELLS
Document Type and Number:
WIPO Patent Application WO1993022332
Kind Code:
A3
Abstract:
Disclosed are recombinant vectors encoding immunoglobulin-like domains and portions thereof, such as T-cell variable domains, antibody Fc-hinge fragments, subfragments and mutant domains with reduced biological half lives. Methods of producing large quantities of such domains, heterodimers, and fusion proteins following expression and secretion by prokaryotic host cells are also reported. Described are single chain T-cell receptors, which are folded into beta -pleated sheet structures similar to those of immunoglobulin variable domains; antibody Fc and Fc-hinge domains, which have the same in vivo stability as intact antibodies; and domains engineered to have reduced half lives.
Inventors:
WARD ELIZABETH SALLY (US)
KIM JIN-KYOO (US)
KIM JIN-KYOO (US)
Application Number:
PCT/US1993/003895
Publication Date:
February 17, 1994
Filing Date:
April 26, 1993
Export Citation:
Assignee:
UNIV TEXAS (US)
International Classes:
A61K47/48; C07K14/705; C07K14/725; C07K14/73; C07K14/735; C07K14/74; C07K16/00; C12N1/21; C12P21/04; (IPC1-7): C07H23/00; C12Q1/68; C12P19/34; C12P21/04; C12N15/00; C12N1/20; C12N1/08; C12N1/00
Domestic Patent References:
| WO1991008298A2 | 1991-06-13 | |||
| WO1992001787A1 | 1992-02-06 |
Foreign References:
| EP0368684A1 | 1990-05-16 |
Other References:
E.S. WARD: "Expression and secretion of T-cell receptor V-alpha and V-beta domains using Escherichia coli as a host", SCANDINAVIAN JOURNAL OF IMMUNOLOGY, vol. 34, no. 2, August 1991 (1991-08-01), pages 215 - 220
E. SALLY WARD ET AL.: "Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli", NATURE, vol. 341, no. 6242, 12 October 1989 (1989-10-12), LONDON GB, pages 544 - 546
PATENT ABSTRACTS OF JAPAN vol. 13, no. 101 (C - 574)<3449> 9 March 1989 (1989-03-09)
MARK SUTER ET AL.: "Rabbit single domain antibodies specific to protein C expressed in prokaryotes", IMMUNOLOGY LETTERS, vol. 33, no. 1, June 1992 (1992-06-01), pages 53 - 60
E. SALLY WARD ET AL.: "Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli", NATURE, vol. 341, no. 6242, 12 October 1989 (1989-10-12), LONDON GB, pages 544 - 546
PATENT ABSTRACTS OF JAPAN vol. 13, no. 101 (C - 574)<3449> 9 March 1989 (1989-03-09)
MARK SUTER ET AL.: "Rabbit single domain antibodies specific to protein C expressed in prokaryotes", IMMUNOLOGY LETTERS, vol. 33, no. 1, June 1992 (1992-06-01), pages 53 - 60
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