Title:
RECOMBINANT SARS-COV-2 SPIKE PROTEIN SUBUNITS, EXPRESSION AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/051425
Kind Code:
A3
Abstract:
The present invention is directed to the expression and secretion recombinant SARS‑CoV‑2 spike protein subunits. Various subunits have been designed and expressed as secreted products into the culture medium of transformed insect cell lines. The design of subunits is focused on the production of products that provide the ability to induce focused immune responses without inducing immune enhancing responses. The expressed and purified products are suitable as vaccine candidates to protect against disease caused by SARS‑CoV‑2.
Inventors:
CLEMENTS DAVID E (US)
SENDA JAMES T (US)
HORTON JAIME S (US)
SENDA JAMES T (US)
HORTON JAIME S (US)
Application Number:
PCT/US2021/048750
Publication Date:
April 21, 2022
Filing Date:
September 01, 2021
Export Citation:
Assignee:
HAWAII BIOTECH INC (US)
International Classes:
C07K14/165; A61K39/215; C12N7/04; C12N15/85
Domestic Patent References:
| WO2018022790A1 | 2018-02-01 |
Other References:
SAMRAT SUBODH KUMAR; THARAPPEL ANIL M.; LI ZHONG; LI HONGMIN: "Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development", VIRUS RESEARCH, AMSTERDAM, NL, vol. 288, 23 August 2020 (2020-08-23), NL , XP086262756, ISSN: 0168-1702, DOI: 10.1016/j.virusres.2020.198141
WU FAN; ZHAO SU; YU BIN; CHEN YAN-MEI; WANG WEN; SONG ZHI-GANG; HU YI; TAO ZHAO-WU; TIAN JUN-HUA; PEI YUAN-YUAN; YUAN MING-LI; ZHA: "A new coronavirus associated with human respiratory disease in China", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 579, no. 7798, 3 February 2020 (2020-02-03), London, pages 265 - 269, XP037060203, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2008-3
SIT THOMAS H. C.; BRACKMAN CHRISTOPHER J.; IP SIN MING; TAM KARINA W. S.; LAW PIERRA Y. T.; TO ESTHER M. W.; YU VERONICA Y. T.; SI: "Infection of dogs with SARS-CoV-2", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 586, no. 7831, 14 May 2020 (2020-05-14), London, pages 776 - 778, XP037282852, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2334-5
HOLLAND LARINDA A., KAELIN EMILY A., MAQSOOD RABIA, ESTIFANOS BEREKET, WU LILY I., VARSANI ARVIND, HALDEN ROLF U., HOGUE BRENDA G.: "An 81-Nucleotide Deletion in SARS-CoV-2 ORF7a Identified from Sentinel Surveillance in Arizona (January to March 2020)", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 1, no. 3, 1 July 2020 (2020-07-01), US , pages 1 - 3, XP055934632, ISSN: 0022-538X, DOI: 10.1128/JVI.00711-20
FUJITA RYOSUKE; HINO MASATO; EBIHARA TAKERU; NAGASATO TAKUMI; MASUDA AKITSU; LEE JAE MAN; FUJII TSUGURU; MON HIROAKI; KAKINO KOHEI: "Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 529, no. 2, 9 June 2020 (2020-06-09), Amsterdam NL , pages 257 - 262, XP086221467, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2020.06.020
WU FAN; ZHAO SU; YU BIN; CHEN YAN-MEI; WANG WEN; SONG ZHI-GANG; HU YI; TAO ZHAO-WU; TIAN JUN-HUA; PEI YUAN-YUAN; YUAN MING-LI; ZHA: "A new coronavirus associated with human respiratory disease in China", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 579, no. 7798, 3 February 2020 (2020-02-03), London, pages 265 - 269, XP037060203, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2008-3
SIT THOMAS H. C.; BRACKMAN CHRISTOPHER J.; IP SIN MING; TAM KARINA W. S.; LAW PIERRA Y. T.; TO ESTHER M. W.; YU VERONICA Y. T.; SI: "Infection of dogs with SARS-CoV-2", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 586, no. 7831, 14 May 2020 (2020-05-14), London, pages 776 - 778, XP037282852, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2334-5
HOLLAND LARINDA A., KAELIN EMILY A., MAQSOOD RABIA, ESTIFANOS BEREKET, WU LILY I., VARSANI ARVIND, HALDEN ROLF U., HOGUE BRENDA G.: "An 81-Nucleotide Deletion in SARS-CoV-2 ORF7a Identified from Sentinel Surveillance in Arizona (January to March 2020)", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 1, no. 3, 1 July 2020 (2020-07-01), US , pages 1 - 3, XP055934632, ISSN: 0022-538X, DOI: 10.1128/JVI.00711-20
FUJITA RYOSUKE; HINO MASATO; EBIHARA TAKERU; NAGASATO TAKUMI; MASUDA AKITSU; LEE JAE MAN; FUJII TSUGURU; MON HIROAKI; KAKINO KOHEI: "Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 529, no. 2, 9 June 2020 (2020-06-09), Amsterdam NL , pages 257 - 262, XP086221467, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2020.06.020
Attorney, Agent or Firm:
HAILE, Lisa A. et al. (US)
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